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MABE290 | Anti-Estrogen Receptor β Antibody, clone 9.88

100 µL  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, M, H WB, IP M Purified Monoclonal Antibody
      Catalogue NumberMABE290
      DescriptionAnti-Estrogen Receptor β Antibody, clone 9.88
      Alternate Names
      • Estrogen receptor beta
      • ER-beta
      • Nuclear receptor subfamily 3 group A member 2
      Background InformationEstrogen receptor beta (ERβ; NR3A2 or ESR2) is a nuclear steroid receptor that has been detected in the ovary, testis, thymus, spleen, bone, brain, and kidney. It is a multidomain protein containing A/B, C, D, E, and F domains, and it is activated by estrogen, resulting in homo- or heterodimerization (with ERα). The active ERβ-estrogen complex binds to regulatory regions known as estrogen response elements on target genes; however, ERβ may also regulate transcription without a bound ligand via its activation function (AF-1) region in the A/B domain. The ERβ receptor interacts with a wide variety of proteins including transcriptional corepressors and coactivators that regulate its activity. Its expression is also regulated by hypermethylation of CpG islands in the ERβ promoter. Previous studies have reported that ERβ has anti-inflammatory and anti-proliferative effects, and may therefore be an important therapeutic target for cancers, especially those occurring in the reproductive organs.
      Product Information
      • PC-12 cell lysate
      PresentationPurified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
      ApplicationUse Anti-Estrogen Receptor β Antibody, clone 9.88 (Mouse Monoclonal Antibody) validated in WB, IP to detect Estrogen Receptor β also known as Estrogen receptor beta.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Estrogen Receptor β in HUVEC cells (Simoncini, T., et al. (2006). Mol Endocrinol. 20(8):1756-1771.).
      Biological Information
      ImmunogenRecombinant protein corresponding to human Estrogen Receptor β.
      Species Reactivity
      • Rat
      • Mouse
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized.
      Gene Symbol
      • ESR2
      • ESTRB
      • NR3A2
      Purification MethodPurified by Ion-Exchange Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.

      SUBUNIT STRUCTURE: Binds DNA as a homodimer. Can form a heterodimer with ESR1. Interacts with NCOA3, NCOA5 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with PELP1 and UBE1C. Isoform beta-2/cx preferentially forms a hetereodimer with ESR1 rather than ESR2 and inhibits DNA-binding by ESR1. Interacts with AKAP13. Interacts with DNTTIP2. Interacts with isoform 4 of TXNRD1. Interacts with CCDC62 in the presence of estradiol/E2; this interaction seems to enhance the transcription of target genes, including cyclin-D1/CCND1 AP-1 promoter. Interacts with DYX1C1. Interacts with PRMT2. Interacts with PRMT2.


      TISSUE SPECIFICITY: Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.

      DOMAIN: Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

      SEQUENCE SIMILARITIES: Belongs to the nuclear hormone receptor family. NR3 subfamily. Contains 1 nuclear receptor DNA-binding domain.

      SEQUENCE CAUTION: The sequence CAA67555.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
      Molecular Weight~55 kDa observed. Uniprot describes eight different isoforms ranging from ~36 kDa to ~59 kDa.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in PC-12 cell lysate.

      Western Blot Analysis: A 1:1,000 dilution of this antibody detected Estrogen Receptor β in PC-12 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Estrogen Receptor , clone 9.88 - Q2009754 Q2009754
      Anti-Estrogen Receptor β, clone 9.88 - 2433592 2433592
      Anti-Estrogen Receptor β, clone 9.88 -2509367 2509367
      Anti-Estrogen Receptor β, clone 9.88 -2756672 2756672


      Reference overviewPub Med ID
      Estrogen receptor alpha interacts with Galpha13 to drive actin remodeling and endothelial cell migration via the RhoA/Rho kinase/moesin pathway.
      Simoncini, Tommaso, et al.
      Mol. Endocrinol., 20: 1756-71 (2006) 2006

      Show Abstract
      16601072 16601072


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      Life Science Research > Antibodies and Assays > Primary Antibodies