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07-1586 | Anti-HIF-1-alpha Antibody, hydroxyproline (Pro564)

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, PIA Rb Affinity Purified Polyclonal Antibody
      Catalogue Number07-1586
      DescriptionAnti-HIF-1-alpha Antibody, hydroxyproline (Pro564)
      Alternate Names
      • Hypoxia-inducible factor 1-alpha
      • HIF-1-alpha
      • HIF1-alpha
      • ARNT-interacting protein
      • Basic-helix-loop-helix-PAS protein
      • MOP1
      • Class E basic helix-loop-helix protein 78
      • bHLHe78
      • Member of PAS protein 1
      • PAS domain-containing protein 8
      Background InformationCell growth and viability is compromised by oxygen deprivation (hypoxia). Hypoxia-inducible factors, including HIF-1α, HIF-1β (also designated Arnt 1), EPAS-1 (also designated HIF-2α) and HIF-3α, induce glycolysis, erythropoiesis and angiogenesis in order to restore oxygen homeostasis. Hypoxia-inducible factors are members of the Per-Arnt-Sim (PAS) domain transcription factor family. In response to hypoxia, HIF-1α is upregulated and forms a heterodimer with HIF-1β to form the HIF-1 complex. The HIF-1 complex recognizes and binds to the hypoxia responsive element (HRE) of hypoxia-inducible genes, thereby activating transcription. Hypoxia-inducible expression of some genes such as Glut-1, p53, p21 or Bcl-2, is HIF-1α dependent, whereas expression of others, such as p27, GADD 153 or HO-1, is HIF-1α independent. EPAS-1 and HIF-3α have also been shown to form heterodimeric complexes with Arnt 1 in response to hypoxia.
      Product Information
      FormatAffinity Purified
      • In vitro transcribed and translated HIF-1-alpha protein untreated and treated with FeCl2
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-HIF-1-alpha Antibody, hydroxyproline (Pro564) is a rabbit polyclonal antibody for detection of HIF-1-alpha also known as Hypoxia-inducible factor 1-alpha, HIF1-alpha & has been validated in WB, PIA.
      Key Applications
      • Western Blotting
      • Peptide Inhibition Assay
      Application NotesPeptide Inhibition Analysis: A representative lot of this antibody was used for peptide blocking using an in vitro transcribed and translated HIF-1-alpha protein.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminal VHL recognition site of human HIF-1-alpha, hydroxyproline (Pro564).
      EpitopeC-terminal VHL recognition site
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes the C-terminal VHL recognition site of HIF-1-alpha hydroxylated at Pro564.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
      Gene Symbol
      • HIF1A
      • BHLHE78
      • MOP1
      • PASD8
      • Hydroxylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP.

      SUBUNIT STRUCTURE: Interacts with the HIF1A beta/ARNT subunit; heterodimerization is required for DNA binding. Interacts with COPS5; the interaction increases the transcriptional activity of HIF1A through increased stability. Interacts with EP300 (via TAZ-type 1 domains); the interaction is stimulated in response to hypoxia and inhibited by CITED2. Interacts with CREBBP (via TAZ-type 1 domains). Interacts with NCOA1, NCOA2, APEX and HSP90. Interacts (hydroxylated within the ODD domain) with VHLL (via beta domain); the interaction, leads to polyubiquitination and subsequent HIF1A proteasomal degradation. During hypoxia, sumoylated HIF1A also binds VHL; the interaction promotes the ubiquitination of HIF1A. Interacts with SENP1; the interaction desumoylates HIF1A resulting in stabilization and activation of transcription. Interacts (Via the ODD domain) with ARD1A; the interaction appears not to acetylate HIF1A nor have any affect on protein stability, during hypoxia. Interacts with RWDD3; the interaction enhances HIF1A sumoylation. Interacts with TSGA10 By similarity. Interacts with RORA (via the DNA binding domain); the interaction enhances HIF1A transcription under hypoxia through increasing protein stability. Interaction with PSMA7 inhibits the transactivation activity of HIF1A under both normoxic and hypoxia-mimicking conditions. Interacts with USP20. Interacts with GNB2L1/RACK1; promotes HIF1A ubiquitination and proteasome-mediated degradation.

      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Cytoplasmic in normoxia, nuclear translocation in response to hypoxia. Colocalizes with SUMO1 in the nucleus, under hypoxia.

      TISSUE SPECIFICITY: Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors.
      Molecular Weight~92 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot using in vitro transcribed and translated HIF-1-alpha protein untreated and treated with FeCl2.

      Western Blot Analysis: A 1:1,000 dilution of this antibody detected in vitro transcribed and translated HIF-1-alpha protein treated with FeCl2.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-HIF-1-alpha, hydroxyproline (Pro564) - 2140078 2140078
      Anti-HIF-1-alpha, hydroxyproline (Pro564) - 2147117 2147117
      Anti-HIF-1-alpha, hydroxyproline (Pro564) - 2467135 2467135
      Anti-HIF-1-alpha, hydroxyproline (Pro564) - 2207235 2207235
      Anti-HIF-1-alpha, hydroxyproline (Pro564) - 2501945 2501945
      Anti-HIF-1-alpha, hydroxyproline (Pro564) - NRG1948879 NRG1948879
      Anti-HIF-1-alpha, hydroxyproline (Pro564) -2526907 2526907
      Anti-HIF-1-alpha, hydroxyproline (Pro564) -2537489 2537489
      Anti-HIF-1-alpha, hydroxyproline -2817721 2817721


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