AB175 Anti-GABA Antibody

AB175
50 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, A IH(P), ELISA Gp Serum Polyclonal Antibody
      Description
      Catalogue NumberAB175
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-GABA Antibody
      Background InformationGABA (gamma-aminobutyric acid) is the major inhibitory neurotransmitter in the central nervous system that inhibits the generation of the action potential of the neuron. It is involved in the pathogenesis of certain neurological and psychiatric disorders. GABA is produced from glutamic acid in a reaction catalyzed by glutamic acid decarboxylase. In this reaction pyridoxal phosphate serves as a co-factor. GABA interacts with the GABAA and GABAB receptors, which are widely distributed throughout the nervous system and in a variety of cell types. They differ in their pharmacological, electrophysiological, and biochemical properties. GABAA-receptor complex mediates an increase in membrane conductance with an equilibrium potential near the resting level of −70 mV. This conductance increase often is accompanied by a membrane hyperpolarization, which later results in a reduction in the probability of action potential initiation. The reduction in membrane resistance is accomplished by the GABA-dependent facilitation of Cl− ion influx. GABAB receptors are coupled indirectly to K+ channels. When activated, GABAB receptors reduce Calcium ion conductance and inhibit cAMP production via intracellular mechanisms mediated by G-proteins. GABAB receptors are known to mediate both postsynaptic and presynaptic inhibition.
      References
      Product Information
      FormatSerum
      Control
      • Rat Brain tissue
      PresentationGuinea Pig polyclonal BSA-depleted antiserum with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-GABA Antibody is validated for use in Immunohistochemistry (Paraffin) and Enzyme Immunoassay (ELISA) for the detection of GABA.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Enzyme Immunoassay (ELISA)
      Application NotesImmunohistochemistry Analysis: A 1:500 dilution from a representative lot detected GABA in rat brain tissue.

      ELISA Analysis: A representative lot from an independent laboratory detected GABA in a competitive ELISA.

      Immunohistochemistry Protocol:

      Tissues fixed with 4% paraformaldehyde and 0-0.5% glutaraldehyde gives good results. Glutaraldehyde is required for antibody reactivity.

      1) Tissue is fixed with 4% paraformaldehyde, 0-0.5% glutaraldehyde, 0.5% potassium dichromate in 0.1M phosphate buffer at pH 6.5.

      2) Tissue is post-fixed overnight, vibratome sectioned in 50 mm and incubated in 0.05M Tris buffer, pH 6.5 for three hours.

      3) Sections are incubated for 18-24 hours in AB175 diluted in PBS containing 0.1% sodium azide, 0.2% Triton X-100 and 1% normal goat serum.

      4) Fluorescein conjugated antibody or ABC system may be used as the secondary reagent.

      Note: Without colchicine pretreatment well-stained cell bodies are visible in the cerebral cortex, cerebrallar cortex, superior colliculus and some brainstem raphe. With colchicine pretreatment, additional cell body staining is present in the interpeduncular nucleus and the dorsal column nuclei.
      Biological Information
      ImmunogenBSA-conjugated GABA-Glutaraldehyde
      ConcentrationVariable
      HostGuinea Pig
      SpecificityRecognizes GABA. Staining was blocked by preabsorbing with 100 μM GABA conjugated to glutaraldehyde. 500 μM of similar conjugations of glutamic acid, glutamate and taurine failed to block staining.
      Species Reactivity
      • Rat
      • All
      Species Reactivity NoteDemonstrated to react with Rat. GABA is a neurotransmitter synthesized in all species, and is therefore expected to react to all species.
      Antibody TypePolyclonal Antibody
      Purification MethodDepleted serum
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in rat brain tissue.

      Immunohistochemistry Analysis: A 1:500 dilution of this antibody detected GABA in rat brain tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-GABAANTI-GABA
      Anti-GABA - 23989122398912
      Anti-GABA - 21667292166729
      Anti-GABA - 21812722181272
      Anti-GABA - 22626842262684
      Anti-GABA - 23325482332548
      Anti-GABA - 25576532557653
      Anti-GABA - 30648303064830
      Anti-GABA - 31539693153969
      Anti-GABA -24890132489013

      References

      Reference overviewApplicationSpeciesPub Med ID
      Mapping synapses by conjugate light-electron array tomography.
      Collman, F; Buchanan, J; Phend, KD; Micheva, KD; Weinberg, RJ; Smith, SJ
      The Journal of neuroscience : the official journal of the Society for Neuroscience  35  5792-807  2015

      Show Abstract
      25855189 25855189
      Inner retinal change in a novel rd1-FTL mouse model of retinal degeneration.
      Greferath, U; Anderson, EE; Jobling, AI; Vessey, KA; Martinez, G; de Iongh, RU; Kalloniatis, M; Fletcher, EL
      Frontiers in cellular neuroscience  9  293  2015

      Show Abstract
      26283925 26283925
      Tonic inhibition in dentate gyrus impairs long-term potentiation and memory in an Alzheimer's [corrected] disease model.
      Wu, Z; Guo, Z; Gearing, M; Chen, G
      Nature communications  5  4159  2014

      Show Abstract
      Immunohistochemistry24923909 24923909
      PDE9A is expressed in the inner retina and contributes to the normal shape of the photopic ERG waveform.
      Dhingra, A; Tummala, SR; Lyubarsky, A; Vardi, N
      Frontiers in molecular neuroscience  7  60  2014

      Show Abstract
      25018695 25018695
      Ring finger protein 34 (RNF34) interacts with and promotes γ-aminobutyric acid type-A receptor degradation via ubiquitination of the γ2 subunit.
      Jin, H; Chiou, TT; Serwanski, DR; Miralles, CP; Pinal, N; De Blas, AL
      The Journal of biological chemistry  289  29420-36  2014

      Show Abstract
      25193658 25193658
      Neural circuit interactions between the dorsal raphe nucleus and the lateral hypothalamus: an experimental and computational study.
      Jalewa, J; Joshi, A; McGinnity, TM; Prasad, G; Wong-Lin, K; Hölscher, C
      PloS one  9  e88003  2014

      Show Abstract
      24516577 24516577
      Age- and light-dependent development of localised retinal atrophy in CCL2(-/-)CX3CR1(GFP/GFP) mice.
      Chen, M; Hombrebueno, JR; Luo, C; Penalva, R; Zhao, J; Colhoun, L; Pandi, SP; Forrester, JV; Xu, H
      PloS one  8  e61381  2013

      Show Abstract
      23637822 23637822
      Survey on amacrine cells coupling to retrograde-identified ganglion cells in the mouse retina.
      Pang, JJ; Paul, DL; Wu, SM
      Investigative ophthalmology & visual science  54  5151-62  2013

      Show Abstract
      23821205 23821205
      Anatomical characterization of a rabbit cerebellar eyeblink premotor pathway using pseudorabies and identification of a local modulatory network in anterior interpositus.
      Gonzalez-Joekes, J; Schreurs, BG
      The Journal of neuroscience : the official journal of the Society for Neuroscience  32  12472-87  2012

      Show Abstract
      Immunohistochemistry22956838 22956838
      Rod and cone pathway signalling is altered in the P2X7 receptor knock out mouse.
      Vessey, KA; Fletcher, EL
      PloS one  7  e29990  2012

      Show Abstract
      22253851 22253851

      Data Sheet

      Title
      GUINEA PIG ANTI-GABA POLYCLONAL ANTIBODY