ST1529 Anti-HTT Mouse mAb (3F1)

ST1529
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service
      View Pricing & Availability

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      ST1529-100UG
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          100 μg
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          OverviewRecognizes the ~50 kDa HTT protein in U-2 OS and HeLa cells and human salivary gland tissue.
          Catalogue NumberST1529
          Brand Family Calbiochem®
          SynonymsAnti-HD, Anti-Huntingtin
          Application Data
          Detection of human HTT by immunoblotting. Sample: Whole cell lysate from U-2 OS cells. Primary antibody: Anti-HTT Mouse mAb (3F1) (Cat. No. ST1529) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human HTT by immunoblotting. Sample: Whole cell lysate from HeLe NE cells. Primary antibody: Anti-HTT Mouse mAb (3F1) (Cat. No. ST1529) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human HTT by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human salivary gland tissue. Primary antibody: Anti-HTT Mouse mAb (3F1) (Cat. No. ST1529) (3 µg/ml). Detection: colorimetric (DAB).
          References
          Product Information
          FormLiquid
          FormulationIn PBS, pH 7.2.
          Positive controlU-2 OS cells, HeLa NE cells, Human salivary gland tissue
          PreservativeNone
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Paraffin Sections
          Application NotesELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Paraffin Sections (3 µg/ml)
          Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          ImmunogenA recombinant polypeptide corresponding to amino acids 81-191 of human HTT, expressed as a GST fusion protein
          Clone3F1
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Regulatory Review
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-HTT Mouse mAb (3F1) SDS

          Title

          Safety Data Sheet (SDS) 

          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision08-November-2010 RFH
          SynonymsAnti-HD, Anti-Huntingtin
          ApplicationELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Paraffin Sections (3 µg/ml)
          Application Data
          Detection of human HTT by immunoblotting. Sample: Whole cell lysate from U-2 OS cells. Primary antibody: Anti-HTT Mouse mAb (3F1) (Cat. No. ST1529) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human HTT by immunoblotting. Sample: Whole cell lysate from HeLe NE cells. Primary antibody: Anti-HTT Mouse mAb (3F1) (Cat. No. ST1529) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human HTT by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human salivary gland tissue. Primary antibody: Anti-HTT Mouse mAb (3F1) (Cat. No. ST1529) (3 µg/ml). Detection: colorimetric (DAB).
          DescriptionPurified mouse monoclonal antibody. Recognizes the ~50 kDa HTT protein.
          BackgroundHuntington's disease is a gene-linked neurodegenerative disorder characterized by loss of striatal neurons. This disorder is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. A fairly broad range in the number of trinucleotide repeats has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological. The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is ~13.7 kb and is expressed predominantly in adult and fetal brain, whereas the smaller transcript is ~10.3 kb and is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats.
          HostMouse
          ImmunogenA recombinant polypeptide corresponding to amino acids 81-191 of human HTT, expressed as a GST fusion protein
          Clone3F1
          IsotypeIgG2a
          Specieshuman
          Positive controlU-2 OS cells, HeLa NE cells, Human salivary gland tissue
          FormLiquid
          FormulationIn PBS, pH 7.2.
          PreservativeNone
          CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Regulatory Review