AP1173 Anti-EPHA4 Mouse mAb (6H7)

AP1173
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      AP1173-100UG
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          100 μg
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          Description
          OverviewRecognizes the EPHA4 protein in NIH3T3 cells.
          Catalogue NumberAP1173
          Brand Family Calbiochem®
          SynonymsAnti-Ephrin Receptor A4
          Application Data
          Detection of mouse EPHA4 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-EPHA4 Mouse mAb (6H7) (Cat. No. AP1173) (1-5 µg/ml). Detection: chemiluminescence.
          References
          Product Information
          FormLiquid
          FormulationIn PBS, pH 7.2.
          Positive controlNIH3T3 cells
          PreservativeNone
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Application NotesELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          ImmunogenA recombinant polypeptide corresponding to amino acids of 887-987 human EPHA4, expressed as a GST fusion protein
          Clone6H7
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          • Mouse
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Regulatory Review
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-EPHA4 Mouse mAb (6H7) SDS

          Title

          Safety Data Sheet (SDS) 

          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision19-October-2010 RFH
          SynonymsAnti-Ephrin Receptor A4
          ApplicationELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Application Data
          Detection of mouse EPHA4 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-EPHA4 Mouse mAb (6H7) (Cat. No. AP1173) (1-5 µg/ml). Detection: chemiluminescence.
          DescriptionPurified mouse monoclonal antibody. Recognizes the ~100 kDa EPHA4 protein.
          BackgroundThis protein belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.
          HostMouse
          ImmunogenA recombinant polypeptide corresponding to amino acids of 887-987 human EPHA4, expressed as a GST fusion protein
          Clone6H7
          IsotypeIgG₁
          Specieshuman, mouse
          Positive controlNIH3T3 cells
          FormLiquid
          FormulationIn PBS, pH 7.2.
          PreservativeNone
          CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Regulatory Review