MAB374 Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5

MAB374
200 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ca, H, M, R, Rb, F, Fe, Po ELISA, IP, ICC, IF, IHC, WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB374
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5
      Alternate Names
      • glyceraldehyde 3-phosphate dehydrogenase
      • aging-associated gene 9 protein
      • glyceraldehyde-3-phosphate dehydrogenase
      • GAPDH
      • G3PDH
      • GAPD
      • OK/SW-cl.12
      • MGC88685
      • G3PD
      • EC 1.2.1.12
      Background InformationGlyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. As a 'house-keeping' enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic action in metabolism it is also involved in the initial stages of apoptosis or oxidative stress response where GAPDH is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH has also been found to bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders including the beta-amyloid precursor protein and the huntingtin protein where decreased function of GAPDH is associated with Alzheimer's and Huntington's disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP).
      References
      Product Information
      FormatPurified
      Control
      • It is expressed in all cells.
      PresentationPurified Mouse monoclonal IgG1 in PBS, pH 7.4 with 0.1% sodium azide.
      Applications
      ApplicationAnti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5 is a well published and extensively characterized monoclonal antibody. This purified mAb detects Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) & has been published & validated for use in ELISA, IP, IC, IF, IH & WB.
      Key Applications
      • ELISA
      • Immunoprecipitation
      • Immunocytochemistry
      • Immunofluorescence
      • Immunohistochemistry
      • Western Blotting
      Application NotesELISA:
      1:1,000,000 dilution of a previous lot ws used for Immunoaffinity purification of GAPDH.

      Immunocytochemistry:
      1:100 to 1:300 dilution of a previous lot was used in immunocytochemistry in PBS-BSA 10 mg/mL. 4% PFA, with 0.02% PBS-Triton X-100; 3 min RT (Griffoni, 2001).

      Western blot:
      1:100 to 1:300. Recognizes a 36kDa band of the reduced monomer. Non-reduced GAPDH runs as a 146kDa tetramer.

      Immunoaffinity purification of GAPDH.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenGlyceraldehyde-3-phosphate dehydrogenase from rabbit muscle.
      Clone6C5
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityGlyceraldehyde-3-phosphate dehydrogenase (GAPDH) from skeletal muscle. Antibody also recognizes cardiac GAPDH. GAPDH enzyme is detected in many non-muscle cells lines including HeLa, HCT-116 cells, U937 and THP-1 cells among others.
      IsotypeIgG1
      Species Reactivity
      • Canine
      • Human
      • Mouse
      • Rat
      • Rabbit
      • Fish
      • Feline
      • Pig
      Species Reactivity NoteHuman, porcine, canine, rat, mouse, rabbit, cat, and fish skeletal muscle.
      It has been reported that this antibody does not react with GAPDH from E. coli.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryGlyceraldehyde-3-phosphate dehydrogenase catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. A GAPD pseudogene has been mapped to Xp21-p11 and 15 GAPD-like loci have been identified.
      Gene Symbol
      • GAPDH
      • G3PDH
      • GAPD
      • G3PD
      Non-Reactive Species
      • E. coli
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P04406 # Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway.
      SIZE: 335 amino acids; 36053 Da
      SUBUNIT: Homotetramer. Binds PRKCI.
      SUBCELLULAR LOCATION: Cytoplasm. Note=Postnuclear and Perinuclear regions. Membrane.
      SIMILARITY: SwissProt: P04406 ## Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
      Molecular Weight38 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on A431 lysates.

