DR1093 Anti-ETV6 Mouse mAb (3B10)

DR1093
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      DR1093-100UG
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          100 μg
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          Description
          OverviewRecognizes the ~55-60 kDa ETV6 protein in Jurkat cells and human placenta tissue.
          Catalogue NumberDR1093
          Brand Family Calbiochem®
          SynonymsAnti-ETS Variant 6
          Application Data
          Detection of human EVT6 by immunoblotting. Sample: Whole cell lysate from Jurkat cells. Primary antibody: Anti-ETV6 Mouse mAb (3B10) (Cat. No. DR1093) (1-5 µg/ml). Detection: chemilimunescence.

          Detection of human EVT6 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human placenta tissue. Primary antibody: Anti-ETV6 Mouse mAb (3B10) (Cat. No. DR1093) (3 µg/ml). Detection: colorimetric (DAB).
          References
          Product Information
          FormLiquid
          FormulationIn PBS, pH 7.2.
          Positive controlJurkat cells, Human placenta tissue
          PreservativeNone
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Paraffin Sections
          Application NotesELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Paraffin Sections (3 µg/ml)
          Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          ImmunogenFull-length, recombinant, human ETV6 (aa 1-453), expressed as a GST fusion protein
          Clone3B10
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Regulatory Review
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-ETV6 Mouse mAb (3B10) SDS

          Title

          Safety Data Sheet (SDS) 

          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision04-November-2010 RFH
          SynonymsAnti-ETS Variant 6
          ApplicationELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Paraffin Sections (3 µg/ml)
          Application Data
          Detection of human EVT6 by immunoblotting. Sample: Whole cell lysate from Jurkat cells. Primary antibody: Anti-ETV6 Mouse mAb (3B10) (Cat. No. DR1093) (1-5 µg/ml). Detection: chemilimunescence.

          Detection of human EVT6 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human placenta tissue. Primary antibody: Anti-ETV6 Mouse mAb (3B10) (Cat. No. DR1093) (3 µg/ml). Detection: colorimetric (DAB).
          DescriptionPurified mouse monoclonal antibody. Recognizes the ~55-60 kDa ETV6 protein.
          BackgroundETV6 contains two functional domains: a N-terminal pointed (PNT) domain that is involved in protein-protein interactions with itself and other proteins, and a C-terminal DNA-binding domain. Gene knockout studies in mice suggest that this protein is required for hematopoiesis and maintenance of the developing vascular network. This gene is known to be involved in a large number of chromosomal rearrangements associated with leukemia and congenital fibrosarcoma.
          HostMouse
          ImmunogenFull-length, recombinant, human ETV6 (aa 1-453), expressed as a GST fusion protein
          Clone3B10
          IsotypeIgG₁
          Specieshuman
          Positive controlJurkat cells, Human placenta tissue
          FormLiquid
          FormulationIn PBS, pH 7.2.
          PreservativeNone
          CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Regulatory Review