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PC69 | Anti-Fas (Ab-1) (321-335) Rabbit pAb

PC69
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, Op, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      PC69-100UG
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      Stocked 
      Discontinued
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      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service

          Glass bottle 100 μg
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          Description
          OverviewRecognizes the ~44-46 kDa Fas protein in HL60 cells and color tissue.
          The immunogen, Fas Peptide (Cat. No. PP62), is available for competition studies.
          Catalogue NumberPC69
          Brand Family Calbiochem®
          SynonymsAnti-APO-1, Anti-CD95
          References
          ReferencesCheng, J., et al. 1995. J. Immunol. 154, 1239.
          Stanger, B.Z., et al. 1995. Cell 81, 513.
          Grell, M., et al. 1994. Eur. J. Immunol. 24, 2563.
          Owen-Schaub, L.B., et al. 1994. Cancer Res. 54, 1580.
          Itoh, N. and Nagata, S. 1993. J. Biol. Chem. 268, 10932.
          Oehm, A., et al. 1992. J. Biol. Chem. 267, 10709.
          Watanabe-Fukunaga, R., et al. 1992. J. Immunol. 148, 1274.
          Itoh, N., et al. 1991. Cell 66, 233.
          Product Information
          FormLiquid
          FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
          Positive controlHL-60 or colon tissue
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Frozen Sections
          Immunoblotting (Western Blotting)
          Paraffin Sections
          Application NotesFrozen Sections (2.5 µg/ml)
          Immunoblotting (2.5 µg/ml)
          Paraffin Sections (2.5 µg/ml, pressure cooker pre-treatment required)
          Application CommentsWill detect Fas using frozen sections but not immunoblots of mouse and rat samples. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic peptide (DSENSNFRNEIQSLV) corresponding to amino acids 321-335 of human Fas
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          • Mouse
          • Opossum
          • Rat
          Antibody TypePolyclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          PC69

          References

          Reference overview
          Cheng, J., et al. 1995. J. Immunol. 154, 1239.
          Stanger, B.Z., et al. 1995. Cell 81, 513.
          Grell, M., et al. 1994. Eur. J. Immunol. 24, 2563.
          Owen-Schaub, L.B., et al. 1994. Cancer Res. 54, 1580.
          Itoh, N. and Nagata, S. 1993. J. Biol. Chem. 268, 10932.
          Oehm, A., et al. 1992. J. Biol. Chem. 267, 10709.
          Watanabe-Fukunaga, R., et al. 1992. J. Immunol. 148, 1274.
          Itoh, N., et al. 1991. Cell 66, 233.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision26-September-2007 RFH
          SynonymsAnti-APO-1, Anti-CD95
          ApplicationFrozen Sections (2.5 µg/ml)
          Immunoblotting (2.5 µg/ml)
          Paraffin Sections (2.5 µg/ml, pressure cooker pre-treatment required)
          DescriptionPurified rabbit polyclonal antibody. Recognizes the ~44-46 kDa Fas protein.
          BackgroundFas/APO-1 was originally described as a cell surface antigen on activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. The gene encoding the human fas antigen consists of 9 exons, spans more than 26 kb of DNA and shares 58.5% homology with the mouse homolog. The protein contains a single transmembrane domain which is rich in cysteine residues and an inhibitory as well as a signal transducing or death domain in the cytoplasmic domain. Ligation or crosslinking of the extracellular domain by fas ligand or by antibody elicits a characteristic apoptotic response in susceptible cells including lymphoid cells and tumors as well as non-hematopoietic tumors. Upon activation, fas associates with a number of other proteins to mediate the response. Fas is a member of the TNF receptor family and shares some sequence homology with the TNF receptor but does not appear to physically interact with TNF-R itself.
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic peptide (DSENSNFRNEIQSLV) corresponding to amino acids 321-335 of human Fas
          IsotypeIgG
          Specieshuman, mouse, opossum, rat
          Positive controlHL-60 or colon tissue
          FormLiquid
          FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsWill detect Fas using frozen sections but not immunoblots of mouse and rat samples. Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          ReferencesCheng, J., et al. 1995. J. Immunol. 154, 1239.
          Stanger, B.Z., et al. 1995. Cell 81, 513.
          Grell, M., et al. 1994. Eur. J. Immunol. 24, 2563.
          Owen-Schaub, L.B., et al. 1994. Cancer Res. 54, 1580.
          Itoh, N. and Nagata, S. 1993. J. Biol. Chem. 268, 10932.
          Oehm, A., et al. 1992. J. Biol. Chem. 267, 10709.
          Watanabe-Fukunaga, R., et al. 1992. J. Immunol. 148, 1274.
          Itoh, N., et al. 1991. Cell 66, 233.