AB1504 Anti-Glutamate receptor 1 Antibody

AB1504
200 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, M, H, Eq WB, IH(P), IP Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberAB1504
      DescriptionAnti-Glutamate receptor 1 Antibody
      Alternate Names
      • AMPA 1
      • AMPA-selective glutamate receptor 1
      • Glutamate receptor ionotropic, AMPA 1
      • glutamate receptor, ionotropic, AMPA 1
      Background InformationGlutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcatergorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. GluR1 is believed to mediate calcium influx, and has a vital function in synaptic plasticity related to learning. Reduction in GluR1 has been caused by FMR1 gene mutation and contributes to fragile X syndrome. Changes in expression of GluR1 receptors on dopamine neurons have been correlated with sensitization of drugs of abuse.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Mouse brain lysate
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-Glutamate receptor 1 Antibody is validated for use in WB, IH(P), IP for the detection of Glutamate receptor 1.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      Application NotesImmunohistochemistry Analysis: 1:300 dilution of this antibody has been shown to detect Glutamate receptor 1 (GluR1) in rat brain tissue.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Glutamate receptor 1 at the cytoplasmic domain.
      EpitopeCytoplasmic domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes Glutamate receptor 1 at the cytoplasmic domain.
      Species Reactivity
      • Rat
      • Mouse
      • Human
      • Horse
      Species Reactivity NoteDemonstrated to react with human, mouse, and rat. Predicted to react with equine and platypus based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryGlutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
      Gene Symbol
      • GRIA1
      • GluR-K1
      • GluR-A
      • GLUH1
      • GLUR1
      • GLURA
      • HBGR1
      • GluR-1
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.

      >SIZE: 906 amino acids; 101506 Da

      SUBUNIT: Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with DLG1 via its C-terminus. Interacts with CACNG2 and RASGRF2 (By similarity).

      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Note=Interaction with CACNG2 promotes cell surface expression (By similarity).

      TISSUE SPECIFICITY: Widely expressed in brain.

      PTM: Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity).

      SIMILARITY: Belongs to the glutamate-gated ion channel (TC 1.A.10) family.

      MISCELLANEOUS: The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate.
      Molecular WeightObserved at ~106 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in mouse brain lysate.

      Western Blot Analysis: 1:1000 dilution of this antibody detected Glutamate receptor 1 (GluR1) on 10 µg of mouse brain lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-GluR1 - LV1446043ALV1446043A
      Anti-GluR1 - LV1519215LV1519215
      Anti-GluR1 - LV1563305LV1563305
      Anti-GluR1 - LV1619032LV1619032
      Anti-GluR1 - LV1620620LV1620620
      Anti-GluR1 - LV1623825LV1623825
      Anti-GluR1 - LV1726018LV1726018
      Anti-Glutamate Receptor 1 - 32023933202393
      Anti-Glutamate Receptor 1 -26341852634185
      Anti-Glutamate receptor 1 - 21217632121763

      References

      Reference overviewApplicationSpeciesPub Med ID
      Cellular plasticity induced by anti-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor encephalitis antibodies.
      Peng, X; Hughes, EG; Moscato, EH; Parsons, TD; Dalmau, J; Balice-Gordon, RJ
      Annals of neurology  77  381-98  2015

      Show Abstract
      25369168 25369168
      Postsynaptic VAMP/Synaptobrevin Facilitates Differential Vesicle Trafficking of GluA1 and GluA2 AMPA Receptor Subunits.
      Hussain, S; Davanger, S
      PloS one  10  e0140868  2015

      Show Abstract
      26488171 26488171
      A negative feedback loop controls NMDA receptor function in cortical interneurons via neuregulin 2/ErbB4 signalling.
      Vullhorst, D; Mitchell, RM; Keating, C; Roychowdhury, S; Karavanova, I; Tao-Cheng, JH; Buonanno, A
      Nature communications  6  7222  2015

      Show Abstract
      26027736 26027736
      AMPA Receptor-mTOR Activation is Required for the Antidepressant-Like Effects of Sarcosine during the Forced Swim Test in Rats: Insertion of AMPA Receptor may Play a Role.
      Chen, KT; Tsai, MH; Wu, CH; Jou, MJ; Wei, IH; Huang, CC
      Frontiers in behavioral neuroscience  9  162  2015

      Show Abstract
      26150775 26150775
      Synaptic and cognitive improvements by inhibition of 2-AG metabolism are through upregulation of microRNA-188-3p in a mouse model of Alzheimer's disease.
      Zhang, J; Hu, M; Teng, Z; Tang, YP; Chen, C
      The Journal of neuroscience : the official journal of the Society for Neuroscience  34  14919-33  2014

      Show Abstract
      25378159 25378159
      Simvastatin treatment enhances NMDAR-mediated synaptic transmission by upregulating the surface distribution of the GluN2B subunit.
      Parent, MA; Hottman, DA; Cheng, S; Zhang, W; McMahon, LL; Yuan, LL; Li, L
      Cellular and molecular neurobiology  34  693-705  2014

      Show Abstract
      24687455 24687455
      Synapse elimination and learning rules co-regulated by MHC class I H2-Db.
      Lee, H; Brott, BK; Kirkby, LA; Adelson, JD; Cheng, S; Feller, MB; Datwani, A; Shatz, CJ
      Nature  509  195-200  2014

      Show Abstract
      Western Blotting24695230 24695230
      Antigenic and mechanistic characterization of anti-AMPA receptor encephalitis.
      Gleichman, AJ; Panzer, JA; Baumann, BH; Dalmau, J; Lynch, DR
      Annals of clinical and translational neurology  1  180-189  2014

      Show Abstract
      24707504 24707504
      Evolutionarily conserved pattern of AMPA receptor subunit glycosylation in Mammalian frontal cortex.
      Tucholski, J; Pinner, AL; Simmons, MS; Meador-Woodruff, JH
      PloS one  9  e94255  2014

      Show Abstract
      Western Blotting24713873 24713873
      MET receptor tyrosine kinase controls dendritic complexity, spine morphogenesis, and glutamatergic synapse maturation in the hippocampus.
      Qiu, S; Lu, Z; Levitt, P
      The Journal of neuroscience : the official journal of the Society for Neuroscience  34  16166-79  2014

      Show Abstract
      Immunohistochemistry25471559 25471559

      Brochure

      Title
      Pathways and Biomarkers of Glutamatergic Synapse Flyer (EMD)

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies