393207 Anti-HNE-Michael Adducts, Reduced Rabbit pAb

393207
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      A Broad Range Of Species Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      393207-100UL
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          Plastic ampoule 100 ul
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          Description
          OverviewRecognizes chemically reduced amino acid-(4)-HNE adducts. Does not recognize non-reduced adducts or reduced modifications arising from malondialdehyde (MDA) and structurally-related alkenes such as trans-2-noneal.
          Catalogue Number393207
          Brand Family Calbiochem®
          SynonymsAnti-4-Hydroxy-2-noneal Michael Adducts, Reduced
          References
          ReferencesToyokuni, S., et al. 2000. Antioxid. Redox. Signal 2, 681.
          Neely, M.D., et al. 1999. J. Neurochem. 72, 2323.
          Uchida, K., et al. 1995. Biochem. Biophys. Res. Commun. 212, 1068.
          Uchida, K., et al. 1995. Arch. Biochem. Biophys. 324, 241.
          Uchida, K., et al. 1994. Biochemistry 33, 12487.
          Product Information
          FormLiquid
          FormulationSerum diluted in 50% glycerol, pH 7.5.
          PreservativeNone
          Applications
          Key Applications Paraffin Sections
          Enzyme-Linked Immunosorbent Assay
          Frozen Sections
          Immunoblotting (Western Blotting)
          Application NotesELISA (1:500-1:1000)
          Immunoblotting (1:1000-1:4000)
          Frozen Sections (1:50-1:500)
          Paraffin Sections (1:50-1:500)
          Application CommentsDoes not bind non-reduced adducts and reduced modifications arising from malondialdehyde (MDA) or structurally-related alkenals such as trans-2-noneal. Does not cross-react with non-reduced amino acid-HNE Michael adducts. Antibody binding requires the presence of the 4-hydroxyl group. Variables associated with assay conditions will dictate the optimal working dilution.
          Biological Information
          ImmunogenHNE-treated KLH in which 1:1 amino acid-HNE Michael adducts were chemically reduced
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • A Broad Range Of Species
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          393207

          References

          Reference overview
          Toyokuni, S., et al. 2000. Antioxid. Redox. Signal 2, 681.
          Neely, M.D., et al. 1999. J. Neurochem. 72, 2323.
          Uchida, K., et al. 1995. Biochem. Biophys. Res. Commun. 212, 1068.
          Uchida, K., et al. 1995. Arch. Biochem. Biophys. 324, 241.
          Uchida, K., et al. 1994. Biochemistry 33, 12487.

          Brochure

          Title
          Antibody Sourcebook!" Second Edition
          Nitric Oxide and Oxidative Stress Brochure
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision02-August-2007 RFH
          SynonymsAnti-4-Hydroxy-2-noneal Michael Adducts, Reduced
          ApplicationELISA (1:500-1:1000)
          Immunoblotting (1:1000-1:4000)
          Frozen Sections (1:50-1:500)
          Paraffin Sections (1:50-1:500)
          DescriptionRabbit polyclonal antibody supplied as diluted serum. Recognizes, specifically, chemically reduced amino acid-(4)-HNE adducts.
          BackgroundThe CNS is enriched in polyunsaturated fatty acids and is therefore particularly vulnerable to lipid peroxidation. 4-Hydroxy-2-noneal (HNE) is one of the major lipid peroxidation products with cytotoxic and mutagenic activity. It reacts with protein residues such as histidine to generate stable Michael adducts. HNE has been shown to inhibit neurite outgrowth and disrupt microtubules and tubulin modification, suggesting it may play a role in Alzheimer's disease.
          HostRabbit
          ImmunogenHNE-treated KLH in which 1:1 amino acid-HNE Michael adducts were chemically reduced
          IsotypeIgG
          Speciesa broad range of species
          FormLiquid
          FormulationSerum diluted in 50% glycerol, pH 7.5.
          PreservativeNone
          CommentsDoes not bind non-reduced adducts and reduced modifications arising from malondialdehyde (MDA) or structurally-related alkenals such as trans-2-noneal. Does not cross-react with non-reduced amino acid-HNE Michael adducts. Antibody binding requires the presence of the 4-hydroxyl group. Variables associated with assay conditions will dictate the optimal working dilution.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesToyokuni, S., et al. 2000. Antioxid. Redox. Signal 2, 681.
          Neely, M.D., et al. 1999. J. Neurochem. 72, 2323.
          Uchida, K., et al. 1995. Biochem. Biophys. Res. Commun. 212, 1068.
          Uchida, K., et al. 1995. Arch. Biochem. Biophys. 324, 241.
          Uchida, K., et al. 1994. Biochemistry 33, 12487.