A part of MilliporeSigma

05-480 | Anti-Eck/EphA2 Antibody, clone D7

200 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, B, Ca ICC, IP, EA, WB M Purified Monoclonal Antibody
      Catalogue Number05-480
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Eck/EphA2 Antibody, clone D7
      Alternate Names
      • EPH receptor A2
      • Epithelial cell kinase
      • Tyrosine-protein kinase receptor ECK
      • ephrin receptor EphA2
      • epithelial cell receptor protein tyrosine kinase
      • protein tyrosine kinase
      • receptor protein tyrosine kinase regulated by p53 and E2F-1
      Background InformationEPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.
      Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation.
      Product Information
      • A431 cell lysate, CHO cell lysate, foreskin fibroblasts, 3T3/A31 cells, L6 cells.
        Included Positive Antigen Control: Catalog # 12-301, non stimulated A431 lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.
      PresentationPurified mouse monoclonal IgG1 in buffer containing 0.2 M Tris-glycine, pH 7.4, 0.15 M NaCl, with 0.05% sodium azide and 30% glycerol.
      ApplicationThis Anti-Eck/EphA2 Antibody, clone D7 is validated for use in IC, IP, EA, WB for the detection of Eck/EphA2.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Enzyme Assay
      • Western Blotting
      Application NotesProtein Kinase Assay:
      A previous lot of this antibody has been reported to have been used in an immunoprecipitation autophosphorylation assay, using a Mn-PIPES reaction buffer (Romer, L., 1994).

      A previous lot of this antibody has been reported to immunoprecipitate Eck from 500 µg of a human breast epithelial cell line which had been lysed in TBS containing 1% Triton X-100. Use 1-4 µg per reaction.

      A previous lot of this antibody has been reported to immunostain Eck in human, mouse and rat epithelial cells fixed with 3.7% formaldehyde solution and permeabilized with 0.5% Triton X-100 in TBS.
      Biological Information
      ImmunogenNative protein isolated by purification of phosphotyrosine-containing proteins. Clone D7.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes Epithelial Cell Kinase (Eck/EphA2), Mr 140 kDa
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Bovine
      • Canine
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands.
      Gene Symbol
      • EPHA2
      • EphA2
      • ECK
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P29317 # Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A3, -A4 and -A5.
      SIZE: 976 amino acids; 108254 Da
      SUBUNIT: Interacts with SLA (By similarity). Interacts with INPPL1/SHIP2.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g., skin, intestine, lung, and ovary.
      SIMILARITY: SwissProt: P29317 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. & Contains 2 fibronectin type-III domains. & Contains 1 protein kinase domain. & Contains 1 SAM (sterile alpha motif) domain.
      Molecular Weight140 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot on RIPA lysates from human A431, foreskin fibroblasts, murine 3T3/A31 or rat L6 cells.

      Western Blot Analysis:
      0.5-2 µg/mL of this lot detected Eck in RIPA lysates from human A431 and previously from foreskin fibroblasts, murine 3T3/A31 and rat L6 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations:
      Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. NOTE: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Eck/EphA2, clone D7 (mouse monoclonal IgG1) - DAM1400115 DAM1400115
      Anti-Eck/EphA2, clone D7 - 2144399 2144399
      Anti-Eck/EphA2, clone D7 - 2383522 2383522
      Anti-Eck/EphA2, clone D7 - 17038 17038
      Anti-Eck/EphA2, clone D7 - 1993900 1993900
      Anti-Eck/EphA2, clone D7 - 2231276 2231276
      Anti-Eck/EphA2, clone D7 - 23701 23701
      Anti-Eck/EphA2, clone D7 - 31660 31660
      Anti-Eck/EphA2, clone D7 - 32213 32213
      Anti-Eck/EphA2, clone D7 - 33592 33592
      Anti-Eck/EphA2, clone D7 - DAM1400115 DAM1400115
      Anti-Eck/EphA2, clone D7 - DAM1524380 DAM1524380
      Anti-Eck/EphA2, clone D7 - DAM1604953 DAM1604953
      Anti-Eck/EphA2, clone D7 - DAM1718040 DAM1718040
      Anti-Eck/EphA2, clone D7 - DAM1774673 DAM1774673
      Anti-Eck/EphA2, clone D7 - JBC1378218 JBC1378218
      Anti-Eck/EphA2, clone D7 - JBC1900496 JBC1900496
      Anti-Eck/EphA2, clone D7 - JBC1936146 JBC1936146
      Anti-Eck/EphA2, clone D7 -2506412 2506412
      Anti-Eck/EphA2, clone D7 -2583372 2583372
      Anti-Eck/EphA2, clone D7 -2683848 2683848
      Anti-Eck/EphA2, clone D7 -2700793 2700793
      Anti-Eck/EphA2, clone D7 -2739146 2739146
      Anti-Eck/EphA2, clone D7 -2780448 2780448

      References | 20 Available | See All References

      Reference overviewApplicationPub Med ID
      2'-OMe-phosphorodithioate-modified siRNAs show increased loading into the RISC complex and enhanced anti-tumour activity.
      Wu, SY; Yang, X; Gharpure, KM; Hatakeyama, H; Egli, M; McGuire, MH; Nagaraja, AS; Miyake, TM; Rupaimoole, R; Pecot, CV; Taylor, M; Pradeep, S; Sierant, M; Rodriguez-Aguayo, C; Choi, HJ; Previs, RA; Armaiz-Pena, GN; Huang, L; Martinez, C; Hassell, T; Ivan, C; Sehgal, V; Singhania, R; Han, HD; Su, C; Kim, JH; Dalton, HJ; Kovvali, C; Keyomarsi, K; McMillan, NA; Overwijk, WW; Liu, J; Lee, JS; Baggerly, KA; Lopez-Berestein, G; Ram, PT; Nawrot, B; Sood, AK
      Nature communications 5 3459 2014

      Show Abstract
      Western Blotting24619206 24619206
      An integrated genomic approach identifies persistent tumor suppressive effects of transforming growth factor-β in human breast cancer.
      Sato, M; Kadota, M; Tang, B; Yang, HH; Yang, YA; Shan, M; Weng, J; Welsh, MA; Flanders, KC; Nagano, Y; Michalowski, AM; Clifford, RJ; Lee, MP; Wakefield, LM
      Breast cancer research : BCR 16 R57 2014

      Show Abstract
      24890385 24890385
      EphA2-induced angiogenesis in ewing sarcoma cells works through bFGF production and is dependent on caveolin-1.
      Sáinz-Jaspeado, M; Huertas-Martinez, J; Lagares-Tena, L; Martin Liberal, J; Mateo-Lozano, S; de Alava, E; de Torres, C; Mora, J; Del Muro, XG; Tirado, OM
      PloS one 8 e71449 2013

      Show Abstract
      23951165 23951165
      Early insights into the function of KIAA1199, a markedly overexpressed protein in human colorectal tumors.
      Tiwari, A; Schneider, M; Fiorino, A; Haider, R; Okoniewski, MJ; Roschitzki, B; Uzozie, A; Menigatti, M; Jiricny, J; Marra, G
      PloS one 8 e69473 2013

      Show Abstract
      23936024 23936024
      The ephrin receptor tyrosine kinase A2 is a cellular receptor for Kaposi's sarcoma–associated herpesvirus.
      Hahn, AS; Kaufmann, JK; Wies, E; Naschberger, E; Panteleev-Ivlev, J; Schmidt, K; Holzer, A; Schmidt, M; Chen, J; König, S; Ensser, A; Myoung, J; Brockmeyer, NH; Stürzl, M; Fleckenstein, B; Neipel, F
      Nature medicine 18 961-6 2012

      Show Abstract
      22635007 22635007
      Vasculogenic mimicry and its clinical significance in medulloblastoma.
      Shi-Yong Wang,Li Yu,Geng-Qiang Ling,Sha Xiao,Xin-Lin Sun,Zhen-Hua Song,Yi-Jing Liu,Xiao-Dan Jiang,Ying-Qian Cai,Yi-Quan Ke
      Cancer biology & therapy 13 2012

      Show Abstract
      22258034 22258034
      Using patterned supported lipid membranes to investigate the role of receptor organization in intercellular signaling.
      Pradeep M Nair,Khalid Salaita,Rebecca S Petit,Jay T Groves
      Nature protocols 6 2011

      Show Abstract
      21455188 21455188
      EphA2 as a promoter of melanoma tumorigenicity.
      Margaryan, Naira, et al.
      Cancer Biol. Ther., 8: (2009) 2009

      19223760 19223760
      Kinase-dependent and -independent roles of EphA2 in the regulation of prostate cancer invasion and metastasis.
      Taddei, Maria Letizia, et al.
      Am. J. Pathol., 174: 1492-503 (2009) 2009

      Show Abstract
      19264906 19264906
      Regulation of mammary gland branching morphogenesis by EphA2 receptor tyrosine kinase.
      Vaught, David, et al.
      Mol. Biol. Cell, 20: 2572-81 (2009) 2009

      Show Abstract
      19321667 19321667

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