AB1783 Anti-Glutamate Transporter Antibody, Glial

AB1783
50 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R IH(P), IF, WB Gp Serum Polyclonal Antibody
      Description
      Catalogue NumberAB1783
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Glutamate Transporter Antibody, Glial
      Alternate Names
      • GLT-1
      • EAAT2
      Background InformationGlutamate transporters (GluT) function to remove L-glutamate (Glu), the primary excitatory neurotransmitter in the mammalian central nervous system (CNS), from the synaptic cleft. By clearing the synapse in this manner Glu can be recycled for later use, the proper diffusion gradient can be maintained and excitotoxicity can be prevented. There have been reports of many different members of a GluT multigene family; GLT1, GLAST, EAAC1 and EAAT2. mRNA has been identified in brain for each of these proteins.

      EAAT2 transports L-glutamate and also L- and D-aspartate. It is essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
      References
      Product Information
      FormatSerum
      Control
      • Rat brain tissue
      PresentationGuinea pig antiserum containing no preservatives.
      Applications
      ApplicationDetect Glutamate Transporter using this Anti-Glutamate Transporter Antibody, Glial validated for use in IH(P), IF & WB.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunofluorescence
      • Western Blotting
      Application NotesEvaluated by Western Blot on mouse brain membrane lysates.

      Western Blot: 1:500 dilution of this antibody detected GLT-1 on 10 ug of mouse brain membrane lysates.

      Immunohistochemistry:
      1:1,000-1:4,000 dilution from a previuos lot used a DAB detection system on adult rat forebrain fixed with 4% paraformaldehyde.

      Immunohistochemistry:
      1:5,000-10,000 dilution from a previuos lot used a cyanine conjugated secondary antibody.

      Enzymatic detection requires substantially higher primary antibody dilutions. Lightly fixed 4% PFA material is recommended.

      Notes:

      Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with 50 mL of Ca2+-free Tyrode+s solution followed by a formalin-picric acid fixative (4% paraformaldehyde with 0.4% picric acid in 0.16 M phosphate buffer, pH 6.9) for 6 minutes. Tissues were rapidly dissected out, postfixed in the same fixative for 90 minutes and rinsed for at least 24 hours in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Sections were cut (14 um) in a cryostat and incubated at 4°C overnight with AB1783 (1:5,000-1:10,000). After rinsing in PBS sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.



      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic peptide from the carboxy-terminus of rat GLT-1.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostGuinea Pig
      SpecificityGlial Glutamate Transporter GLT-1 (EAAT2). The antibody has been tested on central nervous system tissue. Preabsorption of the antiserum with the immunogen peptide (Catalog number AG391) completely abolishes the immunostaining.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
      Gene Symbol
      • SLC1A2
      • GLT-1
      • EAAT2
      • GLT1
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P43004 # Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
      SIZE: 574 amino acids; 62104 Da
      SUBUNIT: Homotrimer.
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      PTM: Glycosylated.
      SIMILARITY: SwissProt: P43004 ## Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family.
      Molecular Weight~62 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on mouse brain membrane lysates.

      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Glutamate Transporter, Glial - 21180142118014
      Anti-Glutamate Transporter, Glial - 23847292384729
      Anti-Glutamate Transporter, Glial - 24595922459592
      Anti-Glutamate Transporter, Glial - 19608541960854
      Anti-Glutamate Transporter, Glial - 20159782015978
      Anti-Glutamate Transporter, Glial - 21125312112531
      Anti-Glutamate Transporter, Glial - 21960292196029
      Anti-Glutamate Transporter, Glial - 22341552234155
      Anti-Glutamate Transporter, Glial - 24890692489069
      Anti-Glutamate Transporter, Glial - LV1547805LV1547805

      References

      Reference overviewApplicationSpeciesPub Med ID
      Buyang Huanwu decoction increases the expression of glutamate transporter-1 and glutamate synthetase in association with PACAP-38 following focal ischemia.
      Ding, W; Yu, P; Liu, W; Zhou, L; Guan, LI; Lin, R
      Biomedical reports  3  651-656  2015

      Show Abstract
      26405540 26405540
      Decreased EAAT2 protein expression in the essential tremor cerebellar cortex.
      Lee, M; Cheng, MM; Lin, CY; Louis, ED; Faust, PL; Kuo, SH
      Acta neuropathologica communications  2  157  2014

      Show Abstract
      Western Blotting, Immunohistochemistry25391854 25391854
      Novel treatment with neuroprotective and antiviral properties against a neuroinvasive human respiratory virus.
      Brison, E; Jacomy, H; Desforges, M; Talbot, PJ
      Journal of virology  88  1548-63  2014

      Show Abstract
      24227863 24227863
      Activity of D-amino acid oxidase is widespread in the human central nervous system.
      Sasabe, J; Suzuki, M; Imanishi, N; Aiso, S
      Frontiers in synaptic neuroscience  6  14  2014

      Show Abstract
      24959138 24959138
      Localization of excitatory amino acid transporters EAAT1 and EAAT2 in human postmortem cortex: a light and electron microscopic study.
      Roberts, RC; Roche, JK; McCullumsmith, RE
      Neuroscience  277  522-40  2014

      Show Abstract
      25064059 25064059
      Astrocytosis in parkinsonism: considering tripartite striatal synapses in physiopathology?
      Charron, G; Doudnikoff, E; Canron, MH; Li, Q; Véga, C; Marais, S; Baufreton, J; Vital, A; Oliet, SH; Bezard, E
      Frontiers in aging neuroscience  6  258  2014

      Show Abstract
      Immunohistochemistry25309435 25309435
      Cxcl12/Cxcr4 signaling controls the migration and process orientation of A9-A10 dopaminergic neurons.
      Yang, S; Edman, LC; Sánchez-Alcañiz, JA; Fritz, N; Bonilla, S; Hecht, J; Uhlén, P; Pleasure, SJ; Villaescusa, JC; Marín, O; Arenas, E
      Development (Cambridge, England)  140  4554-64  2013

      Show Abstract
      Immunohistochemistry24154522 24154522
      Unique features of extracellular matrix in the mouse medial nucleus of trapezoid body - Implications for physiological functions.
      Blosa, M, et al.
      Neuroscience, 228: 215-34 (2013)  2013

      Show Abstract
      23069754 23069754
      Lesion-induced alterations in astrocyte glutamate transporter expression and function in the hippocampus.
      Schreiner, AE; Berlinger, E; Langer, J; Kafitz, KW; Rose, CR
      ISRN neurology  2013  893605  2013

      Show Abstract
      24078881 24078881
      Mislocalization of the exitatory amino-acid transporters (EAATs) in human astrocytoma and non-astrocytoma cancer cells: effect of the cell confluence.
      Varini, K; Benzaria, A; Taïeb, N; Di Scala, C; Azmi, A; Graoudi, S; Maresca, M
      Journal of biomedical science  19  10  2012

      Show Abstract
      22296701 22296701

      Brochure

      Title
      Pathways and Biomarkers of Glutamatergic Synapse Flyer (EMD)

      Data Sheet

      Title
      Anti-Glutamate Transporter, Glial - Data Sheet

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      Categories

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