A part of MilliporeSigma

MAB347 | Anti-Growth Associated Protein 43 Antibody, clone 9-1E12

50 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, Fe, Ht IHC, IP, WB M Purified Monoclonal Antibody
      Catalogue NumberMAB347
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Growth Associated Protein 43 Antibody, clone 9-1E12
      Alternate Names
      • Neuromodulin
      • B-50 Protein
      • Growth Associated Protein 43
      • neuromodulin
      Background InformationGAP-43, a common marker of differentiating neurons, is expressed at elevated levels by developing or regenerating neurons during axon growth. While GAP-43 is an integral membrane protein associated with the cytoplasmic surface of axonal growth cones (and synapses), it is absent from dendritic growth cones. In adult brain, GAP-43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus. Phosphorylation of GAP-43 by PKC (at Ser41 on human, mouse, rat, and bovine GAP-43 or Ser42 on chicken and Xenopus GAP-43) is specifically correlated with certain forms of synaptic plasticity.
      Product Information
      • Rat DRG tissue that has been subjected to a spinal nerve ligation and cultured neurons.
      PresentationProtein A Purified mouse immunoglobulin IgG1 in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
      ApplicationAnti-Growth Associated Protein 43 Antibody, clone 9-1E12 detects level of Growth Associated Protein 43 & has been published & validated for use in IH, IP & WB.
      Key Applications
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunohistochemistry:
      0.1-0.5 µg/mL of a previous lot on 4% paraformaldehyde perfused and fixed rat cerebellum and cerebrum tissue. The addition of 0.01-0.1% Triton X-100 to incubation buffer will increase cellular permeability.

      A previous lot of this antibody was used in immunoprecipitation.
      5-10µg/mL in membrane preparations from 16-18 day embryonic cortical neuronal cultures.

      Western Blot Analysis:
      MAB347 will detect a single 43-48 kD band in western blots of membrane fractions of growing neurons.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenGAP-43 purified from rat brain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityGAP-43 (also known as growth associated protein-43, B-50, F1 and pp46), regardless of the protein’s phosphorylation state.
      Species Reactivity
      • Human
      • Rat
      • Feline
      • Hamster
      Species Reactivity NoteRat, feline and hamster.
      Expected to react with human based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This encoded protein is considered a crucial component of an effective regenerative response in the nervous system.
      Gene Symbol
      • GAP43
      • PP46
      • B-50
      • GAP-43/B50
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P17677 # This protein is associated with nerve growth. It is a major component of the motile growth cones that form the tips of elongating axons.
      SIZE: 238 amino acids; 24803 Da
      SUBUNIT: Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence (By similarity).
      SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Note=Cytoplasmic surface of growth cone and synaptic plasma membranes.
      PTM: Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.
      SIMILARITY: SwissProt: P17677 ## Belongs to the neuromodulin family. & Contains 1 IQ domain.
      Molecular Weight43-48 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on rat brain lysates.

      Western Blot Analysis:
      1:1000 dilution of this lot detected GAP-43/B-50 on 10 μg of rat brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted for up to 6 months after date of receipt.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Growth Associated Protein 43, -2787663 2787663
      Anti-Growth Associated Protein 43, clone 9-1E12 - 2392312 2392312
      Anti-Growth Associated Protein 43, clone 9-1E12 - 2465201 2465201
      Anti-Growth Associated Protein 43, clone 9-1E12 - 2325120 2325120
      Anti-Growth Associated Protein 43, clone 9-1E12 - LV1786431 LV1786431
      Anti-Growth Associated Protein 43, clone 9-1E12 - NG1869850 NG1869850
      Anti-Growth Associated Protein 43, clone 9-1E12 -2512482 2512482
      Anti-Growth Associated Protein 43, clone 9-1E12 -2677789 2677789
      Anti-Growth Associated Protein 43, clone 9-1E12 -2710255 2710255
      Anti-Growth Associated Protein 43, clone 9-1E12 -2757138 2757138

      References | 62 Available | See All References

      Reference overviewApplicationSpeciesPub Med ID
      Gap43 transcription modulation in the adult brain depends on sensory activity and synaptic cooperation.
      Rosskothen-Kuhl, N; Illing, RB
      PloS one 9 e92624 2014

      Show Abstract
      24647228 24647228
      Developmentally dynamic colocalization patterns of DSCAM with adhesion and synaptic proteins in the mouse retina.
      de Andrade, GB; Kunzelman, L; Merrill, MM; Fuerst, PG
      Molecular vision 20 1422-33 2014

      Show Abstract
      25352748 25352748
      Up-regulation of GAP-43 in the chinchilla ventral cochlear nucleus after carboplatin-induced hearing loss: correlations with inner hair cell loss and outer hair cell loss.
      Kraus, KS; Ding, D; Jiang, H; Kermany, MH; Mitra, S; Salvi, RJ
      Hearing research 302 74-82 2013

      Show Abstract
      23707995 23707995
      Human conditionally immortalized neural stem cells improve locomotor function after spinal cord injury in the rat.
      Amemori, T; Romanyuk, N; Jendelova, P; Herynek, V; Turnovcova, K; Prochazka, P; Kapcalova, M; Cocks, G; Price, J; Sykova, E
      Stem cell research & therapy 4 68 2013

      Show Abstract
      23759119 23759119
      Chondroitinase enhances cortical map plasticity and increases functionally active sprouting axons after brain injury.
      Harris, NG; Nogueira, MS; Verley, DR; Sutton, RL
      Journal of neurotrauma 30 1257-69 2013

      Show Abstract
      23517225 23517225
      Proteoglycan abnormalities in olfactory epithelium tissue from subjects diagnosed with schizophrenia.
      Pantazopoulos, H; Boyer-Boiteau, A; Holbrook, EH; Jang, W; Hahn, CG; Arnold, SE; Berretta, S
      Schizophrenia research 150 366-72 2013

      Show Abstract
      24035561 24035561
      DISC1 variants 37W and 607F disrupt its nuclear targeting and regulatory role in ATF4-mediated transcription.
      Malavasi, EL; Ogawa, F; Porteous, DJ; Millar, JK
      Human molecular genetics 21 2779-92 2012

      Show Abstract
      22422769 22422769
      Differential effects of AdOx on gene expression in P19 embryonal carcinoma cells.
      Yan, L; Zhao, HY; Zhang, Y; Shen, YF
      BMC neuroscience 13 6 2012

      Show Abstract
      22221422 22221422
      The Na(+)/Ca(2+) exchanger NCKX4 governs termination and adaptation of the mammalian olfactory response.
      Stephan, AB; Tobochnik, S; Dibattista, M; Wall, CM; Reisert, J; Zhao, H
      Nature neuroscience 15 131-7 2012

      Show Abstract
      22057188 22057188
      Phosphorylation of adenylyl cyclase III at serine1076 does not attenuate olfactory response in mice.
      Cygnar, KD; Collins, SE; Ferguson, CH; Bodkin-Clarke, C; Zhao, H
      The Journal of neuroscience : the official journal of the Society for Neuroscience 32 14557-62 2012

      Show Abstract
      23077041 23077041

      Data Sheet

      Anti-Growth Associated Protein 43, clone 9-1E12 - Data Sheet

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      Life Science Research > Antibodies and Assays > Primary Antibodies