      Western Blot Analysis:
      1:500 dilution of this antibody detected GLYCERALDEHYDE-3-PDH on 10 μg of A431 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8ºC in undiluted aliquots for up to 6 months after date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5 Certificates of Analysis

      TitleLot Number
      Anti-Glyceraldehyde-3-Phosphate -27929982792998
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C53075597
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C53053408
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 -
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - 21459252145925
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - 23888332388833
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - 24303172430317
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - 19908921990892
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - 22711442271144
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - 23225712322571

      References

      Reference overviewApplicationSpeciesPub Med ID
      Direct Exposure to Ethanol Disrupts Junctional Cell-Cell Contact and Hippo-YAP Signaling in HL-1 Murine Atrial Cardiomyocytes.
      Noritake, K; Aki, T; Funakoshi, T; Unuma, K; Uemura, K
      PloS one  10  e0136952  2015

      Show Abstract
      26317911 26317911
      Erythropoietin produced by genetic-modified NIH/3T3 fibroblasts enhances the survival of degenerating neurons.
      Li, YC; Chen, SJ; Chien, CL
      Brain and behavior  5  e00356  2015

      Show Abstract
      26357589 26357589
      A Glaucoma-Associated Variant of Optineurin, M98K, Activates Tbk1 to Enhance Autophagosome Formation and Retinal Cell Death Dependent on Ser177 Phosphorylation of Optineurin.
      Sirohi, K; Kumari, A; Radha, V; Swarup, G
      PloS one  10  e0138289  2015

      Show Abstract
      26376340 26376340
      Regulation of NKT cell-mediated immune responses to tumours and liver inflammation by mitochondrial PGAM5-Drp1 signalling.
      Kang, YJ; Bang, BR; Han, KH; Hong, L; Shim, EJ; Ma, J; Lerner, RA; Otsuka, M
      Nature communications  6  8371  2015

      Show Abstract
      26381214 26381214
      Prenatal ethanol exposure alters adult hippocampal VGLUT2 expression with concomitant changes in promoter DNA methylation, H3K4 trimethylation and miR-467b-5p levels.
      Zhang, CR; Ho, MF; Vega, MC; Burne, TH; Chong, S
      Epigenetics & chromatin  8  40  2015

      Show Abstract
      26421062 26421062
      Further Insights into the Allan-Herndon-Dudley Syndrome: Clinical and Functional Characterization of a Novel MCT8 Mutation.
      Armour, CM; Kersseboom, S; Yoon, G; Visser, TJ
      PloS one  10  e0139343  2015

      Show Abstract
      26426690 26426690
      Mitochondrial ROS Induces Cardiac Inflammation via a Pathway through mtDNA Damage in a Pneumonia-Related Sepsis Model.
      Yao, X; Carlson, D; Sun, Y; Ma, L; Wolf, SE; Minei, JP; Zang, QS
      PloS one  10  e0139416  2015

      Show Abstract
      Western Blotting26448624 26448624
      CSB interacts with SNM1A and promotes DNA interstrand crosslink processing.
      Iyama, T; Lee, SY; Berquist, BR; Gileadi, O; Bohr, VA; Seidman, MM; McHugh, PJ; Wilson, DM
      Nucleic acids research  43  247-58  2015

      Show Abstract
      25505141 25505141
      HMGB1 facilitates repair of mitochondrial DNA damage and extends the lifespan of mutant ataxin-1 knock-in mice.
      Ito, H; Fujita, K; Tagawa, K; Chen, X; Homma, H; Sasabe, T; Shimizu, J; Shimizu, S; Tamura, T; Muramatsu, S; Okazawa, H
      EMBO molecular medicine  7  78-101  2015

      Show Abstract
      25510912 25510912
      Milnacipran remediates impulsive deficits in rats with lesions of the ventromedial prefrontal cortex.
      Tsutsui-Kimura, I; Yoshida, T; Ohmura, Y; Izumi, T; Yoshioka, M
      The international journal of neuropsychopharmacology / official scientific journal of the Collegium Internationale Neuropsychopharmacologicum (CINP)  18  2015

      Show Abstract
      25522418 25522418

      Technical Info

      Title
      Protein Blotting Handbook: 6th Edition (MilliporeSigma)

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      Life Science Research > Antibodies and Assays > Primary Antibodies