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ABE1934 | Anti-Gcn5 Antibody

ABE1934
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M IP, WB, ChIP-seq, ChIP Rb Purified Polyclonal Antibody
      Description
      Catalogue NumberABE1934
      DescriptionAnti-Gcn5 Antibody
      Alternate Names
      • Histone acetyltransferase KAT2A
      • General control of amino acid synthesis protein 5-like 2
      • hGCN5
      • Histone acetyltransferase GCN5
      • HsGCN5
      • Lysine acetyltransferase 2A
      • STAF97
      Background InformationHistone acetyltransferase KAT2A (EC 2.3.1.48; UniProt Q92830; also known as General control of amino acid synthesis protein 5-like 2, hGCN5, Histone acetyltransferase GCN5, HsGCN5, Lysine acetyltransferase 2A, STAF97) is encoded by the KAT2A (also known as GCN5, GCN5L2, HGCN5) gene (Gene ID 2648) in human. Histone acetyltransferases (HATs) are transcription co-activators recruited by transcriptional activators to target gene promoters, where they enhance the transcription activity by acetylating lysine residues within the N-terminal regions of histones. KAT2A/KAT2B, MYST (MOZ, Ybf2 (Sas3), Sas2, and Tip60), TAFII250, CREBBP/EP300, and SRC represent the five HAT families. In addtion to functioning as transcription co-activators, KAT2A (GCN5) and KAT2B (PCAF) are also reported to repress IFN‐β production and innate antiviral immunity by inhibiting the innate immune signaling kinase TBK1 in the cytoplasm in a HAT‐independent and non‐transcriptional manner.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Gcn5 Antibody is an antibody against Gcn5 for use in Immunoprecipitation, Western Blotting, ChIP-seq, Chromatin Immunoprecipitation (ChIP).
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • ChIP-seq
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesWestern Blotting Analysis: 1.2 µg/mL from a representative lot detected Gcn5 immunoprecipited from HeLa nuclear extract.
      Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot immunoprecipitated KAT2A/GCN5-associated DNA fragments by ChIP using chromatin preparations from murine CH12 and human GM12878 lymphoblasts (Cheng, Y., et al. (2014). Nature. 515(7527):371-375).
      ChIP-seq Analysis: A representative lot was employed to generate Gcn5 genome-wide binding profiles by ChIP-seq analysis using chromatin preparations from murine CH12 and human GM12878 lymphoblasts (Cheng, Y., et al. (2014). Nature. 515(7527):371-375).
      Biological Information
      ImmunogenOvalbumin-conjugated linear peptide corresponding to the N-terminal sequence of human Gcn5.
      EpitopeN-terminus.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody was raised against human KAT2A isoform GCN5-L N-terminal sequence that is 95% conserved in murine and rat Kat2a/Gcn5, but missing in human spliced isoform GCN5-S (UniProt Q92830-2).
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • KAT2A
      • GCN5
      • GCN5L2
      • HGCN5
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight~100 kDa observed. 93.93 kDa (human GCN5-L; UniProt Q92830-1) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunoprecipitation in HeLa nuclear extract.

      Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipited Gcn5 from 500 µg of HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications