MABE1885-25UG Anti-Histone H1 Antibody, clone 1G6

MABE1885-25UG
25 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Dr ChIP, ChIP-seq, IP, WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABE1885-25UG
      DescriptionAnti-Histone H1 Antibody, clone 1G6
      Alternate Names
      • H1
      Background InformationHistone H1 (UniProt: P02255; also known as H1) is encoded by the His 1 gene (Gene ID: 318854) in Drosophila species. Histone H1 is a linker histone that is involved in the organization and maintenance of chromatin higher order structure and plays an active role in the control of DNA replication and cell proliferation. The linker histone H1 family members are a key component of chromatin and bind to the nucleosomal core particle around the DNA entry and exit sites. Histone H1 is essential for the condensation of nucleosome chains into higher-order structures. In metazoans, H1 histones are smaller in size with about 200 amino acids. They have a short N-terminal tail, a central globular domain, and a long basic C-terminal tail. The central globular domain is shown to be highly conserved among all H1 subtypes. Manipulating levels of H1 histone can lead to both up- and down-regulation of specific genes. Depletion of H1 results in a massively altered chromosome structure with loss of chromosome banding. In Drosophila, a 20% reduction in H1 content leads to cessation of growth beyond the larval stage. Histone H1 undergoes phosphorylation mainly in the tail region, especially the C-terminal tail, where several {(S/T)-P-X-(K/R)} motifs are located that are recognized by cyclin-dependent kinases (CDKs). Phosphorylation levels are reported to be lowest during the G1 phase of the cell cycle and increase during S phase and reach maximum levels in mitosis. (Ref.: Hergeth, SP., and Schneider, R (2015). EMBO Rep. 16(11); 1439-1453).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Histone H1, clone 1G6 Antibody, Cat. No. MABE1885, is a highly specific mouse monoclonal antibody that targets Drosophila Histone H1 and has been tested for use in Chromatin Immunoprecipitation (ChIP), ChIP-seq, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Chromatin Immunoprecipitation (ChIP)
      • ChIP-seq
      • Immunoprecipitation
      • Western Blotting
      Application NotesChIP-seq Analysis: A representative lot detected Histone H1 in ChIP-seq applications (Iwasaki, Y.W., et. al. (2016). Mol Cell. 63(3):408-19).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated Histone H1 in Immunoprecipitation applications (Iwasaki, Y.W., et. al. (2016). Mol Cell. 63(3):408-19).

      Chromatin Immunoprecipitation Analysis (ChIP): A representative lot immunoprecipitated Histone H1 in Chromatin Immunoprecipitation applications (Iwasaki, Y.W., et. al. (2016). Mol Cell. 63(3):408-19).
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 148 amino acids from the C-terminal half of Drosophila Histone H1.
      EpitopeC-terminus
      Clone1G6
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 1G6 is a mouse monoclonal antibody that detects Histone H1 in Drosophila species.
      IsotypeIgG1κ
      Species Reactivity
      • Drosophila
      Species Reactivity NoteDrosophila.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • His 1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~35 kDa observed; 26.36 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Drosophila S2 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Histone H1 in Drosophila S2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications