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ST1579 | Anti-FADS1 Mouse mAb (2D9)

ST1579
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      ST1579-100UG
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      Stocked 
      Discontinued
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      Available
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          100 μg
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          Description
          OverviewRecognizes the ~50-55 kDa FADS1 protein in HeLa cells and human skeletal muscle tissue.
          Catalogue NumberST1579
          Brand Family Calbiochem®
          SynonymsAnti-Fatty Acid Desaturase 1
          Application Data
          Detection of human FADS1 by immunoblotting. Sample: Whole cell lysates from HeLa NE cells. Primary antibody: Anti-FADS1 Mouse mAb (2D9) (Cat. No. ST1579) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human FADS1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human skeletal muscle. Primary antibody: Anti-FADS1 Mouse mAb (2D9) (Cat. No. ST1579) (3 µg/ml). Detection: colorimetric (DAB).
          References
          Product Information
          FormLiquid
          FormulationIn PBS, pH 7.2.
          Positive controlHeLa cells, Human skeletal muscle tissue
          PreservativeNone
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Paraffin Sections
          Application NotesELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Paraffin Sections (3 µg/ml)
          Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          ImmunogenA recombinant polypeptide corresponding to amino acids 1-100 of human FADS1, expressed as a GST fusion protein
          Clone2D9
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Regulatory Review
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision09-November-2010 RFH
          SynonymsAnti-Fatty Acid Desaturase 1
          ApplicationELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Paraffin Sections (3 µg/ml)
          Application Data
          Detection of human FADS1 by immunoblotting. Sample: Whole cell lysates from HeLa NE cells. Primary antibody: Anti-FADS1 Mouse mAb (2D9) (Cat. No. ST1579) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human FADS1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human skeletal muscle. Primary antibody: Anti-FADS1 Mouse mAb (2D9) (Cat. No. ST1579) (3 µg/ml). Detection: colorimetric (DAB).
          DescriptionPurified mouse monoclonal antibody. Recognizes the ~50-55 kDa FADS1 protein.
          BackgroundFADS1 is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs.
          HostMouse
          ImmunogenA recombinant polypeptide corresponding to amino acids 1-100 of human FADS1, expressed as a GST fusion protein
          Clone2D9
          IsotypeIgG2a
          Specieshuman
          Positive controlHeLa cells, Human skeletal muscle tissue
          FormLiquid
          FormulationIn PBS, pH 7.2.
          PreservativeNone
          CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Regulatory Review