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MABS474 | Anti-GRP78 Antibody, clone N88

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M WB, ACT, FC M Purified Monoclonal Antibody
      Catalogue NumberMABS474
      DescriptionAnti-GRP78 Antibody, clone N88
      Alternate Names
      • 78 kDa glucose-regulated protein
      • GRP-78
      • Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
      • Heat shock 70 kDa protein 5
      • Immunoglobulin heavy chain-binding protein
      • BiP
      Background InformationThe gene HSPA5 encodes a protein named GRP-78 (78 kDa glucose-regulated protein) also known as BiP (immunoglobulin heavy chain-binding protein) or heat shock 70 kDa protein 5. While originally discovered as one of set of genes that were induced upon cellular starvation and stress, HSPA5 encoded GRP-78 is now known to be an important member of the heat-shock family of proteins. The HSPA5 encoded GRP-78 protein appears to play a role in helping in the assembly of multimeric protein complexes in the endoplasmic reticulum (ER). Cellular localization is in the endoplasmic reticulum lumen and appears as cytoplasmic-like staining. The HSPA5 encoded protein is an essential component of the translocation processes within the endoplasmic reticulum and also functions in the retrograde transport across the ER membrane of incorrectly folded proteins destined to be degraded by the ubiquitin proteasome system. The HSPA5 encoded protein, while widely expressed in all cells types under normal conditions, is particularly induced under conditions of cellular stress, heat, or toxins and conditions which lead to the accumulation of unfolded proteins in the ER.
      Product Information
      PresentationPurified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.
      ApplicationThis Anti-GRP78 Antibody, clone N88 is validated for use in western blotting, activity assay & flow cytometry for the detection of GRP78.
      Key Applications
      • Western Blotting
      • Activity Assay
      • Flow Cytometry
      Application NotesActivity Assay Analysis: This antibody accelerates tumor growth in a B16F1 melanoma Flank tumor model (de Ridder, G. G., et al. (2012). Melanoma Research. 22(3):225-235).
      Flow Cytometry Analysis: A representative lot detected GRP78 in B16F1 cells (de Ridder, G. G., et al. (2012). Melanoma Research. 22(3):225-235).
      Biological Information
      ImmunogenRecombinant protein corresponding to human GRP78.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HSPA5
      • GRP78
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~78 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected GRP78 in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-GRP78, clone N88 - Q2395227 Q2395227


      Reference overviewPub Med ID
      A murine monoclonal antibody directed against the carboxyl-terminal domain of GRP78 suppresses melanoma growth in mice.
      de Ridder, Gustaaf G, et al.
      Melanoma Res., 22: 225-35 (2012) 2012

      Show Abstract
      22495669 22495669

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      Life Science Research > Antibodies and Assays > Primary Antibodies