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MAB3070 | Anti-Granzyme B Antibody, clone GrB-7

MAB3070
250 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, IH(P) M Culture Supernatant Monoclonal Antibody
      Description
      Catalogue NumberMAB3070
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Granzyme B Antibody, clone GrB-7
      References
      Product Information
      FormatCulture Supernatant
      PresentationLiquid
      Applications
      ApplicationDetect Granzyme B using this Anti-Granzyme B Antibody, clone GrB-7 validated for use in WB, IH(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunoblotting

      Immunohistochemistry of granzyme B-expressing lymphocytes in sublimate sections and formalin-fixed paraffin-embedded tissues (1:20 dilution). Does not react with other cell types. Cannot be used on frozen sections.

      Note on the use of MAB3070 on paraffin sections:

      Tissue slides should be pretreated with an antigen retrieval method, such as treatment with 0.1M sodium citrate for 10 min at 100°C. A working dilution of 1:20 is advised, but optimal working dilutions must be determined by end user.
      Biological Information
      CloneGrB-7
      HostMouse
      SpecificityMonoclonal antibody recognizes the 33 kDa granzyme B.

      Reacts specifically with human serine protease granzyme B. Does not cross-react with human granzyme A. Granzyme B localizes in cytoplasmic granules and can be used as a marker for NK cells and activated cytotoxic T-cells.
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryCytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response.
      Gene Symbol
      • GZMB
      • GRB
      • CGL-1
      • CCPI
      • CSP-B
      • C11
      • CTLA1
      • CTLA-1
      • Granzyme-2
      • CSPB
      • SECT
      • Fragmentin-2
      • CGL1
      • HLP
      • CTSGL1
      • EC 3.4.21.79
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P10144 # This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
      SIZE: 247 amino acids; 27688 Da
      SUBCELLULAR LOCATION: Cytoplasmic granule. Note=Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.
      SIMILARITY: SwissProt: P10144 ## Belongs to the peptidase S1 family. Granzyme subfamily. & Contains 1 peptidase S1 domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C for up to 12 months.
      Packaging Information
      Material Size250 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-GRANZYME B MONOCLONAL ANTIBODY - 2127763 2127763
      MOUSE ANTI-GRANZYME B MONOCLONAL ANTIBODY - 2370660 2370660
      MOUSE ANTI-GRANZYME B -2512416 2512416
      MOUSE ANTI-GRANZYME B -2552334 2552334
      MOUSE ANTI-GRANZYME B -2583408 2583408
      MOUSE ANTI-GRANZYME B -2643589 2643589
      MOUSE ANTI-GRANZYME B -2689148 2689148
      MOUSE ANTI-GRANZYME B -2739493 2739493
      MOUSE ANTI-GRANZYME B -2788902 2788902
      MOUSE ANTI-GRANZYME B MONOCLONAL ANTIBODY - 2062062 2062062
      MOUSE ANTI-GRANZYME B MONOCLONAL ANTIBODY - 2190473 2190473
      MOUSE ANTI-GRANZYME B MONOCLONAL ANTIBODY - 2279478 2279478
      MOUSE ANTI-GRANZYME B MONOCLONAL ANTIBODY - 2361395 2361395

      References

      Reference overviewPub Med ID
      Regulatory, effector, and cytotoxic T cell profiles in long-term kidney transplant patients.
      Joanna Ashton-Chess,Emilie Dugast,Robert B Colvin,Magali Giral,Yohann Foucher,Anne Moreau,Karine Renaudin,Christophe Braud,Anne Devys,Sophie Brouard,Jean-Paul Soulillou
      Journal of the American Society of Nephrology : JASN 20 2009

      Show Abstract Full Text Article
      19357258 19357258
      Functional dissociation between proforms and mature forms of proteinase 3, azurocidin, and granzyme B in regulation of granulopoiesis.
      Stefan Sköld, Lennart Zeberg, Urban Gullberg, Tor Olofsson
      Experimental hematology 30 689-96 2002

      Show Abstract
      12135665 12135665
      Primary gastric apoptosis-rich T-cell lymphoma co-expressing CD4, CD8, and cytotoxic molecules.
      T F Barth, F Leithäuser, H Döhner, M Bentz, M Pawlita, U Schmid, P Möller
      Virchows Archiv : an international journal of pathology 436 357-64 2000

      Show Abstract
      10834539 10834539
      Granzyme B expression in Reed-Sternberg cells of Hodgkin's disease.
      Oudejans, J J, et al.
      Am. J. Pathol., 148: 233-40 (1996) 1996

      Show Abstract
      8546210 8546210
      Granzyme B-expressing peripheral T-cell lymphomas: neoplastic equivalents of activated cytotoxic T cells with preference for mucosa-associated lymphoid tissue localization.
      de Bruin, P C, et al.
      Blood, 84: 3785-91 (1994) 1994

      Show Abstract
      7524749 7524749

      Data Sheet

      Title
      Anti-Granzyme B, clone GrB-7 - Data Sheet