PC319L Anti-Heparin Binding Epidermal Growth Factor (Ab-1) Goat pAb

PC319L
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H Gt Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      PC319L-100UG
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      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
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      Available
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          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes the ~19-23 kDa HB-EGF protein in vascular smooth muscle cells.
          Catalogue NumberPC319L
          Brand Family Calbiochem®
          SynonymsAnti-HB-EGF
          References
          ReferencesDavis-Fleischer, K.M. et al. 1998. Front. Biosci. 3, D288.
          Ouchi, N., et al. 1997. Biochem. J. 328, 923.
          Raab, G. and Klagsbrun M. 1997. Biochim. Biophys. Acta 1333, F179.
          Suzuki, M., et al. 1997. J. Biol. Chem. 272, 31730.
          Nakata, A., et al. 1996. Circulation 94, 2778.
          Miyagawa, J., et al. 1995. J. Clin. Invest. 95, 404.
          Product Information
          FormLyophilized
          FormulationLyophilized from sterile filtered solution in PBS, 5% trehalose, 100 µg BSA.
          Positive controlVascular smooth muscle cells
          PreservativeNone
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Neutralization Studies
          Application NotesELISA (0.5-1 µg/ml, see comments)
          Immunoblotting (1-2 µg/ml)
          Neutralization Studies (see comments)
          Application CommentsThis antibody has been selected for its ability to neutralize the biological activity of human recombinant HB-EGF. It will not neutralize the biological activity of EGF, TGF-α or amphiregulin. The exact concentration of antibody required to neutralize human recombinant HB-EGF activity is dependent on the cytokine concentration, cell type, growth conditions, and the type of activity studied. Suggested neutralization concentration required to yield one-half maximal inhibition of HB-EGF activity is ~3-6 µg/ml in the presence of 10 ng/ml of human recombinant HB-EGF using a Balb/3T3 cell line. For immunoblotting, the detection limit for human recombinant HB-EGF is ~0.5 ng/lane under non-reducing and reducing conditions. For ELISA, the detection limit for human recombinant HB-EGF is ~0.03 ng/well. This antibody exhibits no cross-reactivity with other cytokines when tested in ELISA. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenpurified, recombinant, human HB-EGF
          ImmunogenHuman
          HostGoat
          IsotypeIgG
          Species Reactivity
          • Human
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Harmful
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 0.25 mg/ml. Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Avoid freeze/thaw cycles of solutions.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-Heparin Binding Epidermal Growth Factor (Ab-1) Goat pAb SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-Heparin Binding Epidermal Growth Factor (Ab-1) Goat pAb Certificates of Analysis

          TitleLot Number
          PC319L

          References

          Reference overview
          Davis-Fleischer, K.M. et al. 1998. Front. Biosci. 3, D288.
          Ouchi, N., et al. 1997. Biochem. J. 328, 923.
          Raab, G. and Klagsbrun M. 1997. Biochim. Biophys. Acta 1333, F179.
          Suzuki, M., et al. 1997. J. Biol. Chem. 272, 31730.
          Nakata, A., et al. 1996. Circulation 94, 2778.
          Miyagawa, J., et al. 1995. J. Clin. Invest. 95, 404.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision09-October-2007 RFH
          SynonymsAnti-HB-EGF
          ApplicationELISA (0.5-1 µg/ml, see comments)
          Immunoblotting (1-2 µg/ml)
          Neutralization Studies (see comments)
          DescriptionPurified goat polyclonal antibody. Recognizes the ~19-23 kDa (apparent MW) heparin binding epidermal growth factor protein.
          BackgroundHeparin-binding epidermal growth factor-like growth factor (HB-EGF) is a ~22 kDa O-glycosylated protein that is a potent mitogen and chemoattractant for vascular smooth muscle cells, fibroblasts and epithelial cells but not endothelial cells. The natural protein has an apparent molecular mass of ~19-23 kDa and exists in multiple forms as a result of heterogeneous O-glycosylation and/or N-terminal truncation. HB-EGF is synthesized as a membrane-anchored precursor (proHB-EGF) that is proteolytically cleaved to release the soluble mature growth factor. The two forms are active as juxtacrine and paracrine/autocrine growth factors respectively. Recent studies show MMP-3 as one of the enzymes that cleaves HB-EGF implicating a role for MMP-3 in the regulation of HB-EGF from being a juxtacrine to a paracrine/autocrine factor. HB-EGF activates two EGF receptor subtypes, HER1/ErbB1 and HER4 and binds to heparan sulfate proteoglycan. The proHB-EGF is postulated as the high affinity receptor for diphtheria toxin (DT). Recent evidence suggests that the coexpression of proHB-EGF and CD9 on macrophages may strongly promote the development of atherosclerosis by a juxtacrine mechanism. Transcription of HB-EGF can be induced in vascular endothelial cells as well as aortic smooth muscle cells which may also play an important role in the pathogenesis of atherosclerosis.
          HostGoat
          Immunogen speciesHuman
          Immunogenpurified, recombinant, human HB-EGF
          IsotypeIgG
          Specieshuman
          Positive controlVascular smooth muscle cells
          FormLyophilized
          FormulationLyophilized from sterile filtered solution in PBS, 5% trehalose, 100 µg BSA.
          PreservativeNone
          CommentsThis antibody has been selected for its ability to neutralize the biological activity of human recombinant HB-EGF. It will not neutralize the biological activity of EGF, TGF-α or amphiregulin. The exact concentration of antibody required to neutralize human recombinant HB-EGF activity is dependent on the cytokine concentration, cell type, growth conditions, and the type of activity studied. Suggested neutralization concentration required to yield one-half maximal inhibition of HB-EGF activity is ~3-6 µg/ml in the presence of 10 ng/ml of human recombinant HB-EGF using a Balb/3T3 cell line. For immunoblotting, the detection limit for human recombinant HB-EGF is ~0.5 ng/lane under non-reducing and reducing conditions. For ELISA, the detection limit for human recombinant HB-EGF is ~0.03 ng/well. This antibody exhibits no cross-reactivity with other cytokines when tested in ELISA. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 0.25 mg/ml. Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Avoid freeze/thaw cycles of solutions.
          Toxicity Harmful
          ReferencesDavis-Fleischer, K.M. et al. 1998. Front. Biosci. 3, D288.
          Ouchi, N., et al. 1997. Biochem. J. 328, 923.
          Raab, G. and Klagsbrun M. 1997. Biochim. Biophys. Acta 1333, F179.
          Suzuki, M., et al. 1997. J. Biol. Chem. 272, 31730.
          Nakata, A., et al. 1996. Circulation 94, 2778.
          Miyagawa, J., et al. 1995. J. Clin. Invest. 95, 404.