MAB360 Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5

MAB360
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, Ch, H, M, Po, R, Rb ICC, IHC, IH(P), WB M Ascites Monoclonal Antibody
      Description
      Catalogue NumberMAB360
      Replaces04-1031; 04-1062
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Glial Fibrillary Acidic Protein Antibody, clone GA5
      Alternate Names
      • GFAP
      Background InformationGlial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.
      References
      Product Information
      FormatAscites
      Control
      • Positive Control: Cultured astrocytes, brain tissue (white matter), spinal chord, retina
      PresentationUnpurified mouse monoclonal IgG1 liquid in buffer containing 15 mM sodium azide.
      Applications
      ApplicationAnti-Glial Fibrillary Acidic Protein Antibody, clone GA5 detects level of Glial Fibrillary Acidic Protein & has been published & validated for use in IC, IH, IH(P) & WB with more than 65 product citations.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunocytochemistry:
      A previous lot was used on cultured mammalian cells.

      Immunohistochemistry(paraffin):
      1:400-1:800 dilution from a previous lot was used on alcohol-fixed paraffin embedded sections of rat brain (cerebrum or cerebellum) and human brain.

      Immunoblotting: A 1:1000 dilution of a previous lot was used.

      Optimal working dilutions must be determined by the end user
      Biological Information
      ImmunogenPurified GFAP from porcine spinal cord.
      CloneGA5
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityGlial fibrillar acidic protein. On western blots of extracts from a human glioma cell line (U33CG/343MG), MAB360 recognizes a band at approximately 51 kDa corresponding to GFAP (Debus, 1983). By immunohistochemistry it recognizes astrocytes and Bergmann glia cells, glioma and glial cell derived tumors. Shows no cross-reactivity with vimentin.
      IsotypeIgG1
      Species Reactivity
      • Bovine
      • Chicken
      • Human
      • Mouse
      • Pig
      • Rat
      • Rabbit
      Species Reactivity NoteReacts with Bovine, Chicken, Human, Mouse, Porcine (Pig), Rabbit and Rat. Reactivity with other species has not been determined.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined.
      Gene Symbol
      • GFAP
      • FLJ45472
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P14136 # GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
      SIZE: 432 amino acids; 49880 Da
      SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1.
      SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
      DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander disease [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
      SIMILARITY: SwissProt: P14136 ## Belongs to the intermediate filament family.
      Molecular Weight~ 51 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Mouse brain lysates.

      Western Blot Analysis:
      1:1000 dilution of this lot detected GFAP on 10 μg of Mouse brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Glial Fibrillary Acidic Protein, -25493612549361
      Anti-Glial Fibrillary Acidic Protein, -27985782798578
      Anti-Glial Fibrillary Acidic Protein, clone GA53074992
      Anti-Glial Fibrillary Acidic Protein, clone GA5 (mouse monoclonal) Monoclonal AntibodyQ2909944
      Anti-Glial Fibrillary Acidic Protein, clone GA5 - 23280182328018
      Anti-Glial Fibrillary Acidic Protein, clone GA5 - 23905232390523
      Anti-Glial Fibrillary Acidic Protein, clone GA5 - 24467692446769
      Anti-Glial Fibrillary Acidic Protein, clone GA5 - 19405271940527
      Anti-Glial Fibrillary Acidic Protein, clone GA5 - 19870501987050
      Anti-Glial Fibrillary Acidic Protein, clone GA5 - 20411042041104

      References

      Reference overviewApplicationSpeciesPub Med ID
      Early pro-inflammatory cytokine elevations in the DBA/2J mouse model of glaucoma.
      Wilson, GN; Inman, DM; Dengler Crish, CM; Denger-Crish, CM; Smith, MA; Crish, SD
      Journal of neuroinflammation  12  176  2015

      Show Abstract
      26376776 26376776
      Dopamine D1 receptor activation regulates the expression of the estrogen synthesis gene aromatase B in radial glial cells.
      Xing, L; McDonald, H; Da Fonte, DF; Gutierrez-Villagomez, JM; Trudeau, VL
      Frontiers in neuroscience  9  310  2015

      Show Abstract
      26388722 26388722
      Effects of dextromethorphan and oxycodone on treatment of neuropathic pain in mice.
      Yang, PP; Yeh, GC; Huang, EY; Law, PY; Loh, HH; Tao, PL
      Journal of biomedical science  22  81  2015

      Show Abstract
      26391752 26391752
      Buyang Huanwu decoction increases the expression of glutamate transporter-1 and glutamate synthetase in association with PACAP-38 following focal ischemia.
      Ding, W; Yu, P; Liu, W; Zhou, L; Guan, LI; Lin, R
      Biomedical reports  3  651-656  2015

      Show Abstract
      26405540 26405540
      NFκB signaling drives pro-granulocytic astroglial responses to neuromyelitis optica patient IgG.
      Walker-Caulfield, ME; Guo, Y; Johnson, RK; McCarthy, CB; Fitz-Gibbon, PD; Lucchinetti, CF; Howe, CL
      Journal of neuroinflammation  12  185  2015

      Show Abstract
      26423139 26423139
      Protein carbonylation after traumatic brain injury: cell specificity, regional susceptibility, and gender differences.
      Lazarus, RC; Buonora, JE; Jacobowitz, DM; Mueller, GP
      Free radical biology & medicine  78  89-100  2015

      Show Abstract
      25462645 25462645
      Spinal autophagy is differently modulated in distinct mouse models of neuropathic pain.
      Berliocchi, L; Maiarù, M; Varano, GP; Russo, R; Corasaniti, MT; Bagetta, G; Tassorelli, C
      Molecular pain  11  3  2015

      Show Abstract
      25645145 25645145
      Evidence for a novel functional role of astrocytes in the acute homeostatic response to high-fat diet intake in mice.
      Buckman, LB; Thompson, MM; Lippert, RN; Blackwell, TS; Yull, FE; Ellacott, KL
      Molecular metabolism  4  58-63  2015

      Show Abstract
      25685690 25685690
      Cuprizone-induced demyelination and demyelination-associated inflammation result in different proton magnetic resonance metabolite spectra.
      Praet, J; Orije, J; Kara, F; Guglielmetti, C; Santermans, E; Daans, J; Hens, N; Verhoye, M; Berneman, Z; Ponsaerts, P; Van der Linden, A
      NMR in biomedicine  28  505-13  2015

      Show Abstract
      25802215 25802215
      The clinical heterogeneity of coenzyme Q10 deficiency results from genotypic differences in the Coq9 gene.
      Luna-Sánchez, M; Díaz-Casado, E; Barca, E; Tejada, MÁ; Montilla-García, Á; Cobos, EJ; Escames, G; Acuña-Castroviejo, D; Quinzii, CM; López, LC
      EMBO molecular medicine  7  670-87  2015

      Show Abstract
      25802402 25802402

      Data Sheet

      Title
      Anti-Glial Fibrillary Acidic Protein, clone GA5 - Data Sheet

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies