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MABC576 | Anti-HOIL-1 Antibody, clone 2E2

100 μg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M WB, ICC M Purified Monoclonal Antibody
      Catalogue NumberMABC576
      DescriptionAnti-HOIL-1 Antibody, clone 2E2
      Alternate Names
      • RanBP-type and C3HC4-type zinc finger-containing protein 1
      • HBV-associated factor 4
      • Heme-oxidized IRP2 ubiquitin ligase 1
      • HOIL-1
      • Hepatitis B virus X-associated protein 4
      • RING finger protein 54
      • Ubiquitin-conjugating enzyme 7-interacting protein 3
      • HOIL-1
      Background InformationRanBP-type and C3HC4-type zinc finger-containing protein 1 (UniProt Q9BYM8; also known as HOIL-1, HBV-associated factor 4, Heme-oxidized IRP2 ubiquitin ligase 1, Hepatitis B virus X-associated protein 4, RBCC protein interacting with PKC1, RING finger protein 54, Ubiquitin-conjugating enzyme 7-interacting protein 3) is encoded by the RBCK1 gene (also known as C20orf18, HOIL1, PBMEI, RBCK2, RNF54, UBCE7IP3, XAP3, XAP4, ZRANB4) (Gene ID 10616) in human. The linear ubiquitin assembly complex (LUBAC) is comprised of two RING-IBR-RING (RBR)-containing E3 ligases, HOIL-1L and HOIP. LUBAC conjugates a head-to-tail-linked linear polyubiquitin chain onto lysine residues on substrate proteins. Ovarian tumor deubiquitinating enzyme (DUB) with linear linkage specificity (OTULIN/Gumby/FAM105B) and cylindromatosis (CYLD) are two DUBs that catalyze the cleavage linear polyubiquitin chains. OTULIN and CYLD interact with the N-terminal PNGase/UBA or UBX (PUB) domain of HOIP and negatively regulate LUBAC-mediated cellular processes. LUBAC is reported to suppress IFN-dependent antiviral activity by mediating TRIM25 degradation and competing against TRIM25 for RIG-I interaction.
      Product Information
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationThis Anti-HOIL-1 Antibody, clone 2E2 is validated for use in Western Blotting, Immunocytochemistry for the detection of HOIL-1 .
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: A representative lot detected HOIL-1 in HeLa, SH-SY5Y, and U20S cells (Kirisako, T., et al (2006). EMBO J. 25(20):48877-4887).
      Western Blotting Analysis: A representative lot detected HOIL-1 in mouse liver extracts (Tokunaga, F., et al (2009). Nat. Cell Biol. 11(2):123-132).
      Western Blotting Analysis: A representative lot detected HOIL-1 in MEFs (Takiuchi, T., et al (2014). Genes Cells. 19(3):254-272).
      Immunocytochemistry Analysis: A representative lot detected HOIL-1 in HeLa cells with or without Sendai virus (SeV) infection (Inn, K.S., et al. (2011). Mol Cell. 41(3):354-365).
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human HOIL-1 near the C-terminus.
      EpitopeNear C-terminus
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityClone 2E2 reacts with isoform 1 (HOIL-1L) and isoform 2, but not isoform 3.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RBCK1
      • C20orf18
      • RNF54
      • UBCE7IP3
      • XAP3
      • XAP4
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~57 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in THP-1 cell lysate.

      Western Blotting Analysis: 2.0 µg/mL of this antibody detected HOIL-1 in 10 µg of THP-1 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-HOIL-1, clone 2E2 -Q2574782 Q2574782


      Reference overviewPub Med ID
      Suppression of LUBAC-mediated linear ubiquitination by a specific interaction between LUBAC and the deubiquitinases CYLD and OTULIN.
      Takiuchi, T; Nakagawa, T; Tamiya, H; Fujita, H; Sasaki, Y; Saeki, Y; Takeda, H; Sawasaki, T; Buchberger, A; Kimura, T; Iwai, K
      Genes to cells : devoted to molecular & cellular mechanisms 19 254-72 2014

      Show Abstract
      24461064 24461064
      Pathogen blocks host death receptor signalling by arginine GlcNAcylation of death domains.
      Li, S; Zhang, L; Yao, Q; Li, L; Dong, N; Rong, J; Gao, W; Ding, X; Sun, L; Chen, X; Chen, S; Shao, F
      Nature 501 242-6 2013

      Show Abstract
      23955153 23955153
      Linear ubiquitin assembly complex negatively regulates RIG-I- and TRIM25-mediated type I interferon induction.
      Inn, KS; Gack, MU; Tokunaga, F; Shi, M; Wong, LY; Iwai, K; Jung, JU
      Molecular cell 41 354-65 2011

      Show Abstract
      21292167 21292167
      Involvement of linear polyubiquitylation of NEMO in NF-kappaB activation.
      Tokunaga, F; Sakata, S; Saeki, Y; Satomi, Y; Kirisako, T; Kamei, K; Nakagawa, T; Kato, M; Murata, S; Yamaoka, S; Yamamoto, M; Akira, S; Takao, T; Tanaka, K; Iwai, K
      Nature cell biology 11 123-32 2009

      Show Abstract
      19136968 19136968
      A ubiquitin ligase complex assembles linear polyubiquitin chains.
      Kirisako, T; Kamei, K; Murata, S; Kato, M; Fukumoto, H; Kanie, M; Sano, S; Tokunaga, F; Tanaka, K; Iwai, K
      The EMBO journal 25 4877-87 2006

      Show Abstract
      17006537 17006537

      Technical Info

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