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NE1015 | Anti-Glial Fibrillary Acidic Protein Cocktail Mouse mAb (SMI-22)

NE1015
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      B, Ca, Ch, Gp, H, M, Porcine, R, Sh M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      NE1015-100UL
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          Glass bottle 100 ul
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          Description
          OverviewRecognizes ~50 kDa glial fibrillary acidic protein (GFAP) in human and bovine cytoskeletal preparations.
          Catalogue NumberNE1015
          Brand Family Calbiochem®
          SynonymsAnti-GFAP Cocktail
          Application Data
          Detection of rat glial fibrillary acidic protein by staining frozen sections. Sample: Rat brain. Primary antibody: Anti-GFAP Cocktail Mouse mAb (SMI-22) (Cat. No. NE1015) (1:1000). Detection: fluorescence (red) with Hoechst 33342 counterstain.

          Primary mixed glial cultures were stained with anti-mouse GFAP (EMD Millipore, Cat. No. MAB360) and a DyLight™488 conjugated goat anti-mouse secondary Antibody. The nucleus was counterstained with DAPI. The image was captured using Olympus BX51 with an exposure time of 982 millisec for green channel and 126 millisec for DAPI channel. Magnification is 10X without any correction on Gain/Offset/Bad pixel. Courtesy of Jose Shinsmon, Immunology, Dept of Pathology, Faculty of Medicine and Health Sciences, UPM,Serdang 43400.
          References
          ReferencesVick, W.W., et al. 1987. Acta. Cytol. 31, 816.
          McLendon R.E., et al. 1986. J. Neuropathol. Exp. Neurol. 45, 692.
          Pegram, C.N., et al. 1985. Neurochem. Pathol. 3, 119.
          Product Information
          FormLiquid
          FormulationUndiluted ascites.
          Positive controlAstrocytes or cytoskeletal preparations
          Preservative≤ 0.1% sodium azide
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Frozen Sections
          Immunoblotting (Western Blotting)
          Immunocytochemistry
          Paraffin Sections
          Application NotesELISA (1:1000)
          Frozen Sections (1:1000, see comments)
          Immunoblotting (1:1000)
          Immunocytochemistry (1:1000, see comments)
          Paraffin Sections (1:1000, trypsin or heat pre-treatment required)
          Application CommentsThis cocktail is derived from the Bigner-Eng clones MAb1B4, MAb2E1, and MAb4A11 and provides a means for more comprehensive detection of astrocytomas than each clone alone. Each component is specific for GFAP and stains astrocytes and astrocytic processes as well as Bergman glia. Recognizes both anaplastic and reactive astrocytes by immunocytochemical staining. Does not recognize metastatic tumors and brain tumors of non-astrocytic origin, including medulloblastomas, meningiomas, choroid plexus papillomas, and schwannomas. For staining paraffin sections it is recommended that de-paraffinized sections be treated with 0.1% trypsin in 50 mM Tris-HCl, pH 7.6 for 20-30 min at 37°C or boiled in Tris-buffered saline, pH 9.0 for 15 min to expose the epitope. For immunocytochemistry or staining frozen sections, post-fixation in cold methanol or methanol/hydrogen peroxide for 10 min is required for access to the astrocytes in the sample. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenpurified bovine GFAP protein
          ImmunogenBovine
          CloneSMI-22
          HostMouse
          IsotypeIgG2b
          Species Reactivity
          • Bovine
          • Canine
          • Chicken
          • Guinea Pig
          • Human
          • Mouse
          • Porcine
          • Rat
          • Sheep
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsUpon initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          NE1015

          References

          Reference overview
          Vick, W.W., et al. 1987. Acta. Cytol. 31, 816.
          McLendon R.E., et al. 1986. J. Neuropathol. Exp. Neurol. 45, 692.
          Pegram, C.N., et al. 1985. Neurochem. Pathol. 3, 119.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision01-October-2007 RFH
          SynonymsAnti-GFAP Cocktail
          ApplicationELISA (1:1000)
          Frozen Sections (1:1000, see comments)
          Immunoblotting (1:1000)
          Immunocytochemistry (1:1000, see comments)
          Paraffin Sections (1:1000, trypsin or heat pre-treatment required)
          Application Data
          Detection of rat glial fibrillary acidic protein by staining frozen sections. Sample: Rat brain. Primary antibody: Anti-GFAP Cocktail Mouse mAb (SMI-22) (Cat. No. NE1015) (1:1000). Detection: fluorescence (red) with Hoechst 33342 counterstain.

          Primary mixed glial cultures were stained with anti-mouse GFAP (EMD Millipore, Cat. No. MAB360) and a DyLight™488 conjugated goat anti-mouse secondary Antibody. The nucleus was counterstained with DAPI. The image was captured using Olympus BX51 with an exposure time of 982 millisec for green channel and 126 millisec for DAPI channel. Magnification is 10X without any correction on Gain/Offset/Bad pixel. Courtesy of Jose Shinsmon, Immunology, Dept of Pathology, Faculty of Medicine and Health Sciences, UPM,Serdang 43400.
          DescriptionMouse monoclonal antibody cocktail that contains a mixture of 3 antibodies supplied as undiluted ascites. Recognizes the ~50 kDa glial fibrillary acidic protein.
          BackgroundGlial fibrillary acidic protein (GFAP) is an intermediate filament protein found only in glial cells or cells of glial origin. It can be detected in astrocytes and certain other astroglia in the CNS, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. GFAP is upregulated and expressed at high levels in astrocytes in many damage and disease states. It is also often highly expressed in neural stem cells and many types of brain tumors.
          HostMouse
          Immunogen speciesBovine
          Immunogenpurified bovine GFAP protein
          CloneSMI-22
          IsotypeIgG2b
          Speciesbovine, canine, chicken, guinea pig, human, mouse, porcine, rat, sheep
          Positive controlAstrocytes or cytoskeletal preparations
          FormLiquid
          FormulationUndiluted ascites.
          Preservative≤ 0.1% sodium azide
          CommentsThis cocktail is derived from the Bigner-Eng clones MAb1B4, MAb2E1, and MAb4A11 and provides a means for more comprehensive detection of astrocytomas than each clone alone. Each component is specific for GFAP and stains astrocytes and astrocytic processes as well as Bergman glia. Recognizes both anaplastic and reactive astrocytes by immunocytochemical staining. Does not recognize metastatic tumors and brain tumors of non-astrocytic origin, including medulloblastomas, meningiomas, choroid plexus papillomas, and schwannomas. For staining paraffin sections it is recommended that de-paraffinized sections be treated with 0.1% trypsin in 50 mM Tris-HCl, pH 7.6 for 20-30 min at 37°C or boiled in Tris-buffered saline, pH 9.0 for 15 min to expose the epitope. For immunocytochemistry or staining frozen sections, post-fixation in cold methanol or methanol/hydrogen peroxide for 10 min is required for access to the astrocytes in the sample. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsUpon initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesVick, W.W., et al. 1987. Acta. Cytol. 31, 816.
          McLendon R.E., et al. 1986. J. Neuropathol. Exp. Neurol. 45, 692.
          Pegram, C.N., et al. 1985. Neurochem. Pathol. 3, 119.