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MABS132 | Anti-GLUT1 (CT) Antibody, clone 5B12.3

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R WB, IHC M Purified Monoclonal Antibody
      Catalogue NumberMABS132
      DescriptionAnti-GLUT1 (CT) Antibody, clone 5B12.3
      Alternate Names
      • Solute carrier family 2 facilitated glucose transporter member 1
      • Glucose transporter type 1,erythrocyte/brain
      • GLUT-1
      • HepG2 glucose transporter
      Background InformationGLUTs (Glucose transporters) are a family of integral membrane proteins that facilitative glucose uptake by cells. They are integral membrane glycoproteins involved in transporting glucose into most cells. Seven types of glucose transport carrier proteins, designated as GLUT 1 to 7, facilitate glucose transport across the cell membrane. Molecular cloning of glucose transporters have identified a family of closely related genes that encode at least 7 proteins exhibiting high degree of amino acid homology (45% to 65%), all in the molecular weight range of 40 to 60 kDa. GLUT1 functions in constitutive or basal glucose uptake in adult tissues and is the predominant glucose transporter in embryonic and fetal tissues. It has two forms, a 55 kDa and a 45 kDa. The 55 kDa form of GLUT1 regulates import of glucose from blood to brain across the endothelial cells of the blood-brain barrier (BBB), whereas the 45 kDa form of GLUT1 predominantly regulates nonvascular glial glucose uptake.
      Product Information
      • NGF treated PC-12 cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-GLUT1 (C-terminus) Antibody, clone 5B12.3 is an antibody against GLUT1 (C-terminus) for use in Western Blotting & IHC.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected GLUT1 in rat cerebellum and human cerebellum tissues.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human GLUT1.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes GLUT1 at the C-terminus.
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SLC2A1
      • GLUT1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~54 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in NGF treated PC-12 cell lysate.

      Western Blot Analysis: 0.1 µg/mL of this antibody detected GLUT1 in 10 µg of NGF treated PC-12 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-GLUT1 (C-terminus), clone 5B12.3 2487740
      Anti-GLUT1 (C-terminus), clone 5B12.3 - 2119025 2119025
      Anti-GLUT1 (C-terminus), clone 5B12.3 - 2424159 2424159
      Anti-GLUT1 (C-terminus), clone 5B12.3 - 2430566 2430566
      Anti-GLUT1 (C-terminus), clone 5B12.3 - 2211978 2211978
      Anti-GLUT1 (C-terminus), clone 5B12.3 - 2298449 2298449
      Anti-GLUT1 (C-terminus), clone 5B12.3 - Q2037262 Q2037262
      Anti-GLUT1 (C-terminus), clone 5B12.3 -2505488 2505488
      Anti-GLUT1 (C-terminus), clone 5B12.3 -2605480 2605480
      Anti-GLUT1 (C-terminus), clone 5B12.3 -2614684 2614684
      Anti-GLUT1 (C-terminus), clone 5B12.3 -2634666 2634666
      Anti-GLUT1 (C-terminus), clone 5B12.3 -2646509 2646509
      Anti-GLUT1 (C-terminus), clone 5B12.3 -2683312 2683312
      Anti-GLUT1 (C-terminus), clone 5B12.3 -2766465 2766465


      Reference overviewPub Med ID
      Endotoxin-induced inflammation down-regulates L-type amino acid transporter 1 (LAT1) expression at the blood-brain barrier of male rats and mice.
      Wittmann, G; Mohácsik, P; Balkhi, MY; Gereben, B; Lechan, RM
      Fluids and barriers of the CNS 12 21 2015

      Show Abstract
      26337286 26337286
      Impact of cigarette smoke extract and hyperglycemic conditions on blood-brain barrier endothelial cells.
      Prasad, S; Sajja, RK; Park, JH; Naik, P; Kaisar, MA; Cucullo, L
      Fluids and barriers of the CNS 12 18 2015

      Show Abstract
      26206552 26206552
      PET Demonstrates Functional Recovery after Treatment by Danhong Injection in a Rat Model of Cerebral Ischemic-Reperfusion Injury.
      Wang, Z; Song, F; Li, J; Zhang, Y; He, Y; Yang, J; Zhou, H; Zhao, T; Fu, W; Xing, P; Wan, H; Tian, M; Zhang, H
      Evidence-based complementary and alternative medicine : eCAM 2014 430757 2014

      Show Abstract
      24707308 24707308

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