AB5018 Anti-Glutamate (No Glutaraldehyde) Antibody

AB5018
2000 assays  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      A IHC Rb Purified Polyclonal Antibody
      Description
      Catalogue NumberAB5018
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Glutamate (No Glutaraldehyde) Antibody
      References
      Product Information
      FormatPurified
      PresentationPurified immunoglobulin. Liquid in stabilizing buffer (Phosphate buffer with 1% sterile goat serum) containing 0.05% thimerosal.
      Applications
      ApplicationThis Anti-Glutamate (No Glutaraldehyde) Antibody is validated for use in IH for the detection of Glutamate (No Glutaraldehyde).
      Key Applications
      • Immunohistochemistry
      Application NotesImmunohistochemistry using silver-intensified immunogold or fluorescence (see recommended protocol). Samples should be fixed with 0.1% - 2.5% glutaraldehyde for optimum detection.

      *This antibody has also been used and found to work with a zero-low glutaraldehyde / high paraformaldehyde fixation (4% paraformaldehyde in 0.1M phosphate buffer / 3% sucrose fixative ). The minimum glutaraldehyde concentration for AB5018 is 0.00%. See protocol that follows. Performance is good with frozen sections, Vibratome sections and tissue culture formats, when penetrating reagents such as 0.3% Triton X-100 are used.

      Optimal working dilutions must be determined by the end user.

      DILUTION: Prepare enough of the AB5018 for your days use by diluting 100X with 1% GSPBT.
      Biological Information
      ImmunogenGlutamate-glutaraldehyde
      HostRabbit
      SpecificityGlutamate (L-Glutamate). The antibody has been calibrated against a spectrum of antigens to assure hapten selectivity and proper affinity. No measurable cross-reactivity (<1:1000) against glutamate in peptides or proteins. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) against L-alanine, gamma-aminobutyrate, 1-amino-4-guanidobutane (AGB), D/L-arganine, D/L-aspartate, L-citrulline, L-cysteine, D/L-glutamine, glutathione, glycine, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine. Modest cross-reactivity (1:20) against D-glutamate. Significant cross-reactivity (1:8) against free NAAG in competition assays (NAAG is not retained in glutaraldehyde-fixed tissue).
      Species Reactivity
      • All
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain stock at 2-8°C in undiluted aliquots for up to 6 months. This stock is extremely stable under normal use and routine storage at 2-8°C. Do not freeze.
      Packaging Information
      Material Size2000 assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-GLUTAMATE -27366812736681
      RABBIT ANTI-GLUTAMATE -27888942788894
      RABBIT ANTI-GLUTAMATE -27895802789580
      RABBIT ANTI-GLUTAMATE -28317932831793
      RABBIT ANTI-GLUTAMATE POLYCLONAL ANTIBODY2952619
      RABBIT ANTI-GLUTAMATE POLYCLONAL ANTIBODY2891415
      RABBIT ANTI-GLUTAMATE POLYCLONAL ANTIBODY3091949
      RABBIT ANTI-GLUTAMATE POLYCLONAL ANTIBODY - 20547872054787
      RABBIT ANTI-GLUTAMATE POLYCLONAL ANTIBODY - 23616202361620
      RABBIT ANTI-GLUTAMATE POLYCLONAL ANTIBODY - 25062972506297

      References

      Reference overviewApplicationSpeciesPub Med ID
      Retinal amino acid neurochemistry of the southern hemisphere lamprey, Geotria australis.
      Nivison-Smith, L; Collin, SP; Zhu, Y; Ready, S; Acosta, ML; Hunt, DM; Potter, IC; Kalloniatis, M
      PloS one  8  e58406  2013

      Show Abstract
      23516473 23516473
      Glutamate, excitatory amino acid transporters, Xc- antiporter, glutamine synthetase, and gamma-glutamyltranspeptidase in human corneal epithelium.
      Marlyn P Langford,Patrick Redmond,Raul Chanis,Raghunath P Misra,Thomas B Redens
      Current eye research  35  2010

      Show Abstract
      20373878 20373878
      Virus-induced changes in the subcellular distribution of glutathione precursors in Cucurbita pepo (L.).
      Zechmann, B, et al.
      Plant Biol (Stuttg), 9: 427-34 (2007)  2007

      Show Abstract
      17143806 17143806
      Depletion of taurine and glutamate from damaged photoreceptors in the retinas of dogs with primary glaucoma.
      James E Madl, Tonya R McIlnay, Cynthia C Powell, Juliet R Gionfriddo
      American journal of veterinary research  66  791-9  2005

      Show Abstract
      15934606 15934606
      Neurochemical phenotypes of MRF neurons influencing diaphragm and rectus abdominis activity.
      Billig, I, et al.
      J. Appl. Physiol., 94: 391-8 (2003)  2003

      Show Abstract
      Immunohistochemistry (Tissue)Ferret12391091 12391091
      Psychostimulants differentially regulate serotonin transporter expression in thalamocortical neurons.
      Whitworth, Terri L, et al.
      J. Neurosci., 22: RC192 (2002)  2002

      Show Abstract
      Immunohistochemistry (Tissue)Rat11756522 11756522

      Brochure

      Title
      Pathways and Biomarkers of Glutamatergic Synapse Flyer (EMD)

      Data Sheet

      Title
      RABBIT ANTI-GLUTAMATE POLYCLONAL ANTIBODY