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06-720 | Anti-HDAC1 Antibody

200 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M EA, ICC, IP, WB Rb Purified Polyclonal Antibody
      Catalogue Number06-720
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-HDAC1 Antibody
      Alternate Names
      • histone deacetylase 1, reduced potassium dependency, yeast homolog-like 1
      Background InformationMammalian HADC1 (also designated HD1), HDAC2 (also designated mammalian RPD3) and HDAC3, all are related to the yeast transcriptional regulator Rpd3p, are identified as histone deacetylases. The deacetylation of histones is associated with transcriptional silencing.
      Product Information
      • NIH/3T3 cell extract
        Included Positive Antigen Control: Catalog # 12-305, 3T3/A31 Cell Lysate. Add 2.5 μL of 2-mercaptoethanol per 100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
      PresentationPurified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, containing 0.05% sodium azide. Frozen at -20ºC.
      ApplicationUse Anti-HDAC1 Antibody (Rabbit Polyclonal Antibody) validated in EA, ICC, IP, WB to detect HDAC1 also known as histone deacetylase 1.
      Key Applications
      • Enzyme Assay
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      5 μg of a previous lot immunoprecipitated HDAC1 from 500 μg of 3T3/A31 RIPA lysate.

      This antibody has been reported to immunostain HDAC1 in 1% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized 3T3 cells.

      HDAC Activity:
      This antibody has been reported to immunoprecipitate active HDAC.
      Biological Information
      Immunogen6X-His-tagged fragment representing amino acids 53-482 of mouse histone deacetylase 1 (HDAC1).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes and is specific for HDAC1, Mr 65 kDa. May detects a non-specific protein at ~35 kDa.
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Species Reactivity NoteMouse, rat and human. Other species cross-reactivity not tested.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq]
      Gene Symbol
      • HDAC1
      • DKFZp686H12203
      • HD1
      • RPD3L1
      • GON-10
      • RPD3
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13547 # Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
      SIZE: 482 amino acids; 55103 Da
      SUBUNIT: Part of the core histone deacetylase (HDAC) complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core complex associates with MTA2, MBD2, MBD3, MTA1L1, CHD3 and CHD4 to form the nucleosome remodeling and histone deacetylation (NuRD) complex, or with SIN3, SAP18 and SAP30 to form the SIN3 HDAC complex. Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, AOF2/LSD1, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. Associates with the 9-1-1 complex; interacts with HUS1. Found in a complex with DNMT3A and HDAC7. Interacts with BCOR, BRMS1L, DAXX, DNMT1, EP300, HCFC1, PCAF, PHB2, MIER1, JMJD2A/JHDM3A, MINT, NRIP1, RERE, SETDB1, SUV39H1, TGIF, TGIF2, UHRF1 and UHRF2. Interacts with the non-histone region of H2AFY. Interacts with HDAC9. Component of a mSin3A corepressor complex that contains SIN3A, SAP130, SUDS3/SAP45, ARID4B/SAP180, HDAC1 and HDAC2. Interacts with BANP, CBFA2T3 and JARID1B. Interacts with SAP30L.
      TISSUE SPECIFICITY: Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
      PTM: Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1. & Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
      SIMILARITY: SwissProt: Q13547 ## Belongs to the histone deacetylase family. Type 1 subfamily.
      Molecular Weight65 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on RIPA lysates from 3T3/A31 cells.

      Western Blot Analysis:
      0.5-2 μg/mL of this lot detected HDAC1 in RIPA lysates from 3T3/A31 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations:
      Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-HDAC1 - 2387520 2387520
      Anti-HDAC1 - 2433482 2433482
      Anti-HDAC1 - 16360 16360
      Anti-HDAC1 - 18483 18483
      Anti-HDAC1 - 2020542 2020542
      Anti-HDAC1 - 20785 20785
      Anti-HDAC1 - 2197505 2197505
      Anti-HDAC1 - 23278 23278
      Anti-HDAC1 - 32643 32643
      Anti-HDAC1 - DAM1394791 DAM1394791
      Anti-HDAC1 - DAM1513999 DAM1513999
      Anti-HDAC1 - DAM1734763 DAM1734763
      Anti-HDAC1 - DAM1772421 DAM1772421
      Anti-HDAC1 - DAM1821113 DAM1821113
      Anti-HDAC1 - JBC1865953 JBC1865953
      Anti-HDAC1 -2571576 2571576
      Anti-HDAC1 -2710670 2710670
      Anti-HDAC1 -2739285 2739285

