IF03L Anti-Glial Fibrillary Acidic Protein (GFAP) Mouse mAb (G-A-5)

IF03L
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      Ch, H, Porcine, R M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      IF03L-100UG
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      Stocked 
      Discontinued
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      Available
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          Will advise
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          Contact Customer Service

          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes GFAP in astrocytes, astrocytomas, glial cells, gliomas, and other glial cell-derived tumors.
          Catalogue NumberIF03L
          Brand Family Calbiochem®
          SynonymsAnti-GFAP
          References
          ReferencesDroese, M., et al. 1984. Acta. Cytol. 28, 368.
          Osborn, M., et al. 1984. In: Cancer Cells 1, The Transformed Phenotype. Cold Spring Harbor Lab., 191.
          Geisler, N. and Weber, K. 1983. EMBO J. 11, 2059.
          Osborn, M. and Weber, K. 1983. Lab. Invest. 48, 372.
          Altmannsberger, M., et al. 1982. Lab. Invest. 46, 520.
          Lazarides, E. 1982. Ann. Rev. Biochem. 51, 219.
          Osborn, M. and Weber, K. 1982. Cell 31, 303.
          Product Information
          FormLyophilized
          FormulationLyophilized from 20 mM NH₄HCO₃, 100 µg BSA.
          Negative controlEpithelial cells
          Positive controlGlial cells (such as U343/333MG)
          PreservativeNone
          Applications
          Application ReferencesImmunoblotting, Paraffin Sections Sennlaub, F., et al. 2003. Circulation 108, 198. Original Clone Debus, E., et al. 1983. Differentiation 25, 193. Immunofluorescence Debus, E., et al. 1983. Differentiation 25, 193.
          Key Applications Immunoprecipitation
          Paraffin Sections
          Frozen Sections
          Immunoblotting (Western Blotting)
          Immunofluorescence
          Application NotesImmunoblotting (see application references)
          Paraffin Sections (see application references)
          Frozen Sections (see comments)
          Immunofluorescence (see application references)
          Immunoprecipitation (see comments)
          Application CommentsStains intermediate filaments in astrocytes, glial cells, gliomas, and other glial cell-derived tumors. This antibody has also been reported to work for frozen sections and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          ImmunogenGFAP
          ImmunogenPorcine
          CloneG-A-5
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Chicken
          • Human
          • Porcine
          • Rat
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsResuspend the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Freezing of aliquots is best for long-term storage of reconstituted product; avoid repetitive freezing and thawing.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-Glial Fibrillary Acidic Protein (GFAP) Mouse mAb (G-A-5) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-Glial Fibrillary Acidic Protein (GFAP) Mouse mAb (G-A-5) Certificates of Analysis

          TitleLot Number
          IF03L

          References

          Reference overview
          Droese, M., et al. 1984. Acta. Cytol. 28, 368.
          Osborn, M., et al. 1984. In: Cancer Cells 1, The Transformed Phenotype. Cold Spring Harbor Lab., 191.
          Geisler, N. and Weber, K. 1983. EMBO J. 11, 2059.
          Osborn, M. and Weber, K. 1983. Lab. Invest. 48, 372.
          Altmannsberger, M., et al. 1982. Lab. Invest. 46, 520.
          Lazarides, E. 1982. Ann. Rev. Biochem. 51, 219.
          Osborn, M. and Weber, K. 1982. Cell 31, 303.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision27-August-2007 RFH
          SynonymsAnti-GFAP
          ApplicationImmunoblotting (see application references)
          Paraffin Sections (see application references)
          Frozen Sections (see comments)
          Immunofluorescence (see application references)
          Immunoprecipitation (see comments)
          DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with PAI mouse myeloma cells (see application references). Recognizes the glial fibrillary acidic protein (GFAP).
          BackgroundIntermediate (10 nm) filaments are present in, and provide information concerning the origin of, most vertebrate cells. At present, five major cell types can be distinguished by their intermediate filament components. These include epithelial cells (cytokeratins), neurons (neurofilaments), glial cells (glial fibrillary acidic protein or GFA or GFAP), skeletal, visceral, and certain vascular smooth muscle cells (desmin) and various nonepithelial cells, including cells of mesenchymal origin (vimentin). GFAP and vimentin are coexpressed in at least some astrocytes. Anti-GFAP (Ab-1) identifies the GFAP subclass of intermediate filaments in human tissues.
          HostMouse
          Immunogen speciesPorcine
          ImmunogenGFAP
          CloneG-A-5
          IsotypeIgG₁
          Specieschicken, human, porcine, rat
          Positive controlGlial cells (such as U343/333MG)
          Negative controlEpithelial cells
          FormLyophilized
          FormulationLyophilized from 20 mM NH₄HCO₃, 100 µg BSA.
          PreservativeNone
          CommentsStains intermediate filaments in astrocytes, glial cells, gliomas, and other glial cell-derived tumors. This antibody has also been reported to work for frozen sections and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsResuspend the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Freezing of aliquots is best for long-term storage of reconstituted product; avoid repetitive freezing and thawing.
          Toxicity Standard Handling
          ReferencesDroese, M., et al. 1984. Acta. Cytol. 28, 368.
          Osborn, M., et al. 1984. In: Cancer Cells 1, The Transformed Phenotype. Cold Spring Harbor Lab., 191.
          Geisler, N. and Weber, K. 1983. EMBO J. 11, 2059.
          Osborn, M. and Weber, K. 1983. Lab. Invest. 48, 372.
          Altmannsberger, M., et al. 1982. Lab. Invest. 46, 520.
          Lazarides, E. 1982. Ann. Rev. Biochem. 51, 219.
          Osborn, M. and Weber, K. 1982. Cell 31, 303.
          Application referencesImmunoblotting, Paraffin Sections Sennlaub, F., et al. 2003. Circulation 108, 198. Original Clone Debus, E., et al. 1983. Differentiation 25, 193. Immunofluorescence Debus, E., et al. 1983. Differentiation 25, 193.