A part of MilliporeSigma

05-1482 | Anti-GβL/mLST8 Antibody, clone 3E1.2

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M WB, ICC M Purified Monoclonal Antibody
      Catalogue Number05-1482
      DescriptionAnti-GβL/mLST8 Antibody, clone 3E1.2
      Alternate Names
      • G protein beta subunit-like
      • Protein GbetaL
      • TORC subunit LST8
      • Target of rapamycin complex subunit LST8
      Background InformationGβL (G protein beta protein subunit-like), also known as mLST8, is a member of the mammalian Target of Rapamycin (mTOR) complex. The complex composed of mTOR, regulatory associated protein of mTOR (Raptor) and mammalian LST8/G-protein β-subunit like protein (mLST8/GβL) is responsible for regulating cell growth and nutrient and growth factor-mediated mTOR signaling.
      Product Information
      • HEK293 cell lysate
      PresentationPurified mouse ascites in buffer containing
      0.1 M Tris-glycine, pH 7.4, 150 mM NaCl and 0.05% sodium azide.
      ApplicationThis Anti-GβL/mLST8 Antibody, clone 3E1.2 is validated for use in WB, IC for the detection of GβL/mLST8.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis:
      Biological Information
      ImmunogenGST-tagged recombinant human GβL/mLST8.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes GβL/mLST8.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteProven to react with human and mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Gable
      • LST8
      • MGC111011
      • OTTHUMP00000159046
      • POP3
      • WAT1
      • mLST8
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Unessential component of the TORC1 complex and essential component of the TORC2 complex. TORC1 controls cell growth in response to environmental signals, and is inactivated by nutrient limitation and environmental stress. Within TORC1, LST8 interacts directly with FRAP1 and enhances its kinase activity. In nutrient-poor conditions, stabilizes the FRAP1-RAPTOR interaction and favors RAPTOR-mediated inhibition of FRAP1 activity. TORC2 controls polarity of the actin cytoskeleton via the RAC1 pathway. TORC2 mediates phosphorylation of Akt/PKB on 'Ser-473' and phosphorylation of PKCalpha on 'Ser-657'.

      SUBUNIT STRUCTURE: Interacts with FRAP1, RAPTOR and RHEB. Part of the target of rapamycin complex 1 (TORC1) which contains LST8, FRAP1, RAPTOR and AKT1S1. TORC1 binds to and is inhibited by FKBP12-rapamycin. Part of the target of rapamycin complex 2 (TORC2) which contains FRAP1, LST8, PROTOR1, RICTOR and MAPKAP1. Contrary to TORC1, TORC2 does not bind to and is not sensitive to FKBP12-rapamycin.

      SUBCELLULAR LOCATION: Cytoplasm By similarity.

      TISSUE SPECIFICITY: Broadly expressed, with highest levels in skeletal muscle, heart and kidney. Ref.6

      SEQUENCE SIMILARITIES: Belongs to the WD repeat LST8 family.

      Contains 7 WD repeats.

      Molecular Weight36 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HEK293 cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected GβL/mLST8 in HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C for 1 year from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information