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16-173 | Anti-FAK Antibody, clone 4.47, agarose conjugate

16-173
200 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H IP M Gel Immobilized Monoclonal Antibody
      Description
      Catalogue Number16-173
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-FAK Antibody, clone 4.47, agarose conjugate
      OverviewAnti-FAK monoclonal antibody (05-537), covalently coupled to Protein G agarose by dimethyl-pimelimidate
      References
      Product Information
      FormatGel Immobilized
      PresentationPBS containing 0.05% sodium azide
      Applications
      ApplicationThis Anti-FAK Antibody, clone 4.47, agarose conjugate is validated for use in IP for the detection of FAK.
      Key Applications
      • Immunoprecipitation
      Application NotesSuitable for immunoprecipitation and affinity purification.
      Biological Information
      Cloneclone 4.47
      HostMouse
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined.
      Gene Symbol
      • PTK2
      • pp125FAK
      • FAK
      • FADK
      • FAK1
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q05397 # Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
      SIZE: 1052 amino acids; 119233 Da
      SUBUNIT: Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with SHB (By similarity). Interacts with TGFB1I1.
      SUBCELLULAR LOCATION: Cell junction, focal adhesion; Cytoplasmic side. Note=Constituent of focal adhesions.
      TISSUE SPECIFICITY: Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
      DOMAIN: SwissProt: Q05397 The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL (By similarity). & The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
      PTM: Phosphorylated on 6 tyrosine residues upon activation.
      SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. & Contains 1 FERM domain. & Contains 1 protein kinase domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoprecipitating FAK from a mouse 3T3/A31 RIPA cell lysate, as confirmed by subsequent immunoblot analysis using anti-FAK (05-537)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at 4°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-FAK, clone 4.47, agarose conjugate (mouse monoclonal IgG1) - 2444745 2444745
      Anti-FAK, clone 4.47, agarose conjugate (mouse monoclonal IgG1) - 2208821 2208821
      Anti-FAK, clone 4.47, agarose conjugate (mouse monoclonal IgG1) - DAM1428212 DAM1428212
      Anti-FAK, clone 4.47, agarose conjugate - 19715 19715
      Anti-FAK, clone 4.47, agarose conjugate - 21097 21097
      Anti-FAK, clone 4.47, agarose conjugate - 27737 27737
      Anti-FAK, clone 4.47, agarose conjugate - 28908 28908
      Anti-FAK, clone 4.47, agarose conjugate - DAM1557450 DAM1557450
      Anti-FAK, clone 4.47, agarose conjugate - DAM1713313 DAM1713313
      Anti-FAK, clone 4.47, agarose conjugate - JBC1873458 JBC1873458
      Anti-FAK, clone 4.47, agarose conjugate -2591868 2591868
      Anti-FAK, clone 4.47, agarose conjugate -2679379 2679379
      Anti-FAK, clone 4.47, agarose conjugate, (mouse monoclonal IgG1) 2820299

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      Alternative Format

      Catalog Number Description  
      05-537 Anti-FAK Antibody, clone 4.47 Show Pricing & Availability
      16-233 Anti-FAK Antibody, clone 4.47, Alexa Fluor® 488 conjugate Show Pricing & Availability
      16-234 Anti-FAK Antibody, clone 4.47, Alexa Fluor® 555 conjugate Show Pricing & Availability

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies