A part of MilliporeSigma

04-820 | Anti-Estrogen Receptor α Antibody, clone 60C, rabbit monoclonal

100 µL  
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      Replacement Information
      Replacement Information04-820 is a recommended replacement for 05-820

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, B, Po WB, IP Rb Culture Supernatant Monoclonal Antibody
      Catalogue Number04-820
      DescriptionAnti-Estrogen Receptor α Antibody, clone 60C, rabbit monoclonal
      Alternate Names
      • Estradiol receptor
      • Nuclear receptor subfamily 3 group A member 1
      • estrogen receptor 1
      • estrogen receptor alpha
      • oestrogen receptor
      • steroid hormone receptor
      Background InformationThe Estrogen Receptors (ER) belong to the nuclear receptor family that contains a ligand-binding domain that maintains the protein in an inactive conformation unless bound to the hormone. Two ERs are known, ERα, classically known as ER, and ERβ, described long after ERα. Activated Estrogen Receptor is a DNA-binding protein capable of inducing many changes in gene expression. The Estrogen Receptor is believed to play an important role in the growth and development of a subset of hormone dependent human breast cancers. ERβ is expressed in prostate, ovary, and other reproductive organs. Although its steroid specificity is very similar to that of the ERα, the molecular mechanisms regulating the transcriptional activity of ERβ may be distinct from those regulating ERα.
      Product Information
      FormatCulture Supernatant
      PresentationCultured supernantant with 0.05% sodium azide.
      ApplicationAnti-Estrogen Receptor α Antibody, clone 60C is an antibody against Estrogen Receptor α for use in WB & IP.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation:
      4-10 μL of a previous lot of this clone immunoprecipitated Estrogen Receptor α from 1 mg of MCF-7 RIPA lysate.

      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 13-32 (C-ALLHQIQGNELEPLNRPQLK) of human Estrogen Receptor α. The immunizing sequence is identical in rat and mouse and has 19/20 identical amino acids in bovine and porcine.
      SpecificityRecognizes Estrogen Receptor α. No cross-reactivity with Estrogen Receptor β.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Bovine
      • Pig
      Species Reactivity NoteHuman. Predicted to cross-react with rat, mouse, bovine and porcine based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummarySummary: This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for
      hormone binding, DNA binding, and activation of transcription. The
      protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast
      cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters. [provided by RefSeq].
      Gene Symbol
      • ER
      • ER-alpha
      • ESR
      • ESRA
      • Era
      • NR3A1
      • DKFZp686N23123
      • OTTHUMP00000017718
      • OTTHUMP00000017719
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P03372 # Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.
      SIZE: 595 amino acids; 66216 Da
      SUBUNIT: Binds DNA as a homodimer. Can form a heterodimer with ESR2. Interacts with NCOA3, NCOA5 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with NCOA7 in a ligand-inducible manner. Interacts with PHB2, PELP1 and UBE1C. Interacts with AKAP13. Interacts with CUEDC2. Interacts with JARID1A. Interacts with SMARD1. Interacts with SLC30A9 (By similarity). Interacts with HEXIM1 and MAP1S. Interacts with PBXIP1.
      DOMAIN: SwissProt: P03372 Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain.
      PTM: Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
      SIMILARITY: Belongs to the nuclear hormone receptor family. NR3 subfamily. & Contains 1 nuclear receptor DNA-binding domain.
      MISCELLANEOUS: In the absence of ligand, steroid hormone receptors are thought to be weakly associated with nuclear components; hormone binding greatly increases receptor affinity. The hormone- receptor complex appears to recognize discrete DNA sequences upstream of transcriptional start sites.
      Molecular Weight66 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot analysis.

      Western Blot Analysis:
      This lot detected ERα at a 1:2,000 dilution in MCF-7 lysates via SDS-PAGE and transferred to PVDF (Immobilon-P).

      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Estrogen Receptor #945;, clone 60C - 2135145 2135145
      Anti-Estrogen Receptor a, clone 60C - 2010682 2010682
      Anti-Estrogen Receptor a, clone 60C - 2034725 2034725
      Anti-Estrogen Receptor a, clone 60C - 2271228 2271228
      Anti-Estrogen Receptor a, clone 60C - JBC1519231 JBC1519231
      Anti-Estrogen Receptor a, clone 60C - NG1650718 NG1650718
      Anti-Estrogen Receptor a, clone 60C - NG1693873 NG1693873
      Anti-Estrogen Receptor a, clone 60C - NG1740967 NG1740967
      Anti-Estrogen Receptor a, clone 60C - NG1804937 NG1804937
      Anti-Estrogen Receptor a, clone 60C - NG1853744 NG1853744
      Anti-Estrogen Receptor α, clone 60C - 2388922 2388922
      Anti-Estrogen Receptor α, clone 60C - 2433535 2433535
      Anti-Estrogen Receptor α, clone 60C - 2828587 2828587
      Anti-Estrogen Receptor α, clone 60C -2504862 2504862
      Anti-Estrogen Receptor α, clone 60C -2572517 2572517
      Anti-Estrogen Receptor α, clone 60C -2649097 2649097
      Anti-Estrogen Receptor α, clone 60C -2667172 2667172
      Anti-Estrogen Receptor α, clone 60C -2724412 2724412


      Reference overviewApplicationPub Med ID
      Resistance to everolimus driven by epigenetic regulation of MYC in ER+ breast cancers.
      Bihani, T; Ezell, SA; Ladd, B; Grosskurth, SE; Mazzola, AM; Pietras, M; Reimer, C; Zinda, M; Fawell, S; D'Cruz, CM
      Oncotarget 6 2407-20 2015

      Show Abstract
      25537515 25537515
      Unraveling the regulatory connections between two controllers of breast cancer cell fate.
      Lee, J; Tiwari, A; Shum, V; Mills, GB; Mancini, MA; Igoshin, OA; Balázsi, G
      Nucleic acids research 42 6839-49 2014

      Show Abstract
      Fluorescence Activated Cell Sorting (FACS)24792166 24792166
      Defining estrogenic mechanisms of bisphenol A analogs through high throughput microscopy-based contextual assays.
      Stossi, F; Bolt, MJ; Ashcroft, FJ; Lamerdin, JE; Melnick, JS; Powell, RT; Dandekar, RD; Mancini, MG; Walker, CL; Westwick, JK; Mancini, MA
      Chemistry & biology 21 743-53 2014

      Show Abstract
      24856822 24856822
      JMJD6 regulates ERα methylation on arginine.
      Poulard, C; Rambaud, J; Hussein, N; Corbo, L; Le Romancer, M
      PloS one 9 e87982 2014

      Show Abstract
      24498420 24498420
      Coactivators enable glucocorticoid receptor recruitment to fine-tune estrogen receptor transcriptional responses.
      Bolt, MJ; Stossi, F; Newberg, JY; Orjalo, A; Johansson, HE; Mancini, MA
      Nucleic acids research 41 4036-48 2013

      Show Abstract
      Immunocytochemistry23444138 23444138
      FDA-approved selective estrogen receptor modulators inhibit Ebola virus infection.
      Johansen, LM; Brannan, JM; Delos, SE; Shoemaker, CJ; Stossel, A; Lear, C; Hoffstrom, BG; Dewald, LE; Schornberg, KL; Scully, C; Lehár, J; Hensley, LE; White, JM; Olinger, GG
      Science translational medicine 5 190ra79 2013

      Show Abstract
      23785035 23785035
      Activation of rapid oestrogen signalling in aggressive human breast cancers.
      Poulard, C; Treilleux, I; Lavergne, E; Bouchekioua-Bouzaghou, K; Goddard-Léon, S; Chabaud, S; Trédan, O; Corbo, L; Le Romancer, M
      EMBO molecular medicine 4 1200-13 2012

      Show Abstract
      23065768 23065768
      High content imaging-based assay to classify estrogen receptor-α ligands based on defined mechanistic outcomes.
      Ashcroft, FJ; Newberg, JY; Jones, ED; Mikic, I; Mancini, MA
      Gene 477 42-52 2011

      Show Abstract
      21256200 21256200
      Activation function 2 (AF2) of estrogen receptor-alpha is required for the atheroprotective action of estradiol but not to accelerate endothelial healing.
      Billon-Galés, A; Krust, A; Fontaine, C; Abot, A; Flouriot, G; Toutain, C; Berges, H; Gadeau, AP; Lenfant, F; Gourdy, P; Chambon, P; Arnal, JF
      Proceedings of the National Academy of Sciences of the United States of America 108 13311-6 2011

      Show Abstract
      21788522 21788522
      ATBF1 inhibits estrogen receptor (ER) function by selectively competing with AIB1 for binding to the ER in ER-positive breast cancer cells.
      Dong, Xue-Yuan, et al.
      J. Biol. Chem., 285: 32801-9 (2010) 2010

      Show Abstract
      20720010 20720010

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      Life Science Research > Antibodies and Assays > Primary Antibodies