A part of MilliporeSigma

AB175 | Anti-GABA Antibody

50 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, A IH(P), ELISA Gp Serum Polyclonal Antibody
      Catalogue NumberAB175
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-GABA Antibody
      Background InformationGABA (g-aminobutyric acid) is the major inhibitory neurotransmitter in the central nervous system that is involved in the pathogenesis of certain neurological and psychiatric disorders. GABA is produced from glutamic acid in a reaction catalyzed by glutamic acid decarboxylase. GABA interacts with the GABAA and GABAB receptors, which are widely distributed throughout the nervous system and in a variety of cell types. The immunolocalization, neuronal distribution, and vesicular compartmentalization of neurotransmitter molecules and their precursors are important for understanding the modulation, regulation, and function of neurotransmitters in the nervous system.
      Product Information
      • Rat Brain tissue
      PresentationPurified guinea pig polyclonal with 0.05% sodium azide.
      ApplicationThis Anti-GABA Antibody is validated for use in Immunohistochemistry (Paraffin) and Enzyme Immunoassay (ELISA) for the detection of GABA.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Enzyme Immunoassay (ELISA)
      Application NotesImmunohistochemistry Analysis: A 1:500 dilution from a representative lot detected GABA in rat brain tissue.

      ELISA Analysis: A representative lot from an independent laboratory detected GABA in a competitive ELISA.

      Immunohistochemistry Protocol:

      Tissues fixed with 4% paraformaldehyde and 0-0.5% glutaraldehyde gives good results. Glutaraldehyde is required for antibody reactivity.

      1) Tissue is fixed with 4% paraformaldehyde, 0-0.5% glutaraldehyde, 0.5% potassium dichromate in 0.1M phosphate buffer at pH 6.5.

      2) Tissue is post-fixed overnight, vibratome sectioned in 50 mm and incubated in 0.05M Tris buffer, pH 6.5 for three hours.

      3) Sections are incubated for 18-24 hours in AB175 diluted in PBS containing 0.1% sodium azide, 0.2% Triton X-100 and 1% normal goat serum.

      4) Fluorescein conjugated antibody or ABC system may be used as the secondary reagent.

      Note: Without colchicine pretreatment well-stained cell bodies are visible in the cerebral cortex, cerebrallar cortex, superior colliculus and some brainstem raphe. With colchicine pretreatment, additional cell body staining is present in the interpeduncular nucleus and the dorsal column nuclei.
      Biological Information
      ImmunogenBSA-conjugated GABA-Glutaraldehyde
      HostGuinea Pig
      SpecificityRecognizes GABA. Staining was blocked by preabsorbing with 100 μM GABA conjugated to glutaraldehyde. 500 μM of similar conjugations of glutamic acid, glutamate and taurine failed to block staining.
      Species Reactivity
      • Rat
      • All
      Species Reactivity NoteDemonstrated to react with Rat. GABA is a neurotransmitter synthesized in all species, and is therefore expected to react to all species.
      Antibody TypePolyclonal Antibody
      Purification MethodDepleted serum
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in rat brain tissue.

      Immunohistochemistry Analysis: A 1:500 dilution of this antibody detected GABA in rat brain tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-GABA - 2398912 2398912
      Anti-GABA - 2166729 2166729
      Anti-GABA - 2181272 2181272
      Anti-GABA - 2262684 2262684
      Anti-GABA - 2332548 2332548
      Anti-GABA - 2557653 2557653
      Anti-GABA -2489013 2489013
      Anti-GABA -2499671 2499671
      Anti-GABA -2688582 2688582
      Anti-GABA -2716204 2716204
      Anti-GABA -2745622 2745622

      References | 30 Available | See All References

      Reference overviewApplicationSpeciesPub Med ID
      Mapping synapses by conjugate light-electron array tomography.
      Collman, F; Buchanan, J; Phend, KD; Micheva, KD; Weinberg, RJ; Smith, SJ
      The Journal of neuroscience : the official journal of the Society for Neuroscience 35 5792-807 2015

      Show Abstract
      25855189 25855189
      Inner retinal change in a novel rd1-FTL mouse model of retinal degeneration.
      Greferath, U; Anderson, EE; Jobling, AI; Vessey, KA; Martinez, G; de Iongh, RU; Kalloniatis, M; Fletcher, EL
      Frontiers in cellular neuroscience 9 293 2015

      Show Abstract
      26283925 26283925
      Tonic inhibition in dentate gyrus impairs long-term potentiation and memory in an Alzheimer's [corrected] disease model.
      Wu, Z; Guo, Z; Gearing, M; Chen, G
      Nature communications 5 4159 2014

      Show Abstract
      Immunohistochemistry24923909 24923909
      PDE9A is expressed in the inner retina and contributes to the normal shape of the photopic ERG waveform.
      Dhingra, A; Tummala, SR; Lyubarsky, A; Vardi, N
      Frontiers in molecular neuroscience 7 60 2014

      Show Abstract
      25018695 25018695
      Ring finger protein 34 (RNF34) interacts with and promotes γ-aminobutyric acid type-A receptor degradation via ubiquitination of the γ2 subunit.
      Jin, H; Chiou, TT; Serwanski, DR; Miralles, CP; Pinal, N; De Blas, AL
      The Journal of biological chemistry 289 29420-36 2014

      Show Abstract
      25193658 25193658
      Neural circuit interactions between the dorsal raphe nucleus and the lateral hypothalamus: an experimental and computational study.
      Jalewa, J; Joshi, A; McGinnity, TM; Prasad, G; Wong-Lin, K; Hölscher, C
      PloS one 9 e88003 2014

      Show Abstract
      24516577 24516577
      Age- and light-dependent development of localised retinal atrophy in CCL2(-/-)CX3CR1(GFP/GFP) mice.
      Chen, M; Hombrebueno, JR; Luo, C; Penalva, R; Zhao, J; Colhoun, L; Pandi, SP; Forrester, JV; Xu, H
      PloS one 8 e61381 2013

      Show Abstract
      23637822 23637822
      Survey on amacrine cells coupling to retrograde-identified ganglion cells in the mouse retina.
      Pang, JJ; Paul, DL; Wu, SM
      Investigative ophthalmology & visual science 54 5151-62 2013

      Show Abstract
      23821205 23821205
      Anatomical characterization of a rabbit cerebellar eyeblink premotor pathway using pseudorabies and identification of a local modulatory network in anterior interpositus.
      Gonzalez-Joekes, J; Schreurs, BG
      The Journal of neuroscience : the official journal of the Society for Neuroscience 32 12472-87 2012

      Show Abstract
      Immunohistochemistry22956838 22956838
      Rod and cone pathway signalling is altered in the P2X7 receptor knock out mouse.
      Vessey, KA; Fletcher, EL
      PloS one 7 e29990 2012

      Show Abstract
      22253851 22253851

      Data Sheet