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ST1630 | Anti-FGF8 Mouse mAb (2A10)

ST1630
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, R M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      ST1630-100UG
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      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
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      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service

          100 μg
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          Description
          OverviewRecognizes the ~30-35 kDa FGF8 protein in PC-12, Jurkat, HepG2, NIH3T3, and Raw 264.7 cells.
          Catalogue NumberST1630
          Brand Family Calbiochem®
          SynonymsAnti-Fibroblast Growth Factor 8
          Application Data
          Detection of rat FGF8 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-FGF8 Mouse mAb (2A10) (Cat. No. ST1630) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human FGF8 by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-FGF8 Mouse mAb (2A10) (Cat. No. ST1630) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of mouse FGF8 by immunoblotting. Sample: Whole cell lysate from Raw 264.7 cells. Primary antibody: Anti-FGF8 Mouse mAb (2A10) (Cat. No. ST1630) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human FGF8 by immunoblotting. Sample: Whole cell lysate from Jurkat cells. Primary antibody: Anti-FGF8 Mouse mAb (2A10) (Cat. No. ST1630) (1-5 µg/ml). Detection: chemiluminescence.
          References
          Product Information
          FormLiquid
          FormulationIn PBS, pH 7.2.
          Positive controlPC-12 cells, Jurkat cells, HepG2 cells, NIH3T3 cells, Raw 264.7 cells
          PreservativeNone
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Application NotesELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          ImmunogenFull-length, recombinant, human FGF8 (aa 65-133), expressed as a GST fusion protein
          Clone2A10
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Regulatory Review
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision09-November-2010 RFH
          SynonymsAnti-Fibroblast Growth Factor 8
          ApplicationELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Application Data
          Detection of rat FGF8 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-FGF8 Mouse mAb (2A10) (Cat. No. ST1630) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human FGF8 by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-FGF8 Mouse mAb (2A10) (Cat. No. ST1630) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of mouse FGF8 by immunoblotting. Sample: Whole cell lysate from Raw 264.7 cells. Primary antibody: Anti-FGF8 Mouse mAb (2A10) (Cat. No. ST1630) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human FGF8 by immunoblotting. Sample: Whole cell lysate from Jurkat cells. Primary antibody: Anti-FGF8 Mouse mAb (2A10) (Cat. No. ST1630) (1-5 µg/ml). Detection: chemiluminescence.
          DescriptionPurified mouse monoclonal antibody. Recognizes the ~30-35 kDa FGF8 protein.
          BackgroundThe protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is known to be a factor that supports androgen and anchorage independent growth of mammary tumor cells. Overexpression of this gene has been shown to increase tumor growth and angiogensis. The adult expression of this gene is restricted to testes and ovaries. Temporal and spatial pattern of this gene expression suggests its function as an embryonic epithelial factor. Studies of the mouse and chick homologs revealed roles in midbrain and limb development, organogenesis, embryo gastrulation and left-right axis determination. The alternative splicing of this gene results in four transcript variants.
          HostMouse
          ImmunogenFull-length, recombinant, human FGF8 (aa 65-133), expressed as a GST fusion protein
          Clone2A10
          IsotypeIgG2a
          Specieshuman, mouse, rat
          Positive controlPC-12 cells, Jurkat cells, HepG2 cells, NIH3T3 cells, Raw 264.7 cells
          FormLiquid
          FormulationIn PBS, pH 7.2.
          PreservativeNone
          CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Regulatory Review