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AB5010 | Anti-Glutathione Antibody, detects both GSH & GSSG

500 µL  sufficient for 2000 assays
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Eu IHC Rb Purified Polyclonal Antibody
      Catalogue NumberAB5010
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Glutathione Antibody, detects both GSH & GSSG
      Product Information
      PresentationPurified immunoglobulin. Liquid in stabilizing buffer (Phosphate buffer with sterile goat serum) containing 0.05% thimerosal.
      ApplicationThis Anti-Glutathione Antibody, detects both GSH & GSSG is validated for use in IH for the detection of Glutathione.
      Key Applications
      • Immunohistochemistry
      Application NotesImmunohistochemistry using silver-intensified immunogold or enhanced fluorescence methods. Treatment with autofluorescent reducers is typically necessary when using glutaraldehyde fixatives. Samples should be fixed with 0.2% - 2.5% glutaraldehyde for optimum detection. See notes technical notes. This antibody has also been used and found to work with a zero-low glutaraldehyde / high paraformaldehyde fixation (4% paraformaldehyde in 0.1M phosphate buffer / 3% sucrose fixative ). The minimum glutaraldehyde concentration for AB5010 is 0.02%. Performance is good with frozen sections, Vibratome sections and tissue culture formats, when penetrating reagents such as 0.3% Triton X-100 are used (only in blocking buffer, antibody dilution buffer should be in simple TBS or PBS only).

      Western blot (Mawatari and Murakami, 2004).

      Optimal working dilutions must be determined by the end user.

      Note: Glutathione is not osmium tolerant and therefore should not be used for post-embedding electron micoscope immunohistochemistry.

      All procedures may be carried out at room temperature. Though the exact dilutions for all applications can not be predicated, it is unlikely that deviations from the calibrated concentration will be needed. The 100X dilution is calibrated to optimize the detection of antigen levels over a 2 log unit range. Greater or lesser dilutions may be employed if unusually high (>20 mM) or low (<0.2 mM) intracellular hapten levels are anticipated or novel specimen conditions exist, such as low glutaraldehyde levels or unusual embedding protocols. Greater dilutions will likely result in decreased maximum signal strength whereas more concentrated solutions will compress the dynamic range of detection.


      The AB5010 is optimized for use with immunogold or fluorescence detection. Visualization with enzyme-linked methods can be used but they typically compress the dynamic range of detection. Use of high gain methods such as tyramides will require the user to perform independent calibrations. Use with frozen sections is possible but will not yield optimal images as IgGs will penetrate aldehyde cross-linked tissue poorly and most amino acids are present at such high levels that prozone effect occurs. Use in whole mount or vibratome sections is not recommended for similar reasons.
      Biological Information
      Epitopedetects both GSH & GSSG
      SpecificityGlutathione. The antibody has been calibrated against a spectrum of antigens to assure hapten selectivity and proper affinity. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) against L-alanine, g-aminobutyrate, 1-amino-4-guanidobutane (AGB), D/L-arganine, D/L-aspartate, L-citrulline, L-cysteine, D/L-glutamate, D/L-glutamine, glycine, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine. Does not discriminate between free GSH and GSSG.
      Species Reactivity
      • Eukaryote
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain stock at 2-8°C in undiluted aliquots for up to 6 months. This stock is extremely stable under normal use and routine storage at 2-8°C. Do not refreeze this stock.
      Packaging Information
      Material Size500 µL
      Material Packagesufficient for 2000 assays
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-GLUTATHIONE -2742743 2742743
      RABBIT ANTI-GLUTATHIONE -2800213 2800213


      Reference overviewApplicationPub Med ID
      Neocortex and allocortex respond differentially to cellular stress in vitro and aging in vivo.
      Posimo, JM; Titler, AM; Choi, HJ; Unnithan, AS; Leak, RK
      PloS one 8 e58596 2013

      Show Abstract
      Western Blotting23536801 23536801
      Astrocyte plasticity revealed by adaptations to severe proteotoxic stress.
      Titler, Amanda M, et al.
      Cell Tissue Res., 352: 427-43 (2013) 2013

      Show Abstract
      23420451 23420451
      Rescue from a two hit, high-throughput model of neurodegeneration with N-acetyl cysteine.
      Ajay S Unnithan,Hailey J H Choi,Amanda M Titler,Jessica M Posimo,Rehana K Leak
      Neurochemistry international 61 2012

      Show Abstract
      22691629 22691629
      Single-cell redox imaging demonstrates a distinctive response of dopaminergic neurons to oxidative insults.
      Horowitz, MP; Milanese, C; Di Maio, R; Hu, X; Montero, LM; Sanders, LH; Tapias, V; Sepe, S; van Cappellen, WA; Burton, EA; Greenamyre, JT; Mastroberardino, PG
      Antioxidants & redox signaling 15 855-71 2011

      Show Abstract
      21395478 21395478
      Subcellular immunocytochemical analysis detects the highest concentrations of glutathione in mitochondria and not in plastids.
      Zechmann, B, et al.
      J. Exp. Bot., 59: 4017-27 (2008) 2008

      Show Abstract
      18977750 18977750
      Intracellular adaptations of glutathione content in Cucurbita pepo L. induced by treatment with reduced glutathione and buthionine sulfoximine.
      Zechmann, B, et al.
      Protoplasma, 227: 197-209 (2006) 2006

      Show Abstract
      16520878 16520878
      Changes in the subcellular distribution of glutathione during virus infection in Cucurbita pepo (L.).
      Zechmann, B, et al.
      Plant Biol (Stuttg), 7: 49-57 (2005) 2005

      Show Abstract
      15666214 15666214
      Temperature effects on the MgATP-induced electron transfer between the nitrogenase proteins from Azotobacter vinelandii.
      Mensink, R E and Haaker, H
      Eur. J. Biochem., 208: 295-9 (1992) 1992

      Show Abstract
      1521527 1521527


      Pathways and Biomarkers of Oxidative Stress (EMD)

      Technical Info

      White Paper- Modern Methods in Oxidative Stress Research (EMD)

      Data Sheet

      Anti-Glutathione, detects both GSH & GSSG - Data Sheet