AB5394 Anti-GABA B Receptor R2 Antibody

AB5394
50 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, Spd IHC Gp Serum Polyclonal Antibody
      Description
      Catalogue NumberAB5394
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-GABA B Receptor R2 Antibody
      References
      Product Information
      FormatSerum
      Control
      • Brain tissue
      PresentationGuinea pig Serum. Liquid. Contains no preservative.
      Applications
      ApplicationAnti-GABA B Receptor R2 Antibody detects level of GABA B Receptor R2 & has been published & validated for use in IH.
      Key Applications
      • Immunohistochemistry
      Application NotesImmunohistochemistry: 1:1,000-1:2,000 using a cyanine conjugated secondary antibody. Light 4% PFA fixation recommended. Permeabilization with 0.1-.2% triton in the block only; enzymatic detection can be used however substantially higher primary antibody dilutions will be necessary. Traditional formalin fixation is not recommended.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic peptide corresponding to the C-terminal amino acids of the rat and human GABAB-R2 receptor protein (Kaupman et al., 1998; White et al., 1998; Jones et al., 1998; Martin et al., 1999).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostGuinea Pig
      SpecificityRecognizes GABAB-R2 receptor. The antiserum stains nerve fibers and cell bodies in untreated animals. The staining pattern for the immunoreactive cell bodies obtained with the GABAB-R2 receptor antiserum corresponds to the pattern described using in situ hybridization with probes to rat GABAB-R2 receptor mRNA (Durkin et al., 1999). Strong GABAB-R2 receptor immunoreactivity is e.g. demonstrated in the Purkinje cells of the cerebellum, in the cerebral cortex, in the medial habelulae, in the spinal cord and in the brainstem. In individual Purkinje neurons, the immunoreactivity is preferentially observed in the periphery of the cells, most likely corresponding to the plasma membrane, but also in the entire dendritic tree extending into the molecular cell layer of the cerebellum.
      Species Reactivity
      • Rat
      • Spider
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryB-type receptors for the neurotransmitter GABA (gamma-aminobutyric acid) inhibit neuronal activity through G protein-coupled second-messenger systems, which regulate the release of neurotransmitters and the activity of ion channels and adenylyl cyclase. See GABBR1 (MIM 603540) for additional background information on GABA-B receptors.[supplied by OMIM]
      Gene Symbol
      • GABBR2
      • HRIHFB2099
      • OTTHUMP00000063797
      • GPRC3B
      • GPR51
      • HG20
      • Gb2
      • FLJ36928
      • GABA-B-R2
      • GABABR2
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O75899 # Receptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high- voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception.
      SIZE: 941 amino acids; 105821 Da
      SUBUNIT: Heterodimer of GABA-B-R1 and GABA-B-R2. Neither of which is effective on its own and homodimeric assembly does not seem to happen. Interacts with ATF4 via its C-terminal region.
      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Note=Moreover coexpression of GABA-B-R1 and GABA-B-R2 appears to be a prerequisite for maturation and transport of GABA-B-R1 to the plasma membrane.
      TISSUE SPECIFICITY: Highly expressed in brain, especially in cerebral cortex, thalamus, hippocampus, frontal, occipital and temporal lobe, occipital pole and cerebellum, followed by corpus callosum, caudate nucleus, spinal cord, amygdala and medulla. Weakly expressed in heart, testis and skeletal muscle.
      DOMAIN: SwissProt: O75899 Alpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 1.
      SIMILARITY: Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 6 months at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewApplicationPub Med ID
      Altered emotionality and neuronal excitability in mice lacking KCTD12, an auxiliary subunit of GABAB receptors associated with mood disorders.
      Cathomas, F; Stegen, M; Sigrist, H; Schmid, L; Seifritz, E; Gassmann, M; Bettler, B; Pryce, CR
      Translational psychiatry  5  e510  2015

      Show Abstract
      25689571 25689571
      GABAB receptor phosphorylation regulates KCTD12-induced K⁺ current desensitization.
      Adelfinger, L; Turecek, R; Ivankova, K; Jensen, AA; Moss, SJ; Gassmann, M; Bettler, B
      Biochemical pharmacology  91  369-79  2014

      Show Abstract
      25065880 25065880
      Opposite Effects of KCTD Subunit Domains on GABAB Receptor-mediated Desensitization.
      Riad Seddik,Stefan P Jungblut,Olin K Silander,Mathieu Rajalu,Thorsten Fritzius,Val Besseyrias,Val Jacquier,Bernd Fakler,Martin Gassmann,Bernhard Bettler
      The Journal of biological chemistry  287  2012

      Show Abstract
      23035119 23035119
      Presynaptic GABA(B) receptors decrease neurotransmitter release in vestibular nuclei neurons during vestibular compensation.
      Shao, M; Reddaway, R; Hirsch, JC; Peusner, KD
      Neuroscience  223  333-54  2012

      Show Abstract
      22871524 22871524
      The level and distribution of the GABA(B)R1 and GABA(B)R2 receptor subunits in the rat's inferior colliculus.
      Jamal, L; Khan, AN; Butt, S; Patel, CR; Zhang, H
      Frontiers in neural circuits  6  92  2012

      Show Abstract
      23189044 23189044
      ?-Aminobutyric acid type B receptor changes in the rat striatum and substantia nigra following intrastriatal quinolinic acid lesions.
      Letaïef Rekik,Véronique Daguin-Nerrière,Jean-Yves Petit,Philippe Brachet
      Journal of neuroscience research  89  2011

      Show Abstract
      21290407 21290407
      Functional expression of GABAB receptors in airway epithelium.
      Kentaro Mizuta, Yoko Osawa, Fumiko Mizuta, Dingbang Xu, Charles W Emala, Kentaro Mizuta, Yoko Osawa, Fumiko Mizuta, Dingbang Xu, Charles W Emala
      American journal of respiratory cell and molecular biology  39  296-304  2008

      Show Abstract Full Text Article
      18403780 18403780
      Developing oligodendrocytes express functional GABA(B) receptors that stimulate cell proliferation and migration.
      Karen Luyt, Timothy P Slade, Jienchi J Dorward, Claire F Durant, Yue Wu, Ryuichi Shigemoto, Stuart J Mundell, Anikó Váradi, Elek Molnár
      Journal of neurochemistry  100  822-40  2007

      Show Abstract
      17144904 17144904
      GABA receptor-mediated effects in the peripheral nervous system: A cross-interaction with neuroactive steroids.
      Magnaghi, V. et al.
      J Mol Neurosci. , 28:89-102 (2006)  2006

      16632878 16632878
      Differential expression of gamma-aminobutyric acid type B receptor subunit mRNAs in the developing nervous system and receptor coupling to adenylyl cyclase in embryonic neurons.
      Stella C Martin, Janine L Steiger, María Clara Gravielle, Helen R Lyons, Shelley J Russek, David H Farb
      The Journal of comparative neurology  473  16-29  2004

      Show Abstract
      15067715 15067715

      Data Sheet

      Title
      GUINEA PIG ANTI-GABAB-R2 RECEPTOR

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies