MAB397 Anti-Glutamate Receptor 2 Antibody, extracellular, clone 6C4

MAB397
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, Mk, Ca ELISA, ICC, IHC, IP, RIA, WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB397
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Glutamate Receptor 2 Antibody, extracellular, clone 6C4
      Alternate Names
      • GluR2
      Background InformationGlutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcatergorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. Although subunit composition of AMPA receptors varies, they must contain at least one edited GluR2 subunit to be calcium impermeable. The critical residue controlling calcium permeability is in the pore loop region. In GluR1, GluR3, and GluR4, this positionis occupied by a Gln residue. In GluR2, it is occupied by an Arg residue. It has been shown experimentally that the presence of Arg in this position blocks CA2+ ion permeability, while a Gln does not. Relative calcium permeability in AMPA receptor channels may be significant in pathological neurotoxic damage and long term changes in nervous system responses.
      References
      Product Information
      FormatPurified
      Control
      • Positive Control: Cerebral cortex and pyramidal neurons, mouse brain lysate.
      PresentationPurified mouse immunoglobin IgG2a liquid in buffer containing PBS, no preservative.
      Applications
      ApplicationAnti-Glutamate Receptor 2 Antibody, extracellular, clone 6C4 detects level of Glutamate Receptor 2 & has been published & validated for use in ELISA, IC, IH, IP, RIA & WB with more than 50 product citations.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      • Radioimmunoassay
      • Western Blotting
      Application NotesImmunocytochemistry:
      on 4% paraformaldehyde fixed cells was used in a previous lot:2-3 µg/mL (Vissavajjhala, 1996; Osten, 1998; Passafaro, 2001).

      ELISA/RIA:
      A previous lot of this antibody was used in ELISA/RIA.(Vissavajjhala, 1996).

      Immunohistochemistry on 50 µm, 4% paraformaldehyde fixed brain sections: 1:500-1:800 (Vissavajjhala, 1996; Yung, 1998; Kumar; 2002).

      Immunoprecipitation: 2-4 µg/mL (Osten, 1998).

      Western blot: 1-2 µg/mL (Vissavajjhala, 1996; Osten, 1998); membrane preparations are suggested for enhanced signals.

      Optimal working dilutions and protocols must be determined by end user.
      Biological Information
      ImmunogenRecombinant fusion protein TrpE-GluR2 (putative N-terminal portion, amino acids 175-430).
      EpitopeExtracellular
      Clone6C4
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes the large N-terminal extracellular domain of Glutamate Receptor 2 (GluR2). No cross-reactivity observed with other AMPA/Kainate GluR subunits. On western blots of brain extracts from rat, macaque monkey, and dog, MAB397 recognizes a band at approximately 102 kDa corresponding to full length GluR2. Other proteins noted by Western blot at 66 kDa or lower molecular weight appear to be breakdown products of GluR2 (Vissavajjhala, 1996). By immunohistochemistry GluR2 is widely distributed at both the cellular and synaptic levels. MAB397 recognizes GluR2 present in a vast majority of, but not all, GABAergic interneurons (Vissavajjhala, 1996).
      IsotypeIgG2a
      Species Reactivity
      • Mouse
      • Rat
      • Monkey
      • Canine
      Species Reactivity NoteExpected to react with rat, mouse and canine based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryGlutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, Gria1-4. The subunit encoded by this gene (Gria2) is subject to RNA editing (Q/R and R/G), which is thought to render the channels impermeable to Ca(2+), and to affect the kinetic aspects of these channels in rat brain. Alternative splicing, resulting in transcript variants encoding different isoforms (flip and flop), has been noted for this gene.
      Gene Symbol
      • Gria2
      • GLUR2
      • GLURB
      • GluR-2
      • GluR-B
      • GluR-K2
      Purification MethodPurified immunoglobulin from culture supernatant
      UniProt Number
      UniProt SummaryFUNCTION: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
      SIZE: 883 amino acids; 98,688 Da
      SUBUNIT: Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. May interact with MPP4. Forms a ternary complex with GRIP1 and CSPG4(By similarity). Interacts with CACNG2, PRKCABP, GRIP1 and GRIP2. Interacts with NSF via its C-terminus. Part of a complex containing GRIA2, NSF and NAPA and/or NAPB.
      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Note= Interaction with CACNG2 promotes cell surface expression.
      TISSUE SPECIFICITY: Detected in forebrain. Detected in dendrites of neuronal cells.
      SIMILARITY: Belongs to the glutamate-gated ion channel (TC 1.A.10) family. ...
      Molecular Weight102 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on mouse brain lysates.

      Western Blot Analysis:
      1:1000 dilution of this lot detected glutamate receptor 2 on 10 μg of mouse brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at -20ºC in undiluted aliquots from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage the IgG2a and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Glutamate Receptor 2, -25521212552121
      Anti-Glutamate Receptor 2, -28028122802812
      Anti-Glutamate Receptor 2, extracellular, clone 6 Monoclonal Antibody2930461
      Anti-Glutamate Receptor 2, extracellular, clone 6C42474862
      Anti-Glutamate Receptor 2, extracellular, clone 6C43073828
      Anti-Glutamate Receptor 2, extracellular, clone 6C4 - 21365772136577
      Anti-Glutamate Receptor 2, extracellular, clone 6C4 - 24265292426529
      Anti-Glutamate Receptor 2, extracellular, clone 6C4 - 19763831976383
      Anti-Glutamate Receptor 2, extracellular, clone 6C4 - 20021752002175
      Anti-Glutamate Receptor 2, extracellular, clone 6C4 - 20492092049209

      References

      Reference overviewApplicationSpeciesPub Med ID
      Cellular plasticity induced by anti-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor encephalitis antibodies.
      Peng, X; Hughes, EG; Moscato, EH; Parsons, TD; Dalmau, J; Balice-Gordon, RJ
      Annals of neurology  77  381-98  2015

      Show Abstract
      25369168 25369168
      Long-term potentiation decay and memory loss are mediated by AMPAR endocytosis.
      Dong, Z; Han, H; Li, H; Bai, Y; Wang, W; Tu, M; Peng, Y; Zhou, L; He, W; Wu, X; Tan, T; Liu, M; Wu, X; Zhou, W; Jin, W; Zhang, S; Sacktor, TC; Li, T; Song, W; Wang, YT
      The Journal of clinical investigation  125  234-47  2015

      Show Abstract
      25437879 25437879
      Diversity of glutamatergic synaptic strength in lateral prefrontal versus primary visual cortices in the rhesus monkey.
      Medalla, M; Luebke, JI
      The Journal of neuroscience : the official journal of the Society for Neuroscience  35  112-27  2015

      Show Abstract
      25568107 25568107
      Protein tyrosine phosphatase receptor type R is required for Purkinje cell responsiveness in cerebellar long-term depression.
      Erkens, M; Tanaka-Yamamoto, K; Cheron, G; Márquez-Ruiz, J; Prigogine, C; Schepens, JT; Nadif Kasri, N; Augustine, GJ; Hendriks, WJ
      Molecular brain  8  1  2015

      Show Abstract
      25571783 25571783
      The intellectual disability protein RAB39B selectively regulates GluA2 trafficking to determine synaptic AMPAR composition.
      Mignogna, ML; Giannandrea, M; Gurgone, A; Fanelli, F; Raimondi, F; Mapelli, L; Bassani, S; Fang, H; Van Anken, E; Alessio, M; Passafaro, M; Gatti, S; Esteban, JA; Huganir, R; D'Adamo, P
      Nature communications  6  6504  2015

      Show Abstract
      Western Blotting25784538 25784538
      In vivo generation of immature inner hair cells in neonatal mouse cochleae by ectopic Atoh1 expression.
      Liu, Z; Fang, J; Dearman, J; Zhang, L; Zuo, J
      PloS one  9  e89377  2014

      Show Abstract
      24586731 24586731
      Synaptic and cognitive improvements by inhibition of 2-AG metabolism are through upregulation of microRNA-188-3p in a mouse model of Alzheimer's disease.
      Zhang, J; Hu, M; Teng, Z; Tang, YP; Chen, C
      The Journal of neuroscience : the official journal of the Society for Neuroscience  34  14919-33  2014

      Show Abstract
      25378159 25378159
      Extracellular ATP hydrolysis inhibits synaptic transmission by increasing ph buffering in the synaptic cleft.
      Vroman, R; Klaassen, LJ; Howlett, MH; Cenedese, V; Klooster, J; Sjoerdsma, T; Kamermans, M
      PLoS biology  12  e1001864  2014

      Show Abstract
      24844296 24844296
      In vitro ischemia triggers a transcriptional response to down-regulate synaptic proteins in hippocampal neurons.
      Fernandes, J; Vieira, M; Carreto, L; Santos, MA; Duarte, CB; Carvalho, AL; Santos, AE
      PloS one  9  e99958  2014

      Show Abstract
      Western Blotting24960035 24960035
      The MK2/3 cascade regulates AMPAR trafficking and cognitive flexibility.
      Eales, KL; Palygin, O; O'Loughlin, T; Rasooli-Nejad, S; Gaestel, M; Müller, J; Collins, DR; Pankratov, Y; Corrêa, SA
      Nature communications  5  4701  2014

      Show Abstract
      Western Blotting25134715 25134715

      Brochure

      Title
      Pathways and Biomarkers of Glutamatergic Synapse Flyer (EMD)

      Data Sheet

      Title
      Anti-Glutamate Receptor 2, extracellular, clone 6C4 - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies