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MAB88916-C | Anti-Fibronectin (Cell Attachment Fragment) Antibody, clone 3E3, Ascites & Azide Free

MAB88916-C
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, IHC, ELISA, ICC, Inhibition M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB88916-C
      DescriptionAnti-Fibronectin (Cell Attachment Fragment) Antibody, clone 3E3, Ascites & Azide Free
      Alternate Names
      • Fibronectin
      • FN
      • CIG
      • Cold-insoluble globulin
      Background InformationFibronectin (FN), or in earlier literature also known as Cold-insoluble globulin (CIG), and is encoded by the gene named FN1/FN is an extracellular matrix protein. Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Fibronectin also plays an important role in bone development and bone structure formation. Additionally, when fibronectin binds the protein anastellin it forms a multiprotein complex termed superfibronectin that has been shown to inhibit tumor growth, angiogenesis and metastasis. Fibronectin protein can be found extracellularly as well as intracellularly. There are multiple isoforms produced by alternative splicing. Plasma fibronectin (a soluble dimeric form) is secreted by hepatocytes into the blood. Cellular fibronectin (dimeric or cross-linked multimeric forms), are made by fibroblasts, epithelial and other cell types, and is deposited as fibrils in the extracellular matrix.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without preservatives.
      Applications
      ApplicationThis Anti-Fibronectin (Cell Attachment Fragment) Antibody, clone 3E3, Ascites & Azide Free is validated for use in western blotting, IHC, ELISA, ICC & inhibition for the detection of Fibronectin.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Enzyme Immunoassay (ELISA)
      • Immunocytochemistry
      • Inhibition
      Application NotesWestern Blotting Analysis: 0.5 µg/mL of this antibody detected Fibronectin (Cell Attachment Fragment) in 10 µg of U-251 cell lysate.

      Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Fibronectin (Cell Attachment Fragment) in human placenta and liver tissues.

      ELISA Analysis: A representative lot detected the conformation of Fibronectin adsorbed onto self-assembled monolayers (SAMs) of alkanethiols over a range of Fibroectin surface densities (Kesolowsky, B. G., et al. (2003). J Biomed Mater Res A. 66(2):247-259.).

      Immuncytochemistry Analysis: A representative lot detected Fibronectin (Cell Attachment Fragment) in Extracellular matrix deposited by HUVECs (Orecchia, A., et al. (2003). J Cell Sci. 116(Pt 17):3479-3489.).

      Inhibition Analysis: A representative lot blocked cell-adhesive epitopes of Fibronectin (Cell Attachment Fragment) (Miller, D. C., et al. (2005). J Biomed Mater Res A. 73(4):476-84.).

      Inhibition Analysis: A representative lot blocked RGD-dependent cell adhesion (Tsang, T. M., et al. (2012). J Biol Chem. 287(20):16759-16767.).

      Inhibition Analysis: A representative lot inhibited the cell-adhesive epitopes of Fibronectin (Miller, D. C., et al. (2005). J Biomed Mater Res. 73(4):476-484.)
      Biological Information
      ImmunogenPurified human Fibronectin, which contains the Cell Attachment Fragment, from amnion liquid.
      EpitopeThis antibody recognizes the cell attachment fragment of Fibronectin.
      Clone3E3
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • FN1
      • FN
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~260 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Fibronectin (Cell Attachment Fragment) in 10 µg of HepG2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Fibronectin (Cell Attachment -2811638 2811638
      Anti-Fibronectin (Cell Attachment Fragment), clone 3E3, Ascites & Azide -2683289 2683289
      Anti-Fibronectin (Cell Attachment Fragment), clone 3E3, Ascites & Azide Free - Q2399123 Q2399123

      References

      Reference overviewPub Med ID
      Arg-Gly-Asp: a versatile cell recognition signal.
      Ruoslahti, E and Pierschbacher, M D
      Cell, 44: 517-8 (1986) 1986

      2418980 2418980
      Detachment of cells from culture substrate by soluble fibronectin peptides.
      Hayman, E G, et al.
      J. Cell Biol., 100: 1948-54 (1985) 1985

      Show Abstract
      2581980 2581980
      Cell attachment activity of fibronectin can be duplicated by small synthetic fragments of the molecule.
      Pierschbacher, M D and Ruoslahti, E
      Nature, 309: 30-3 1984

      Show Abstract
      6325925 6325925
      Synthetic peptide with cell attachment activity of fibronectin.
      Pierschbacher, M, et al.
      Proc. Natl. Acad. Sci. U.S.A., 80: 1224-7 (1983) 1983

      Show Abstract
      6572380 6572380
      The cell attachment domain of fibronectin. Determination of the primary structure.
      Pierschbacher, M D, et al.
      J. Biol. Chem., 257: 9593-7 (1982) 1982

      Show Abstract
      7050098 7050098

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies