A part of MilliporeSigma

ABS1651 | Anti-Glutathione S-Transferase A1/A2 Antibody

100 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
Request Pricing
Limited AvailabilityLimited Availability
Limited Quantities Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers


      Contact Customer Service

      Click To Print This Page


      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M WB Rb Serum Polyclonal Antibody
      Catalogue NumberABS1651
      DescriptionAnti-Glutathione S-Transferase A1/A2 Antibody
      Alternate Names
      • Glutathione S-transferase A1
      • Glutathione S-Transferase A1/A2
      • GST class-alpha member 1
      • Glutathione S-transferase Ya
      • Glutathione S-transferase Ya1
      • Glutathione S-transferase A1, N-terminally processed
      • Glutathione S-transferase A2
      • GST class-alpha member 2
      • Glutathione S-transferase GT41A
      Background InformationGlutathione S-transferase (GST) A1 (EC:; UniProt P13745; GST Ya, GST Ya1, GST A1, GST class-alpha member 1) and GST A2 (UniProt P10648; GT41A, GST Ya2, GST A2, GST class-alpha member 2) are encoded by the Gsta1 gene (Gene ID 14857) and the Gsta2 gene (Gene ID 14858), respectively, in murine species. Glutathione S-transferases constitute a family of metabolic isozymes that are involved in detoxification by catalyzing the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates. The GST family can be subdivided into the cytosolic, mitochondrial, and microsomal (a.k.a. MAPEG) proteins. Mammalian GSTs exist in either as homodimers or heterodimers. The individual monomers were historically characterized by their quaternary structure and size, Yf (Mr 24 500), Yk (25 kDa), Ya1 (25.5 kDa), Ya2 (25.5 kDa), Yn (26.5 kDa), Yb1 (27 kDa), Yb2 (27 kDa) and Yc (28.5 kDa). The “Y” designation refers to the “Y” fraction (as apposed to the “X and Z” fractions) they were found in during purification by Sephadex G75 chromatography. Murine Ya1 and Ya2 are different by only 11 out of their sequences (223 a.a./A1 or 223 a.a./A2). In addition, GST Ya1/Ya2 sequences are higly conserved among murine and rat species (>85% identity), while less sequence homology is found when comparing to the corresponding human proteins. Rat GST YaYa dimer was historically referred to as "Ligandin" or GST L, and old nomenclature also referred to human alpha classe GST A1-1, A1-2 and A2-2 as, respectively, B1B1, B1B2 and B2B2, where B stands for basic.
      Product Information
      PresentationRabbit polyclonal serum with 0.05% sodium azide.
      ApplicationAnti-Glutathione S-Transferase A1/A2 Antibody is an antibody against Glutathione S-Transferase A1/A2 for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:5,000 dilution from a representative lot detected Glutathione S-Transferase A1/A2 in 10 µg of mouse testis and small intestine tissue lysate.
      Western Blotting Analysis: A representative lot detected upregulated Glutathione S-transferase A1 & A2 (GSTA1/A2) in liver cytosolic preparations from mice on high-fat diet (HF) when compared with liver samples from mice on regular diet (Meakin, P.J., et al. (2014). Mol. Cell. Biol. 34(17):3305-3320).
      Western Blotting Analysis: A representative lot detected anticarcinogen-induced Glutathione S-transferase A1 & A2 (GSTA1/A2) expression in liver cytosolic preparations from C57BL/6 mice received intraperitoneal beta-naphthoflavone (BNF) injection, while such induction was not observed in mouse strain DBA/2 without functional aryl hydrocarbon (Ah) receptor (McLellan, L.I., et al. (1991). Biochem. J. 276(Pt 2):461-469).
      Western Blotting Analysis: A representative lot detected antioxidant-induced Glutathione S-transferase A1 & A2 (GSTA1/A2) expression in liver cytosolic preparations from mice fed with dietary 2(3)-t-butyl-4-hydroxyanisole (BHA) supplementation when compared with liver samples from mice without BHA supplementation (McLellan, L.I., and Hayes, J.D. (1989). Biochem. J. 263(2):393-402).
      Biological Information
      ImmunogenPurified mouse liver Glutathione S-Transferase A1/A2.
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityThis antiserum cannot distinguish between Glutathione S-transferase A1 and A2, which share more than 95% sequence homology (UniProt P13745 & P10648).
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. Predicted to react with Rat based on high sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Gsta1
      • Gsta
      • Gstya
      • Gsta2
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~25 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse stomach tissue lysate.

      Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Glutathione S-Transferase A1/A2 in 10 µg of mouse stomach tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information


      Certificates of Analysis

      TitleLot Number
      Anti-Glutathione S-Transferase A1/A2 -2659597 2659597
      Anti-Glutathione S-Transferase A1/A2 -Q2618555 Q2618555


      Reference overviewPub Med ID
      Susceptibility of Nrf2-null mice to steatohepatitis and cirrhosis upon consumption of a high-fat diet is associated with oxidative stress, perturbation of the unfolded protein response, and disturbance in the expression of metabolic enzymes but not with insulin resistance.
      Meakin, PJ; Chowdhry, S; Sharma, RS; Ashford, FB; Walsh, SV; McCrimmon, RJ; Dinkova-Kostova, AT; Dillon, JF; Hayes, JD; Ashford, ML
      Molecular and cellular biology 34 3305-20 2014

      Show Abstract
      24958099 24958099
      Regulation of mouse glutathione S-transferases by chemoprotectors. Molecular evidence for the existence of three distinct alpha-class glutathione S-transferase subunits, Ya1, Ya2, and Ya3, in mouse liver.
      McLellan, LI; Kerr, LA; Cronshaw, AD; Hayes, JD
      The Biochemical journal 276 ( Pt 2) 461-9 1991

      Show Abstract
      2049074 2049074
      Differential induction of class alpha glutathione S-transferases in mouse liver by the anticarcinogenic antioxidant butylated hydroxyanisole. Purification and characterization of glutathione S-transferase Ya1Ya1.
      McLellan, LI; Hayes, JD
      The Biochemical journal 263 393-402 1989

      Show Abstract
      2597111 2597111


      New Products Flyer- Vol 4 Signaling Feature- NEW! Phosphohistidine Antibody (EMD)


      Post-Translational Modifications