MAB3582 Anti-Fascin Antibody, clone 55K2

MAB3582
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Ma, Ech, Gr, Su WB, ICC, IH(P) M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB3582
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Fascin Antibody, clone 55K2
      References
      Product Information
      FormatPurified
      Control
      • POSITIVE CONTROL:

        Hela cells, neuronal tissues, antigen presenting dendritic cells.
      PresentationLiquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide.
      Applications
      ApplicationThis Anti-Fascin Antibody, clone 55K2 is validated for use in WB, IC, IH(P) for the detection of Fascin.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      Applications Not Recommended
      • Immunoprecipitation
      Application NotesWestern blot. Recognizes a protein of 55 kDa. Suggested working dilution is 1:100-1:1,000. Suggested blocking buffer is 3% BSA in PBS or 3% non fat milk in PBS.

      Immunohistochemistry on formalin fixed, paraffin embedded tissue sections. Suggested working dilution is 1:100-1:1,000.

      Immunocytochemistry on cold methanol fixed cultured cells. Suggested working dilution is 1:100-1:1,000.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenHuman fascin
      Clone55K2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityFascin. The antibody is strongly positive when antigen-presenting dendritic cells are matured. MAB3582 is a sensitive marker for Reed-Sternberg Cells of Hodgkin's Disease. The protein is an actin bundling protein specifically localized in filopodia and growth cones.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mammals
      • Echinoderms
      • Gerbil
      • Sea Urchin
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • FSCN1
      • FAN1
      • HSN
      • FLJ38511
      • SNL
      • Fascin
      • p55
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16658 # Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Probably involved in the assembly of actin filament bundles present in microspikes, membrane ruffles, and stress fibers.
      SIZE: 493 amino acids; 54530 Da
      SUBUNIT: Associates with beta-catenin.
      TISSUE SPECIFICITY: Ubiquitous.
      PTM: Phosphorylation on Ser-39 inhibits the actin-binding ability of fascin.
      DISEASE: SwissProt: Q16658 # Marks and mediates breast cancer metastasis to the lungs. FSCN1 is not functionally validated but achieves the highest statistical significance (P less than 0.000001). Those subjects expressing the lung metastasis signature have a significantly poorer lung metastasis-free survival, but not bone metastasis-free survival, compared to subjects without the signature.
      SIMILARITY: SwissProt: Q16658 ## Belongs to the fascin family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots up to 6 months after date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-FASCIN3068481
      MOUSE ANTI-FASCIN MONOCLONAL ANTIBODY - 23910752391075
      MOUSE ANTI-FASCIN MONOCLONAL ANTIBODY - 21530202153020
      MOUSE ANTI-FASCIN - 20909352090935
      MOUSE ANTI-FASCIN -25178002517800
      MOUSE ANTI-FASCIN -27393212739321
      MOUSE ANTI-FASCIN MONOCLONAL ANTIBODY2942446
      MOUSE ANTI-FASCIN MONOCLONAL ANTIBODY - 21095302109530
      MOUSE ANTI-FASCIN MONOCLONAL ANTIBODY - 22972462297246
      MOUSE ANTI-FASCIN_28369382836938

      References

      Reference overviewPub Med ID
      Protein expression dynamics during postnatal mouse brain development.
      Laeremans, A; Van de Plas, B; Clerens, S; Van den Bergh, G; Arckens, L; Hu, TT
      Journal of experimental neuroscience  7  61-74  2013

      Show Abstract
      25157209 25157209
      The prognostic impact of TGF-β1, fascin, NF-κB and PKC-ζ expression in soft tissue sarcomas.
      Valkov, A; Sorbye, SW; Kilvaer, TK; Donnem, T; Smeland, E; Bremnes, RM; Busund, LT
      PloS one  6  e17507  2011

      Show Abstract Full Text Article
      21390241 21390241
      The prognostic impact of NF-kappaB p105, vimentin, E-cadherin and Par6 expression in epithelial and stromal compartment in non-small-cell lung cancer.
      Al-Saad, S; Al-Shibli, K; Donnem, T; Persson, M; Bremnes, RM; Busund, LT
      British journal of cancer  99  1476-83  2008

      Show Abstract
      18854838 18854838
      Biologic significance of fascin expression in clear cell renal cell carcinoma: systematic analysis of primary and metastatic tumor tissues using a tissue microarray technique.
      Richard Zigeuner, Nikolaus Droschl, Volkmar Tauber, Peter Rehak, Cord Langner
      Urology  68  518-22  2006

      Show Abstract
      16979727 16979727
      Arp2/3 is a negative regulator of growth cone translocation.
      Strasser, GA; Rahim, NA; VanderWaal, KE; Gertler, FB; Lanier, LM
      Neuron  43  81-94  2004

      Show Abstract
      15233919 15233919
      Role of the actin bundling protein fascin in growth cone morphogenesis: localization in filopodia and lamellipodia.
      Cohan, C S, et al.
      Cell Motil. Cytoskeleton, 48: 109-20 (2001)  2001

      Show Abstract
      11169763 11169763
      Fascin, an actin-bundling protein, induces membrane protrusions, and increases cell motility of epithelial cells
      Yamashiro, S., Yamakita, Y., Ono, S., and Matsumura, F.
      Mol. Biol. Cell, 9:993-1006 (1998)  1998

      9571235 9571235
      Fascin: A sensitive new marker for Reed-Sternberg cells of Hodgkin's Disease - Evidence for a dendritic or B cell derivation?
      Pinkus, G. S., Pinkus, J. L., Langhoff, E., Matsumura, F., Yamashiro, S., Mosialos, G., and Said, J. W.
      American J. Pathology, 150:43-562 (1997)  1997

      9033270 9033270
      Epstein-Barr virus infection induces expression in B lymphocytes of a novel gene encoding an evolutionary conserved 55-kD actin-bundling protein
      Mosialos, G., Yamashiro, S., Baughman, R.W., Matsudaira, P., Vara, L., Matsumura, F., Kieff, E., and Birkenbach, M.
      J. Virology, 68:7320-7328 (1994)  1994

      7933116 7933116
      Intracellular localization of 55kDa actin-bundling protein in cultured cells: spatial relationships with actin, alpha-actinin, tropomyosin, and fimbrin
      Yamashiro-Matsumura, S. and Matsumura, F.
      J. Cell Biol., 103:631-640 (1985)  1985

      3525578 3525578

      Data Sheet

      Title
      Anti-Fascin, clone 55K2 - Data Sheet