ABS1611 | Anti-ERLIN1/SPFH1 Antibody, C-terminus

ABS1611
200 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R WB, ICC, IP Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberABS1611
      DescriptionAnti-ERLIN1/SPFH1 Antibody, C-terminus
      Alternate Names
      • ER lipid raft associated 1
      • Protein Erlin1
      • SPFH domain family, member 1
      Background InformationER lipid raft associated 1 (UniProt B1WBY7; also known as Protein Erlin1, SPFH domain family, member 1) is encoded by the Erlin1 (also known as RGD1307058 or Spfh1_predicted) gene in rat (Entrez Gene ID 293939; ORF rCG_57742). Erlin1 (Spfh1) and the highly homologous Erlin2 (Spfh2) are ER proteins that hetero-oligomerize into a large complex of ∼2 MDa. The erlin1/2 complex associates with inositol 1,4,5-trisphosphate (IP(3)) receptors on ER membrane and is responsible for recruiting RNF170 upon IP(3) receptor activation for subsequent ubiquitination-dependent receptor downregulation (PMID 19240031, 21610068, 22020079). Cat. No. ABS1611, ERLIN1/SPFH1, C-terminus, is an affinity purified rabbit polyclonal antibody against rat Erlin1/Spfh1 C-terminal end sequence. This polyclonal antibody is reactive toward rat and murine, but not human, species (PMID 19240031).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 155 mM NaCl, 50 mM Tris-HCl pH 7.4, with 0.05% sodium azide and 10% glycerol.
      Applications
      ApplicationAnti-ERLIN1/SPFH1 Antibody, C-terminus is an antibody against ERLIN1/SPFH1, C-terminus for use in Western Blotting, Immunocytochemistry, Immunoprecipitation.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesWestern Blotting Analysis: A representative lot detected 100 nM GnRH-induced ERLIN1/SPFH1 association with IP3R1 in alphaT3-1 cells, as well as 10 nM ET1-induced ERLIN1/SPFH1 association with IP3R1 in Rat-1 cells (Pearce, M.M., et al. (2009) J. Biol. Chem. 284:10433-10445).
      Immunocytochemistry Analysis: A representative lot detected ERLIN1/SPFH1 in Rat1 cells (Pearce, M.M., et al. (2009) J. Biol. Chem. 284:10433-10445).
      Immunoprecipitation Analysis: A representative lot immunoprecipitated ERLIN1/SPFH1 in alphaT3-1 cells (Pearce, M.M., et al. (2009) J. Biol. Chem. 284:10433-10445).
      Biological Information
      ImmunogenLinear peptide corresponding to the C-terminus of rat ERLIN1/SPFH1.
      EpitopeC-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      Species Reactivity
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Erlin1
      • Spfh1_predicted
      • rCG_57742
      Purification MethodAffinity
      UniProt Number
      Molecular Weight~43 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MEF-1 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected ERLIN1/SPFH1 in 10 µg of MEF-1 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-ERLIN1/SPFH1, C-terminus -Q2564187 Q2564187

      References

      Reference overviewPub Med ID
      An endoplasmic reticulum (ER) membrane complex composed of SPFH1 and SPFH2 mediates the ER-associated degradation of inositol 1,4,5-trisphosphate receptors.
      Pearce, MM; Wormer, DB; Wilkens, S; Wojcikiewicz, RJ
      The Journal of biological chemistry  284  10433-45  2009

      Show Abstract
      19240031 19240031

      Brochure

      Title
      NPI Flyer- Epigenetics and Nuclear Function Feature (EMD)
      New Products Flyer, Volume 2, 2015, Immunology Feature (EMD)

      Technical Info

      Title
      White Paper: Further considerations of antibody validation and usage. (EMD)