371729 Anti-Giα-3-Subunit, C-Terminal (345-354) Rabbit pAb

371729
  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      Ma Rb Polyclonal Antibody
      Description
      OverviewRecognizes Giα-3 subunit. Does not cross-react with Gsα and Goα, but partially cross-reacts with Giα1-, G1α2-subunits.
      Catalogue Number371729
      Brand Family Calbiochem®
      References
      ReferencesKumar, R., et al. 1994. J. Mol. Cell. Cardiol. 26, 1537.
      Raymond, J.R., et al. 1993. Biochemistry 32, 11064.
      Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol. 195, 215.
      Jones, D.T., and Reed, R.R. 1987. J. Biol. Chem. 262, 14,241.
      Product Information
      FormLiquid
      FormulationIn 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.
      PreservativeNone
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1:1000)
      Immunoprecipitation (see comments)
      Application CommentsThe specificity was confirmed with lysates from separate cultures of bacteria transfected with the genes for Gsα, Giα-1, GIα-2, Giα-3, and Goα. The original titer of the serum was 1:10,000. Suitable for immunoblotting and immunoprecipitation. Does not cross-react with Gsα and Goα. Antibody partially cross-reacts with Giα-1 and GIα-2. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic peptide (CKNNLKECGLY) corresponding to amino acids at the C-terminus of mammalian Giα-3, conjugated to KLH
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Mammals
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Giα-3-Subunit, C-Terminal (345-354) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Giα-3-Subunit, C-Terminal (345-354) Rabbit pAb Certificates of Analysis

      TitleLot Number
      371729

      References

      Reference overview
      Kumar, R., et al. 1994. J. Mol. Cell. Cardiol. 26, 1537.
      Raymond, J.R., et al. 1993. Biochemistry 32, 11064.
      Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol. 195, 215.
      Jones, D.T., and Reed, R.R. 1987. J. Biol. Chem. 262, 14,241.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-August-2007 RFH
      ApplicationImmunoblotting (1:1000)
      Immunoprecipitation (see comments)
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the Giα-3 subunit protein.
      HostRabbit
      Immunogena synthetic peptide (CKNNLKECGLY) corresponding to amino acids at the C-terminus of mammalian Giα-3, conjugated to KLH
      IsotypeIgG
      Speciesmammalian
      FormLiquid
      FormulationIn 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.
      PreservativeNone
      CommentsThe specificity was confirmed with lysates from separate cultures of bacteria transfected with the genes for Gsα, Giα-1, GIα-2, Giα-3, and Goα. The original titer of the serum was 1:10,000. Suitable for immunoblotting and immunoprecipitation. Does not cross-react with Gsα and Goα. Antibody partially cross-reacts with Giα-1 and GIα-2. Variables associated with assay conditions will dictate the proper working dilution.
      Storage ≤ -70°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesKumar, R., et al. 1994. J. Mol. Cell. Cardiol. 26, 1537.
      Raymond, J.R., et al. 1993. Biochemistry 32, 11064.
      Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol. 195, 215.
      Jones, D.T., and Reed, R.R. 1987. J. Biol. Chem. 262, 14,241.