CB1001 Anti-GAPDH Mouse mAb (6C5)

CB1001
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      Ca, Ch, F, Fg, H, M, Porcine, Rb, R M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      CB1001-500UG
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          Plastic ampoule 500 μg
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          Description
          OverviewRecognizes the ~146 kDa GAPDH protein under non-reducing conditions and the ~36 kDa monomeric subunits under reducing conditions.
          Catalogue NumberCB1001
          Brand Family Calbiochem®
          SynonymsAnti-Glyceraldehyde-3-Phosphate Dehydrogenase
          Application Data
          Detection of rat GAPDH by immunocytochemistry. Sample: A-10 cell line (rat aortic smooth muscle cells). Primary antibody: Anti-GAPDH Mouse mAb (6C5) (Cat. No. CB1001) (assay dependent of Primary Antibody). Detection: fluorescence.

          Detection of GAPDH by immunoblotting. Samples: Tissue exctract from isolated human GAPDH (lane 1), human heart (lane 2), pig heart (lane 3), goat heart (lane 4), bovine heart (lane 5), dog heart (lane 6), mouse heart (lane 7), rat heart (lane 8), rabbit heart (lane 9), and duck heart (lane 10). Primary antibody: Anti-GAPDH Mouse mAb (6C5) (Cat. No. CB1001) (1 µg/ml). Detection: chemiluminescence.
          References
          Product Information
          FormLiquid
          FormulationIn PBS, pH 7.4.
          Positive controlRat skeletal muscle
          Preservative≤0.1% sodium azide
          Applications
          Application ReferencesImmunoblotting Gagarin, D., et al. 2005. J. Mol. Cell. Card. 39, 453. Yang, Z., et al. 2007. J. Vasc. Res. 44, 483.
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunocytochemistry
          Application NotesELISA (see comments)
          Immunoblotting (1-10 µg/ml; see application references)
          Immunocytochemistry (see comments)
          Application CommentsUseful as a loading or positive control since GAPDH is expressed in all cells. Also reported to work for ELISA and immunocytochemistry. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenpurified, rabbit muscle
          ImmunogenRabbit
          Clone6C5
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Canine
          • Chicken
          • Fish
          • Frog
          • Human
          • Mouse
          • Porcine
          • Rabbit
          • Rat
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Highly Toxic
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-GAPDH Mouse mAb (6C5) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-GAPDH Mouse mAb (6C5) Certificates of Analysis

          TitleLot Number
          CB1001
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision30-November-2007 JSW
          SynonymsAnti-Glyceraldehyde-3-Phosphate Dehydrogenase
          ApplicationELISA (see comments)
          Immunoblotting (1-10 µg/ml; see application references)
          Immunocytochemistry (see comments)
          Application Data
          Detection of rat GAPDH by immunocytochemistry. Sample: A-10 cell line (rat aortic smooth muscle cells). Primary antibody: Anti-GAPDH Mouse mAb (6C5) (Cat. No. CB1001) (assay dependent of Primary Antibody). Detection: fluorescence.

          Detection of GAPDH by immunoblotting. Samples: Tissue exctract from isolated human GAPDH (lane 1), human heart (lane 2), pig heart (lane 3), goat heart (lane 4), bovine heart (lane 5), dog heart (lane 6), mouse heart (lane 7), rat heart (lane 8), rabbit heart (lane 9), and duck heart (lane 10). Primary antibody: Anti-GAPDH Mouse mAb (6C5) (Cat. No. CB1001) (1 µg/ml). Detection: chemiluminescence.
          DescriptionProtein A purified mouse monoclonal antibody. Recognizes the ~146 kDa GAPDH protein under non-reducing conditions and the ~36 kDa monomeric subunits under reducing conditions.
          BackgroundGlyceraldehyde-3-phosphate dehydrogenase (GAPDH) is one of the key enzymes involved in glycolysis. GAPDH is comprised of four ~36 kDa subunits with a total molecular weight of ~146 kDa. Besides functioning as a glycolytic enzyme in cytoplasm, mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. Recently, findings have been made concerning the role of GAPDH in differentpathologies including prostate cancer progression, programmed neuronal cell death, and age-related neuronal diseases such as Alzheimer’s Disease and Huntington’s Disease. While GAPDH is constitutively expressed in almost all tissues at high levels, there are some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types.
          HostMouse
          Immunogen speciesRabbit
          Immunogenpurified, rabbit muscle
          Clone6C5
          IsotypeIgG₁
          Speciesnot bovine, canine, chicken, fish, frog, human, mouse, porcine, rabbit, rat, not yeast
          Positive controlRat skeletal muscle
          FormLiquid
          FormulationIn PBS, pH 7.4.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsUseful as a loading or positive control since GAPDH is expressed in all cells. Also reported to work for ELISA and immunocytochemistry. Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
          Toxicity Highly Toxic
          Application referencesImmunoblotting Gagarin, D., et al. 2005. J. Mol. Cell. Card. 39, 453. Yang, Z., et al. 2007. J. Vasc. Res. 44, 483.