A part of MilliporeSigma

MABD117 | Anti-GATA Antibody3, clone 7B5

100 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
Request Pricing
Limited AvailabilityLimited Availability
Limited Quantities Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers


      Contact Customer Service

      Click To Print This Page


      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, IHC M Ascites Monoclonal Antibody
      Catalogue NumberMABD117
      DescriptionAnti-GATA Antibody3, clone 7B5
      Alternate Names
      • Trans-acting T-cell-specific transcription factor GATA-3
      • GATA-binding factor 3
      Background InformationGATA-binding factor 3 (GATA3), also known as Trans-acting T-cell-specific transcription factor GATA-3, is a transcription factor containing a zinc finger domain. GATA3 is primarily expressed in T cells, where it thought to participate in T-cell receptor gene activation through binding to enhancers. Analysis of conditional GATA3 knockout mice confirmed the critical role of GATA3 in helper T cell differentiation and proliferation. Cells expressing GATA3 develop the profile of Th2 cells, secreting IL-4, IL-5 and IL-13. Binding of GATA3 in the regulatory regions of the genes of these cytokines alters chromatin structure, increasing accessibility to other transcription factors. T cell receptor activation activates NFAT and other transcription factors that cooperate with GATA3 in inducing Th2 cell differentiation. Blocking GATA3 action has been suggested as a therapeutic strategy to treat asthma. GATA3 has also been implicated in developmental processes such as the development of the inner ear.
      Product Information
      • GATA3-hIgGFc transfected HEK293 cell lysate
      PresentationMouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
      ApplicationDetect GATA using this mouse monoclonal antibody, Anti-GATA Antibody3, clone 7B5 validated for use in western blotting & IHC.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected GATA3 in human small intestine tissue.

      Optimal working dilutions must be determined by end user.

      Biological Information
      ImmunogenPurified recombinant fragment of human GATA3 expressed in E. Coli.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • GATA3
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~75 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in GATA3-hIgGFc transfected HEK293 cell lysate.

      Western Blot Analysis: A 1:500-2000 dilution of this antibody detected GATA3 in GATA3-hIgGFc transfected HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information

      Related Products & Applications

      Related Products

      Catalog Number Description  
      09-076 Anti-GATA3 Antibody Show Pricing & Availability
      MABE271 Anti-GATA3 Antibody, clone 3F1.1 Show Pricing & Availability
      MABE444 Anti-GATA-binding factor 3 Antibody, clone 7E8.1 Show Pricing & Availability

      View Gene Related Products for this Target


      Life Science Research > Antibodies and Assays > Primary Antibodies