      References | 46 Available | See All References

      Reference overviewApplicationSpeciesPub Med ID
      The primary microRNA-208b interacts with Polycomb-group protein, Ezh2, to regulate gene expression in the heart.
      Mathiyalagan, P; Okabe, J; Chang, L; Su, Y; Du, XJ; El-Osta, A
      Nucleic acids research 42 790-803 2014

      Show Abstract
      24137001 24137001
      Oxidative stress and inflammation modulate Rev-erbα signaling in the neonatal lung and affect circadian rhythmicity.
      Yang, G; Wright, CJ; Hinson, MD; Fernando, AP; Sengupta, S; Biswas, C; La, P; Dennery, PA
      Antioxidants & redox signaling 21 17-32 2014

      Show Abstract
      24252172 24252172
      Corepressor-dependent silencing of fetal hemoglobin expression by BCL11A.
      Xu, J; Bauer, DE; Kerenyi, MA; Vo, TD; Hou, S; Hsu, YJ; Yao, H; Trowbridge, JJ; Mandel, G; Orkin, SH
      Proceedings of the National Academy of Sciences of the United States of America 110 6518-23 2013

      Show Abstract
      Human23576758 23576758
      Rad51 paralog complexes BCDX2 and CX3 act at different stages in the BRCA1-BRCA2-dependent homologous recombination pathway.
      Chun, J; Buechelmaier, ES; Powell, SN
      Molecular and cellular biology 33 387-95 2013

      Show Abstract
      23149936 23149936
      Deacetylation of p53 induces autophagy by suppressing Bmf expression.
      Contreras, AU; Mebratu, Y; Delgado, M; Montano, G; Hu, CA; Ryter, SW; Choi, AM; Lin, Y; Xiang, J; Chand, H; Tesfaigzi, Y
      The Journal of cell biology 201 427-37 2013

      Show Abstract
      23629966 23629966
      Identification of an intrinsic determinant critical for maspin subcellular localization and function.
      Dzinic, SH; Kaplun, A; Li, X; Bernardo, M; Meng, Y; Dean, I; Krass, D; Stemmer, P; Shin, N; Lonardo, F; Sheng, S
      PloS one 8 e74502 2013

      Show Abstract
      24278104 24278104
      Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development.
      Ma, P; Pan, H; Montgomery, RL; Olson, EN; Schultz, RM
      Proceedings of the National Academy of Sciences of the United States of America 109 E481-9 2012

      Show Abstract
      Immunofluorescence22223663 22223663
      IFN-α inhibits HBV transcription and replication in cell culture and in humanized mice by targeting the epigenetic regulation of the nuclear cccDNA minichromosome.
      Belloni, L; Allweiss, L; Guerrieri, F; Pediconi, N; Volz, T; Pollicino, T; Petersen, J; Raimondo, G; Dandri, M; Levrero, M
      The Journal of clinical investigation 122 529-37 2012

      Show Abstract
      22251702 22251702
      DNA methyltransferase 3b preferentially associates with condensed chromatin.
      Kashiwagi, K; Nimura, K; Ura, K; Kaneda, Y
      Nucleic acids research 39 874-88 2011

      Show Abstract
      Western Blotting20923784 20923784
      Polycomb eviction as a new distant enhancer function.
      Vernimmen, D; Lynch, MD; De Gobbi, M; Garrick, D; Sharpe, JA; Sloane-Stanley, JA; Smith, AJ; Higgs, DR
      Genes & development 25 1583-8 2011

      Show Abstract
      21828268 21828268

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      Included Positive Control

      Catalog Number Description  
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