A part of MilliporeSigma

07-1413 | Anti-GSK-3b Antibody, a.a. 335-349

50 µg  
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      Replacement Information
      Replacement Information07-1413 is a recommended replacement for AB8687

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, H, M, B WB, IP, IH(P) Rb Affinity Purified Polyclonal Antibody
      Catalogue Number07-1413
      DescriptionAnti-GSK-3b Antibody, a.a. 335-349
      Background InformationGSK3 (Glycogen Synthase Kinase 3) differs from most serine/threonine kinases in that it is active in the absence of the action of signaling pathways. Two isoforms exist, GSK3α and GSK3β. The function of GSK3 is to phosphorylate Glycogen Synthase and thereby inactivate it. Insulin action stimulates the PI3 Kinase pathway, resulting in Akt activation, which phosphorylates and inactivates GSK3. Glycogen Synthase is then rapidly dephosphorylated and activated. Other GSK3 substrates include Jun (on inhibitory sites), and eIF2B. Phosphorylation of Tau by GSK3 may relate to development of Alzheimer's disease. Phosphorylation state-specific antibodies directed against the Akt site (Ser21) on GSK3 are suitable for surrogate assays of the activation state of the pathway.
      Product Information
      FormatAffinity Purified
      • C2C12 Cell Lysate, Hela cells, rat or mouse brain
      PresentationPurified polyclonal IgG antibody is supplied in PBS, pH 7.2, 50% glycerol and 0.09% sodium azide.
      ApplicationThis AntiI-GSK-3b Antibody, a.a. 335-349 is validated for use in WB, IP, IH(P) for the detection of AntiI-GSK-3b.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunohistochemistry (Paraffin)
      Application NotesImmunoprecipitation: 12.5 µg/mL dilution of a previous lot was ued in immunoprecipitation on rat brain tissue; immunoprecipitations with tissue extracts other than brain may require higher antibody concentrations.
      Optimal working dilutions must be determined by the end user.

      Representative testing from a previous lot.
      Optimal Staining of GSK3β Polyclonal Antibody: HeLa Cells
      Biological Information
      ImmunogenRat GSK3 beta synthetic peptide (aa. 335-349), conjugated to KLH. This sequence is 100% identical to human GSK3 beta.
      Epitopea.a. 335-349
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody detects a strong 47 kDa protein, corresponding to the expected molecular mass of GSK3 beta.
      Species Reactivity
      • Rat
      • Human
      • Mouse
      • Bovine
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryGlycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and nactivating glycogen synthase. Two isoforms, alpha (GSK3A; MIM 606784) and beta, show a high degree of amino acid homology (Stambolic and Woodgett, 1994 [PubMed 7980435]). GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation (Plyte et al., 1992 [PubMed1333807]).[supplied by OMIM].
      Gene Symbol
      • GSK3B
      • EC
      Purification MethodProtein A affinity purified
      UniProt Number
      UniProt SummaryFUNCTION: Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin.
      CATALYTIC ACTIVITY: ATP + [tau protein] = ADP + [tau protein] phosphate.
      ENZYME REGULATION: Inhibited when phosphorylated by AKT1.
      SUBUNIT: Monomer (By similarity). Interacts with CABYR, MUC1, NIN and PRUNE.
      ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.
      Molecular WeightThis antibody detects a strong 47 kDa protein.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on C2C12 lysates.

      Western Blot Analysis: 1:500 dilution of this lot detected GSK3 BETA on 10 μg of C2C12 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable at –20 ºC for up to one year from date of receipt. Product may be stored at for 2-4 weeks at 2º-8ºC.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-GSK3#946; - 2128250 2128250
      Anti-GSK3#946; - 2148297 2148297
      Anti-GSK3#946; - NG1895550 NG1895550
      Anti-GSK3b - 2040404 2040404
      Anti-GSK3b - NG1605297 NG1605297
      Anti-GSK3b - NG1630668 NG1630668
      Anti-GSK3b - NG1725537 NG1725537
      Anti-GSK3β - 2383594 2383594
      Anti-GSK3β - 2462736 2462736
      Anti-GSK3β -2488709 2488709
      Anti-GSK3β -2519205 2519205
      Anti-GSK3β -2653121 2653121
      Anti-GSK3β -2689038 2689038
      Anti-GSK3β -2712292 2712292
      Anti-GSK3β -2736705 2736705
      Anti-GSK3 -2822720 2822720


      Reference overviewPub Med ID
      Impact of laminitis on the canonical Wnt signaling pathway in basal epithelial cells of the equine digital laminae.
      Wang, L; Pawlak, EA; Johnson, PJ; Belknap, JK; Eades, S; Stack, S; Cousin, H; Black, SJ
      PloS one 8 e56025 2013

      Show Abstract
      23405249 23405249
      The neuron-specific isoform of glycogen synthase kinase-3beta is required for axon growth.
      Castaño, Zafira, et al.
      J. Neurochem., 113: 117-30 (2010) 2010

      Show Abstract
      20067585 20067585

      Newsletters / Publications

      Research Focus - Volume 2, 2013

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      Life Science Research > Antibodies and Assays > Primary Antibodies