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MAB374-AF488 | Anti-GAPDH Antibody, clone 6C5, Alexa Fluor® 488 conjugate

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H ICC M AlexaFluor®488 Monoclonal Antibody
      Catalogue NumberMAB374-AF488
      DescriptionAnti-GAPDH Antibody, clone 6C5, Alexa Fluor® 488 conjugate
      Alternate Names
      • Glyceraldehyde-3-phosphate dehydrogenase
      • GAPDH
      • Peptidyl-cysteine S-nitrosylase GAPDH
      • GAPDH
      Background InformationGlyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Its binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP). As a 'house-keeping' enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic role in metabolism, it is also involved in the initial stages of apoptosis or oxidative stress response where it is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH can also bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders, including the beta-amyloid precursor protein and the Huntingtin protein where reduced function of GAPDH is associated with Alzheimer's and Huntington's disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity.
      Product Information
      PresentationPurified mouse conjugate antibody in buffer containing PBS 15 mg/ml BSA and 0.1% Sodium Azide.
      ApplicationThis Anti-GAPDH Antibody, clone 6C5, Alexa Fluor® 488 conjugate is validated for use in Immunocytochemistry for the detection of GAPDH.
      Key Applications
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GAPDH in NIH/3T3 cells.
      The unconjugated version of this antibody (Cat. No. MAB374) has been demonstrated to perform in WB, ELISA, ICC, IHC, and IP.
      Biological Information
      ImmunogenGlyceraldehyde-3-phosphate dehydrogenase from rabbit muscle.
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityGlyceraldehyde-3-phosphate dehydrogenase (GAPDH) from skeletal muscle. Antibody also recognizes cardiac GAPDH. GAPDH enzyme is detected in many non-muscle cells lines including HeLa, HCT-116 cells, U937 and THP-1 cells among others.
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman and Mouse. Predicted to react with Porcine, Canine, Cat, Rat, Rabbit, and Fish based on 100% sequence homology. It has been reported that this antibody does not react with GAPDH from E. coli.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GAPDH
      • GAPD
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight~38 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected GAPDH in A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information