AB1506 Anti-Glutamate Receptor 2 & 3 Antibody

50 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, Mk, Gp, Ht, Gr IH(P), ICC, IHC, IP, WB Rb Affinity Purified Polyclonal Antibody
      Catalogue NumberAB1506
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Glutamate Receptor 2 & 3 Antibody
      Alternate Names
      • AMPA-selective glutamate receptor 2
      • AMPA-selective glutamate receptor 2
      • glutamate receptor 2
      • glutamate receptor, ionotropic
      • AMPA 2
      Background InformationGlutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcategorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition.
      Product Information
      FormatAffinity Purified
      • Human brain tissue and extracts from mouse brain and rat brain tissue.
      PresentationPurified rabbit polyclonal serum lyophilized from buffer containing 0.01M phosphate, 0.09M NaCl, pH 7.2. 1% BSA and 0.1% sodium azide.
      ApplicationThis Anti-Glutamate Receptor 2 & 3 Antibody is validated for use in IH(P), IC, IH, IP, WB for the detection of Glutamate Receptor 2 & 3.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunohistochemistry:
      A previous lot was used on paraformaldehyde or paraformaldehyde/glutaraldehyde fixed tissue with light and electron microscopy. Cryostat and vibratome sections can be used with or without Triton X-100 treatment. Final concentrations used were 1-3 µg/mL.

      When linked to immobilized Protein A, a previous lot of this antibody immunoprecipitated detergent-solubilized receptor from brain or transfected cells.
      Biological Information
      ImmunogenCarboxy terminus peptide of rat GluR2 conjugated to BSA with glutaraldehyde (EGYNVYGIESVKI).
      SpecificityIt recognizes both GluR2 and GluR3 which have nearly identical carboxy terminal sequences. Western blot analysis of rat brain shows two poorly separated bands which comigrate with GluR2 and GluR3 expressed in transfected cells. GluR2 migrates slightly ahead of GluR3. No cross-reaction with GluR1 or GluR4. The antibody appears to recognize GluR 2 & 3 in several other species, but has not been extensively characterized in these cases.
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Monkey
      • Guinea Pig
      • Hamster
      • Gerbil
      Species Reactivity NoteMouse. Expected to cross-react with human, rat, monkey, gerbil, guinea pig, and hamster.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryGlutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, GRIA1-4. The subunit encoded by this gene (GRIA2) is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to render the channel impermeable to Ca(2+). Human and animal studies suggest that pre-mRNA editing is essential for brain function, and defective GRIA2 RNA editing at the Q/R site may be relevant to amyotrophic lateral sclerosis (ALS) etiology. Alternative splicing, resulting in transcript variants encoding different isoforms, (including the flip and flop isoforms that vary in their signal transduction properties), has been noted for this gene. [provided by RefSeq].

      Transcript Variant: This variant (1) encodes isoform 1 (also known as flip isoform). RNA editing (CAG->CGG) changes aa Gln607Arg.

      Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript matching the reference genomic sequence was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.
      Gene Symbol
      • GRIA2
      • GluR-B
      • gluR-B
      • GluR-2
      • GLUR2
      • GLURB
      • HBGR2
      • GluR-K2
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P42262 # Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
      SIZE: 883 amino acids; 98821 Da
      SUBUNIT: Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. May interact with MPP4. Forms a ternary complex with GRIP1 and CSPG4. Interacts with PRKCABP, GRIP1 and GRIP2 (By similarity).
      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
      PTM: Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity).
      SIMILARITY: SwissProt: P42262 ## Belongs to the glutamate-gated ion channel (TC 1.A.10) family.
      MISCELLANEOUS: The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate.
      Molecular Weight108 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on rat brain lysate.

      Western Blot Analysis:
      1:1000 dilution of this antibody detected glutamate receptor 2 & 3 on 10 μg of rat brain lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at +2-8°C or -20°C for up to 6 months after date of receipt.
      Reconstitute to 500 μL with sterile distilled water. Store reconstituted material frozen (-20°C or -80°C) in undiluted aliquots for up to six months. Diluted antibody containing 0.1% sodium azide for Western blot analysis can be stored for several weeks at +2-8°C and reused repeatedly.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Glutamate Receptor 2 3 - 21470652147065
      Anti-Glutamate Receptor 2 3 - 20263862026386
      Anti-Glutamate Receptor 2 3 - 20647142064714
      Anti-Glutamate Receptor 2 3 - LV1495573LV1495573
      Anti-Glutamate Receptor 2 3 - LV1583031LV1583031
      Anti-Glutamate Receptor 2 3 - LV1689937LV1689937
      Anti-Glutamate Receptor 2 3 - LV1785805LV1785805
      Anti-Glutamate Receptor 2 3 - NG1839534NG1839534
      Anti-Glutamate Receptor 2 3 - NG1895144NG1895144
      Anti-Glutamate Receptor 2 & 32475707


      Reference overviewApplicationSpeciesPub Med ID
      Cellular plasticity induced by anti-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor encephalitis antibodies.
      Peng, X; Hughes, EG; Moscato, EH; Parsons, TD; Dalmau, J; Balice-Gordon, RJ
      Annals of neurology  77  381-98  2015

      Show Abstract
      25369168 25369168
      Glutamatergic signaling at the vestibular hair cell calyx synapse.
      Sadeghi, SG; Pyott, SJ; Yu, Z; Glowatzki, E
      The Journal of neuroscience : the official journal of the Society for Neuroscience  34  14536-50  2014

      Show Abstract
      25355208 25355208
      Adult human nasal mesenchymal-like stem cells restore cochlear spiral ganglion neurons after experimental lesion.
      Bas, E; Van De Water, TR; Lumbreras, V; Rajguru, S; Goss, G; Hare, JM; Goldstein, BJ
      Stem cells and development  23  502-14  2014

      Show Abstract
      24172073 24172073
      Distribution of Na,K-ATPase α subunits in rat vestibular sensory epithelia.
      Schuth, O; McLean, WJ; Eatock, RA; Pyott, SJ
      Journal of the Association for Research in Otolaryngology : JARO  15  739-54  2014

      Show Abstract
      ImmunohistochemistryRat25091536 25091536
      GluA1 phosphorylation contributes to postsynaptic amplification of neuropathic pain in the insular cortex.
      Qiu, S; Zhang, M; Liu, Y; Guo, Y; Zhao, H; Song, Q; Zhao, M; Huganir, RL; Luo, J; Xu, H; Zhuo, M
      The Journal of neuroscience : the official journal of the Society for Neuroscience  34  13505-15  2014

      Show Abstract
      25274827 25274827
      Parkin regulates kainate receptors by interacting with the GluK2 subunit.
      Maraschi, A; Ciammola, A; Folci, A; Sassone, F; Ronzitti, G; Cappelletti, G; Silani, V; Sato, S; Hattori, N; Mazzanti, M; Chieregatti, E; Mulle, C; Passafaro, M; Sassone, J
      Nature communications  5  5182  2014

      Show Abstract
      25316086 25316086
      Protease-activated receptor-1 modulates hippocampal memory formation and synaptic plasticity.
      Almonte, AG; Qadri, LH; Sultan, FA; Watson, JA; Mount, DJ; Rumbaugh, G; Sweatt, JD
      Journal of neurochemistry  124  109-22  2013

      Show Abstract
      23113835 23113835
      Locomotor sensitization to ethanol impairs NMDA receptor-dependent synaptic plasticity in the nucleus accumbens and increases ethanol self-administration.
      Abrahao, KP; Ariwodola, OJ; Butler, TR; Rau, AR; Skelly, MJ; Carter, E; Alexander, NP; McCool, BA; Souza-Formigoni, ML; Weiner, JL
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  4834-42  2013

      Show Abstract
      Western Blotting23486954 23486954
      Two cell circuits of oriented adult hippocampal neurons on self-assembled monolayers for use in the study of neuronal communication in a defined system.
      Edwards, D; Stancescu, M; Molnar, P; Hickman, JJ
      ACS chemical neuroscience  4  1174-82  2013

      Show Abstract
      23611164 23611164
      Suppressing aberrant GluN3A expression rescues synaptic and behavioral impairments in Huntington's disease models.
      Marco, S; Giralt, A; Petrovic, MM; Pouladi, MA; Martínez-Turrillas, R; Martínez-Hernández, J; Kaltenbach, LS; Torres-Peraza, J; Graham, RK; Watanabe, M; Luján, R; Nakanishi, N; Lipton, SA; Lo, DC; Hayden, MR; Alberch, J; Wesseling, JF; Pérez-Otaño, I
      Nature medicine  19  1030-8  2013

      Show Abstract
      23852340 23852340


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