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ABS16 | Anti-GAPDH Antibody

ABS16
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, B, Gp WB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberABS16
      DescriptionAnti-GAPDH Antibody
      Alternate Names
      • glyceraldehyde 3-phosphate dehydrogenase
      • aging-associated gene 9 protein
      • glyceraldehyde-3-phosphate dehydrogenase, GAPDH
      • G3PDH
      • GAPD
      • OK/SW-cl.12
      • MGC88685
      • G3PD
      • EC 1.2.1.12
      Background InformationGlyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in moderately high levels in almost all tissues. As a 'house-keeping' enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic action in metabolism it is also involved in the initial stages of apoptosis or oxidative stress response where GAPDH is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH has also been found to bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders including the beta-amyloid precursor protein and the huntingtin protein where decreased function of GAPDH is associated with Alzheimer's and Huntington's disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP).
      References
      Product Information
      FormatAffinity Purified
      Control
      • HEK293 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody is popular affinity purified polyclonal antibody. This antibody is supported by peer reviewed publications and reliably detects Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) is validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the C-terminus of human GAPDH.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes GAPDH at the C-terminus.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Bovine
      • Guinea Pig
      Species Reactivity NoteDemonstrated to react with human, mouse, and rat. Predicted to react with bovine and guinea pig based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq].
      Gene Symbol
      • GAPDH
      • G3PDH
      • GAPD
      • G3PD
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway.

      SIZE: 335 amino acids; 36053 Da

      SUBUNIT: Homotetramer. Binds PRKCI.

      SUBCELLULAR LOCATION: Cytoplasm. Note=Postnuclear and Perinuclear regions. Membrane.

      SIMILARITY: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
      Molecular Weight ~36kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HEK293 cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected GAPDH on 10 µg of HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-GAPDH - 2128266 2128266
      Anti-GAPDH - 2384012 2384012
      Anti-GAPDH - 2459656 2459656
      Anti-GAPDH - 2059942 2059942
      Anti-GAPDH - 2201477 2201477
      Anti-GAPDH - 2272157 2272157
      Anti-GAPDH - 2491262 2491262
      Anti-GAPDH - 2535325 2535325
      Anti-GAPDH - NG1871260 NG1871260
      Anti-GAPDH - NG1881293 NG1881293
      Anti-GAPDH - NG1897885 NG1897885
      Anti-GAPDH - NG1906432 NG1906432
      Anti-GAPDH - NRG1840244 NRG1840244
      Anti-GAPDH -2552651 2552651
      Anti-GAPDH -2605479 2605479
      Anti-GAPDH -2625354 2625354
      Anti-GAPDH -2654766 2654766
      Anti-GAPDH -2707232 2707232
      Anti-GAPDH -2745933 2745933
      Anti-GAPDH -2794839 2794839

      References

      Reference overviewApplicationSpeciesPub Med ID
      Activation of peroxisome proliferator-activated receptor γ ameliorates monocrotaline-induced pulmonary arterial hypertension in rats.
      Xie, X; Wang, G; Zhang, D; Zhang, Y; Zhu, Y; Li, F; Li, S; Li, M
      Biomedical reports 3 537-542 2015

      Show Abstract
      26171162 26171162
      AduPARE1A and gemcitabine combined treatment trigger synergistic antitumor effects in pancreatic cancer through NF-κB mediated uPAR activation.
      Maliandi, MV; Mato-Berciano, A; Sobrevals, L; Roué, G; José, A; Fillat, C
      Molecular cancer 14 146 2015

      Show Abstract
      26227809 26227809
      Wnts enhance neurotrophin-induced neuronal differentiation in adult bone-marrow-derived mesenchymal stem cells via canonical and noncanonical signaling pathways.
      Tsai, HL; Deng, WP; Lai, WF; Chiu, WT; Yang, CB; Tsai, YH; Hwang, SM; Renshaw, PF
      PloS one 9 e104937 2014

      Show Abstract
      ImmunofluorescenceHuman25170755 25170755
      Comparison of the anti-prion mechanism of four different anti-prion compounds, anti-PrP monoclonal antibody 44B1, pentosan polysulfate, chlorpromazine, and U18666A, in prion-infected mouse neuroblastoma cells.
      Yamasaki, T; Suzuki, A; Hasebe, R; Horiuchi, M
      PloS one 9 e106516 2014

      Show Abstract
      25181483 25181483
      Receptor-interacting protein kinases modulate noise-induced sensory hair cell death.
      Zheng, HW; Chen, J; Sha, SH
      Cell death & disease 5 e1262 2014

      Show Abstract
      Western Blotting24874734 24874734
      Contribution of transcription factor, SP1, to the promotion of HB-EGF expression in defense mechanism against the treatment of irinotecan in ovarian clear cell carcinoma.
      Miyata, K; Yotsumoto, F; Nam, SO; Odawara, T; Manabe, S; Ishikawa, T; Itamochi, H; Kigawa, J; Takada, S; Asahara, H; Kuroki, M; Miyamoto, S
      Cancer medicine 3 1159-69 2014

      Show Abstract
      Western Blotting25060396 25060396
      Mitochondrial peroxiredoxin 3 regulates sensory cell survival in the cochlea.
      Chen, FQ; Zheng, HW; Schacht, J; Sha, SH
      PloS one 8 e61999 2013

      Show Abstract
      23626763 23626763
      The miR-183/Taok1 target pair is implicated in cochlear responses to acoustic trauma.
      Patel, M; Cai, Q; Ding, D; Salvi, R; Hu, Z; Hu, BH
      PloS one 8 e58471 2013

      Show Abstract
      23472202 23472202
      Traumatic noise activates Rho-family GTPases through transient cellular energy depletion.
      Chen, FQ; Zheng, HW; Hill, K; Sha, SH
      The Journal of neuroscience : the official journal of the Society for Neuroscience 32 12421-30 2012

      Show Abstract
      22956833 22956833
      Laminin 511 partners with laminin 332 to mediate directional migration of Madin-Darby canine kidney epithelial cells.
      Greciano, PG; Moyano, JV; Buschmann, MM; Tang, J; Lu, Y; Rudnicki, J; Manninen, A; Matlin, KS
      Molecular biology of the cell 23 121-36 2012

      Show Abstract
      22031290 22031290

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies