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MABS87 | Anti-GPR177 Antibody, clone YJ5

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, ICC M Purified Monoclonal Antibody
      Catalogue NumberMABS87
      DescriptionAnti-GPR177 Antibody, clone YJ5
      Alternate Names
      • Protein wntless homolog
      • Integral membrane protein GPR177
      • Protein evenness interrupted homolog
      • Putative NF-kappa-B-activating protein 373
      • WLS
      • EVI
      Background InformationGpr177 (WLS, EVI), the mouse ortholog of Drosophila WIs, is expressed during mouse embryogenesis. It is essential for the patterning of the anterior-posterior axis during mammalian development and is activated by â-catenin and LEF/TCF-dependent transcription. Upon GPR177 activation, Wnt binds with and modifies the cellular distribution of GPR177, leading to a feedback regulatory mechanism involving Wnt expression and signaling.
      Product Information
      • HeLa cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-GPR177 Antibody, clone YJ5 is an antibody against GPR177 for use in WB & IC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:50 dilution from a representative lot detected GPR177 in HeLa cells (Prof. D.M. Virshup, Program in Cancer and Stem Cell Biology, Duke-NUS Graduate Medical School.) (Also reference Coombs, G.S., et al. (2010). J Cell Sci. 123(19):3357-3367).
      Biological Information
      ImmunogenHistidine-tagged recombinant protein corresponding to human GPR177.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • WLS
      • C1orf139
      • GPR177
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins. Plays also an important role in establishment of the anterior-posterior body axis formation during development.

      SUBUNIT STRUCTURE: Interacts with WNT3A. Interacts with WNT1, WNT3 and WNT5A.

      SUBCELLULAR LOCATION: Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein.
      Molecular Weight~62 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: A 1:5,000 dilution of this antibody detected GPR177 in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-GPR177, clone YJ5 - 2145084 2145084
      Anti-GPR177, clone YJ5 - 2375595 2375595
      Anti-GPR177, clone YJ5 - 2064322 2064322
      Anti-GPR177, clone YJ5 - 2225191 2225191
      Anti-GPR177, clone YJ5 - 2297300 2297300
      Anti-GPR177, clone YJ5 - NRG1945628 NRG1945628
      Anti-GPR177, clone YJ5 -2490717 2490717
      Anti-GPR177, clone YJ5 -2576388 2576388
      Anti-GPR177, clone YJ5 -2604839 2604839
      Anti-GPR177, clone YJ5 -2619694 2619694
      Anti-GPR177, clone YJ5 -2689666 2689666
      Anti-GPR177, clone YJ5 -2800175 2800175


      Reference overviewPub Med ID
      Induction and transport of Wnt 5a during macrophage-induced malignant invasion is mediated by two types of extracellular vesicles.
      Menck, K; Klemm, F; Gross, JC; Pukrop, T; Wenzel, D; Binder, C
      Oncotarget 4 2057-66 2013

      Show Abstract
      24185202 24185202
      A uniform human Wnt expression library reveals a shared secretory pathway and unique signaling activities.
      Najdi, R; Proffitt, K; Sprowl, S; Kaur, S; Yu, J; Covey, TM; Virshup, DM; Waterman, ML
      Differentiation; research in biological diversity 84 203-13 2012

      Show Abstract
      22784633 22784633
      WLS-dependent secretion of WNT3A requires Ser209 acylation and vacuolar acidification.
      Coombs, Gary S, et al.
      J. Cell. Sci., 123: 3357-67 (2010) 2010

      Show Abstract
      20826466 20826466
      Reciprocal regulation of Wnt and Gpr177/mouse Wntless is required for embryonic axis formation.
      Fu, Jiang, et al.
      Proc. Natl. Acad. Sci. U.S.A., 106: 18598-603 (2009) 2009

      Show Abstract
      19841259 19841259
      Wntless, a conserved membrane protein dedicated to the secretion of Wnt proteins from signaling cells.
      Bänziger, Carla, et al.
      Cell, 125: 509-22 (2006) 2006

      Show Abstract
      16678095 16678095


      Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.