341286 Anti-Farnesyl Rabbit pAb

341286
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      A Broad Range Of Species Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      341286-100UG
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      Stocked 
      Discontinued
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          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes farnesyl. Also recognizes KLH.
          Catalogue Number341286
          Brand Family Calbiochem®
          Application Data
          Detection of farnesyl by immunofluorescence. Sample: Plasmids encoding isoprenylated protein. Primary antibody: Anti-Farnesyl Rabbit pAb (Cat. No. 341286) (assay dependent on Primary Antibody). Detection: fluorescence.
          References
          ReferencesBaron, R., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 11626.
          Lin, H.P., et al. 1999. J. Gen. Virol. 80, 91.
          Aspbury, R.A., et al. 1998. Biochim Biophys Acta 1392, 265.
          Gromov, P.S., et al. 1996. Electrophoresis 17, 1728.
          Zhang, F.L. & Casey, P.J. 1996. Annu. Rev. Biochem. 65, 241.
          Product Information
          FormLiquid
          FormulationIn PBS.
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunofluorescence
          Application NotesELISA (1:1000)
          Immunofluorescence (1:50)
          Immunoblotting (not recommended; see comments)
          Application CommentsRecognizes proteins that have been isoprenylated by farnesyltransferase of geranylgeranyltransferase at the C-A-A-X motif. Also recognizes KLH. Not suitable for immunoblotting due to the hydrophobicity of the isoprenylated motif. Variables associated with assay conditions will dictate the optimal working dilution.
          Biological Information
          Immunogenfarnesyl cysteine conjugated to KLH
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • A Broad Range Of Species
          Antibody TypePolyclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          341286

          References

          Reference overview
          Baron, R., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 11626.
          Lin, H.P., et al. 1999. J. Gen. Virol. 80, 91.
          Aspbury, R.A., et al. 1998. Biochim Biophys Acta 1392, 265.
          Gromov, P.S., et al. 1996. Electrophoresis 17, 1728.
          Zhang, F.L. & Casey, P.J. 1996. Annu. Rev. Biochem. 65, 241.

          Brochure

          Title
          Antibody Sourcebook!" Second Edition
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision04-October-2007 RFH
          ApplicationELISA (1:1000)
          Immunofluorescence (1:50)
          Immunoblotting (not recommended; see comments)
          Application Data
          Detection of farnesyl by immunofluorescence. Sample: Plasmids encoding isoprenylated protein. Primary antibody: Anti-Farnesyl Rabbit pAb (Cat. No. 341286) (assay dependent on Primary Antibody). Detection: fluorescence.
          DescriptionPurified rabbit polyclonal antibody. Recognizes farnesyl.
          BackgroundPrenylation of proteins is a post-translational modification that is necessary for membrane association of proteins as well as protein-protein interactions. The nature of the linked isoprenyl group can influence the protein interactions, such as the interaction between G proteins and receptors. Approximately 0.5% of cellular proteins are affected by protein isoprenylation. Farnesyltransferase and geranylgeranyltransferase are the two enzymes involved in catalyzing the attachment of two prenyl groups to the sulfhydryl group of the C-terminal cysteine groups. These enzymes transfer a 15 carbon farnesyl or a 20 carbon geranygeranyl, respectively, from a prenyl-pyrophosphate to the protein. Proteins which are prenylated by these enzymes have a distinct motif at the C-terminal of the protein, C-A1-A2-X (C= Cysteine, A1 & A2 = aliphatic amino acids). Some of the proteins that contain the C-A-A-X motif are members of the Ras small G protein family, the nuclear lamins, and the γ-subunit of trimeric G proteins.
          HostRabbit
          Immunogenfarnesyl cysteine conjugated to KLH
          IsotypeIgG
          Speciesa broad range of species
          FormLiquid
          FormulationIn PBS.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsRecognizes proteins that have been isoprenylated by farnesyltransferase of geranylgeranyltransferase at the C-A-A-X motif. Also recognizes KLH. Not suitable for immunoblotting due to the hydrophobicity of the isoprenylated motif. Variables associated with assay conditions will dictate the optimal working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesBaron, R., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 11626.
          Lin, H.P., et al. 1999. J. Gen. Virol. 80, 91.
          Aspbury, R.A., et al. 1998. Biochim Biophys Acta 1392, 265.
          Gromov, P.S., et al. 1996. Electrophoresis 17, 1728.
          Zhang, F.L. & Casey, P.J. 1996. Annu. Rev. Biochem. 65, 241.