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MABN696 | Anti-G6PD Antibody, clone 2H7

100 µL  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, IHC, FC M Ascites Monoclonal Antibody
      Catalogue NumberMABN696
      DescriptionAnti-G6PD Antibody, clone 2H7
      Alternate Names
      • Glucose-6-phosphate dehydrogenase
      • G6PD
      Background InformationGlucose-6-Phosphate Dehydrogenase (G6PD) is the rate-limiting enzyme in the pentose phosphate pathway that catalyzes the oxidation of catalyzes the oxidation of glucose-6-phosphate (G6P) to form pentose sugars for subequent biosynthetic pathways. Glucose-6-Phosphate Dehydrogenase also produces nicotinamide adenine dinucleotide phosphate (NADPH), an important electron donor that plays a protective role against oxidative stress, especially in red blood cells. Deficiencies in glucose-6-phosphate dehydrogenase may disrupt cell growth, cell signaling, and embryonic development. It is also fairly well established that mutations in the glucose-6-phosphate dehydrogenase result in a highly varied condition known as chronic non-spherocytic hemolytic anemia.
      Product Information
      • HeLa, MCF-7, Jurkat and K562 cell lysates
      PresentationMouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
      ApplicationDetect Glucose-6-Phosphate Dehydrogenase using this mouse monoclonal antibody, Anti-G6PD Antibody, clone 2H7 validated for use in western blotting, IHC & Flow Cytometry.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Flow Cytometry
      Application NotesImmunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected G6PD in human breast cancer and kidney cancer tissues.

      ELISA (Direct) Analysis: A 1:10,000 dilution from a representative lot detected G6PD with recombinant G6PD protein antigen.

      Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected G6PD in non serum starved Jurkat cells.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified recombinant fragment of human G6PD expressed in E. Coli.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • G6PD1
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~59 kDa observed. Uncharacterized bands may appear in some lysate(s).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa, MCF-7, Jurkat and K562 cell lysates.

      Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected G6PD in HeLa, MCF-7, Jurkat and K562 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Primary Antibodies