A part of MilliporeSigma

MAB447 | Anti-ER-alpha Antibody, clone AER314

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H, M, R, Rb IP, WB, IH(P) M Ascites Monoclonal Antibody
      Catalogue NumberMAB447
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-ER-alpha Antibody, clone AER314
      Alternate Names
      • ER
      • ER1
      • Estradiol Receptor
      • ER-alpha
      Product Information
      ApplicationUse Anti-ER-alpha Antibody, clone AER314 (Mouse Monoclonal Antibody) validated in IP, WB, IHC(P) to detect Estrogen Receptor α also known as ER, ER1, Estradiol Receptor, ER-alpha.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern blot (human)

      Immunoprecipitation (human)

      Immunohistochemistry (mouse): faint staining

      Immunohistochemistry (human): reactive on paraffin embedded sections after microwaving. *Some cytoplasmic staining may be seen.

      Final working dilutions must be determined by end user.
      Biological Information
      ImmunogenBovine estrogen receptor.
      Epitopea.a. 120-170 of hER-1 alpha
      SpecificityEstrogen receptor 67kD hormone binding subunit. Epitope localized between aa 120-170.


      0.8 nM
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Rat
      • Rabbit
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters.
      Gene Symbol
      • ESR1
      • ER-alpha
      • ESR
      • Era
      • ER
      • NR3A1
      • ESRA
      • DKFZp686N23123
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P03372 # Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.
      SIZE: 595 amino acids; 66216 Da
      SUBUNIT: Binds DNA as a homodimer. Can form a heterodimer with ESR2. Interacts with NCOA3, NCOA5 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with NCOA7 in a ligand-inducible manner. Interacts with PHB2, PELP1 and UBE1C. Interacts with AKAP13. Interacts with CUEDC2. Interacts with JARID1A. Interacts with SMARD1. Interacts with SLC30A9 (By similarity). Interacts with HEXIM1 and MAP1S. Interacts with PBXIP1.
      DOMAIN: SwissProt: P03372 Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain.
      PTM: Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
      SIMILARITY: Belongs to the nuclear hormone receptor family. NR3 subfamily. & Contains 1 nuclear receptor DNA-binding domain.
      MISCELLANEOUS: In the absence of ligand, steroid hormone receptors are thought to be weakly associated with nuclear components; hormone binding greatly increases receptor affinity. The hormone- receptor complex appears to recognize discrete DNA sequences upstream of transcriptional start sites.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to one year. Avoid repeated freeze-thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-ESTROGEN RECEPTOR (aa 120-170) -2556289 2556289
      MOUSE ANTI-ESTROGEN RECEPTOR (aa 120-170) -2606256 2606256
      MOUSE ANTI-ESTROGEN RECEPTOR (aa 120-170) -2634181 2634181
      MOUSE ANTI-ESTROGEN RECEPTOR (aa 120-170) -2640530 2640530
      MOUSE ANTI-ESTROGEN RECEPTOR (aa 120-170) -2679419 2679419
      MOUSE ANTI-ESTROGEN RECEPTOR (aa 120-170) -2816678 2816678


      Reference overviewPub Med ID
      Histopathological, ultrastructural, and immunohistochemical assessment of hippocampus structures of rats exposed to TCDD and high doses of tocopherol and acetylsalicylic acid.
      Rosińczuk, J; Dymarek, R; Całkosiński, I
      BioMed research international 2015 645603 2015

      Show Abstract
      25879034 25879034
      Young coconut juice can accelerate the healing process of cutaneous wounds.
      Radenahmad, N; Saleh, F; Sayoh, I; Sawangjaroen, K; Subhadhirasakul, P; Boonyoung, P; Rundorn, W; Mitranun, W
      BMC complementary and alternative medicine 12 252 2012

      Show Abstract
      23234369 23234369
      HIV-1 replication in Langerhans and interstitial dendritic cells is inhibited by neutralizing and Fc-mediated inhibitory antibodies.
      M Peressin,V Holl,S Schmidt,T Decoville,D Mirisky,A Lederle,M Delaporte,K Xu,A M Aubertin,C Moog
      Journal of virology 85 2011

      Show Abstract Full Text Article
      21084491 21084491
      Intraprotomer masking of third variable loop (V3) epitopes by the first and second variable loops (V1V2) within the native HIV-1 envelope glycoprotein trimer.
      Li Liu,Raffaello Cimbro,Paolo Lusso,Edward A Berger
      Proceedings of the National Academy of Sciences of the United States of America 108 2011

      Show Abstract Full Text Article
      22128330 22128330
      Estrogen-induced dimerization and activation of ERα-fused caspase-8: artificial reversal of the estrogenic hormone effect in carcinogenesis.
      Zhi-Ning Zhao,Qiang Zhou,Jiu-Xu Bai,Bo Yan,Wei-Wei Qin,Tao Wang,Yi Li,An-Gang Yang,Lin-Tao Jia
      Cancer biology & therapy 11 2011

      Show Abstract
      21364316 21364316
      Influence of novel CD4 binding-defective HIV-1 envelope glycoprotein immunogens on neutralizing antibody and T-cell responses in nonhuman primates.
      Iyadh Douagi,Mattias N E Forsell,Christopher Sundling,Sijy O'Dell,Yu Feng,Pia Dosenovic,Yuxing Li,Robert Seder,Karin Loré,John R Mascola,Richard T Wyatt,Gunilla B Karlsson Hedestam
      Journal of virology 84 2010

      Show Abstract Full Text Article
      19955308 19955308
      Estrogen regulation of vascular endothelial growth factor in breast cancer in vitro and in vivo: the role of estrogen receptor alpha and c-Myc.
      M Dadiani, D Seger, T Kreizman, D Badikhi, R Margalit, R Eilam, H Degani
      Endocrine-related cancer 16 819-34 2009

      Show Abstract
      19398483 19398483
      Estradiol-estrogen receptor: a key interplay of the expression of syndecan-2 and metalloproteinase-9 in breast cancer cells.
      Olga Ch Kousidou,Aikaterini Berdiaki,Dimitris Kletsas,Alexandros Zafiropoulos,Achilleas D Theocharis,George N Tzanakakis,Nikos K Karamanos
      Molecular oncology 2 2008

      Show Abstract
      19383343 19383343
      Infectivity and neutralization of simian immunodeficiency virus with FLAG epitope insertion in gp120 variable loops.
      Melissa E Laird,Ronald C Desrosiers
      Journal of virology 81 2007

      Show Abstract Full Text Article
      17686865 17686865

      Data Sheet


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