ST1543 Anti-GHR Mouse mAb (3A12)

ST1543
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      ST1543-100UG
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          100 μg
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          Description
          OverviewRecognizes the GHR protein in HeLa cells.
          Catalogue NumberST1543
          Brand Family Calbiochem®
          SynonymsAnti-Growth Hormone Receptor
          Application Data
          Detection of human GHR by immunoblotting. Sample: Partial recombinant human GHR (immunogen). Primary antibody: Anti-GHR Mouse mAb (3A12) (Cat. No. ST1543) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human GHR by Proximity Ligation Analysis of protein-protein interaction using immunocytochemistry. Sample: HeLa cells. Primary antibodies: Anti-STAT5 (1:200) and Anti-GHR Mouse mAb (3A12) (1:50). Detection: fluorescence with DAPI counterstain.
          References
          Product Information
          FormLiquid
          FormulationIn PBS, pH 7.2.
          Positive controlHeLa cells
          PreservativeNone
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunocytochemistry
          Application NotesELISA (see coments)
          Immunoblotting (1-5 µg/ml)
          Immunocytochemistry (1:50)
          Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          ImmunogenA recombinant polypeptide corresponding to amino acids 19-119 of human GHR, expressed as a GST fusion protein
          Clone3A12
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Regulatory Review
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-GHR Mouse mAb (3A12) SDS

          Title

          Safety Data Sheet (SDS) 

          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision08-November-2010 RFH
          SynonymsAnti-Growth Hormone Receptor
          ApplicationELISA (see coments)
          Immunoblotting (1-5 µg/ml)
          Immunocytochemistry (1:50)
          Application Data
          Detection of human GHR by immunoblotting. Sample: Partial recombinant human GHR (immunogen). Primary antibody: Anti-GHR Mouse mAb (3A12) (Cat. No. ST1543) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human GHR by Proximity Ligation Analysis of protein-protein interaction using immunocytochemistry. Sample: HeLa cells. Primary antibodies: Anti-STAT5 (1:200) and Anti-GHR Mouse mAb (3A12) (1:50). Detection: fluorescence with DAPI counterstain.
          DescriptionPurified mouse monoclonal antibody. Recognizes the GHR protein.
          BackgroundGHR is a protein that is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. A common alternate allele of this gene, called GHRd3, lacks exon three and has been well-characterized. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature.
          HostMouse
          ImmunogenA recombinant polypeptide corresponding to amino acids 19-119 of human GHR, expressed as a GST fusion protein
          Clone3A12
          IsotypeIgG2a
          Specieshuman
          Positive controlHeLa cells
          FormLiquid
          FormulationIn PBS, pH 7.2.
          PreservativeNone
          CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Regulatory Review